A CO2 incubator yana samar da iska, shin dangi zafi yayi yawa?
Lokacin da muka yi amfani da CO2 incubator don noma sel, saboda bambancin yawan adadin ruwa da aka kara da kuma al'ada, muna da buƙatu daban-daban don ƙarancin dangi a cikin incubator.
Don gwaje-gwajen da aka yi amfani da faranti na al'adun cell 96 mai tsayi mai tsayi mai tsayi, saboda ƙananan adadin ruwa da aka saka a cikin rijiyar guda ɗaya, akwai haɗarin cewa maganin al'ada zai bushe idan ya kwashe tsawon lokaci a 37 ℃.
Higher dangi zafi a cikin incubator, misali, don isa fiye da 90%, iya yadda ya kamata rage evaporation na ruwa, duk da haka, wata sabuwar matsala ta taso, da yawa cell al'adun gwaji gwaje-gwaje sun gano cewa incubator ne mai sauki don samar da condensate a high zafi yanayi, condensate samar idan ba a sarrafa ba, zai tara da yawa, zuwa ga cell al'ada ya kawo wani hadarin kamuwa da cuta.
Don haka, shin ƙarni na narkar da iska a cikin incubator ne saboda ƙarancin dangi ya yi yawa?
Da farko, muna bukatar mu fahimci manufar dangi zafi,zafi dangi (Labarin Humidity, RH)shine ainihin abin da ke cikin tururin ruwa a cikin iska da kuma adadin yawan tururin ruwa a jikewa a yanayin zafi ɗaya. An bayyana a cikin dabara:
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Yawan zafi na dangi yana wakiltar rabon abun ciki na tururin ruwa a cikin iska zuwa matsakaicin abun ciki mai yuwuwa.
Musamman:
0% RH:Babu tururin ruwa a cikin iska.
100% RH:Iskar ta cika da tururin ruwa kuma ba za ta iya ɗaukar ƙarin tururin ruwa ba kuma za a yi tari.
50% RH:Yana nuna cewa yawan tururin ruwa a halin yanzu a cikin iska shine rabin adadin tururin ruwa mai cike da zafin jiki. Idan zafin jiki ya kasance 37 ° C, to, madaidaicin tururin ruwa yana kusan 6.27 kPa. Saboda haka, matsa lamba na ruwa a 50% zafi dangi shine kusan 3.135 kPa.
Cikakkun tururin ruwashine matsi da tururi ke haifarwa a lokacin iskar gas lokacin da ruwa mai ruwa da tururinsa ke cikin ma'auni mai ƙarfi a wani yanayin zafi.
Musamman, lokacin da tururin ruwa da ruwan ruwa suka kasance tare a cikin rufaffiyar tsarin (misali, Radobio CO2 incubator da aka rufe da kyau), kwayoyin ruwa za su ci gaba da canzawa daga yanayin ruwa zuwa yanayin gaseous (evaporation) na tsawon lokaci, yayin da kuma kwayoyin ruwa na gas za su ci gaba da canzawa zuwa yanayin ruwa (condensation).
A wani lokaci, ƙimar ƙawancen ƙawance da ƙugiya daidai suke, kuma tururin tururi a wannan lokacin shine matsewar tururin ruwa. An siffanta shi da
1. ma'auni mai ƙarfi:a lokacin da ruwa da tururin ruwa ke zama tare a cikin rufaffiyar tsarin, tururi da tari don isa ga daidaito, matsa lamba na tururin ruwa a cikin tsarin ba ya canzawa, a wannan lokacin matsa lamba yana cike da matsa lamba na ruwa.
2. dogaro da zafin jiki:cikakken tururin ruwa yana canzawa tare da zafin jiki. Lokacin da zafin jiki ya ƙaru, ƙarfin motsi na ƙwayoyin ruwa yana ƙaruwa, ƙarin ƙwayoyin ruwa na iya tserewa zuwa lokacin iskar gas, don haka matsin tururin ruwa yana ƙaruwa. Akasin haka, lokacin da zafin jiki ya ragu, madaidaicin tururin ruwa yana raguwa.
3. Halaye:Cikakkun matsa lamba na ruwa shine sigar siffa ta kayan abu kawai, baya dogara da adadin ruwa, kawai tare da zafin jiki.
Wata dabara ta gama gari da ake amfani da ita don ƙididdige matsin tururin ruwa mai cike da ruwa shine ma'aunin Antoine:
Don ruwa, kullun Antoine yana da ƙima daban-daban don kewayon zafin jiki daban-daban. Saitin na yau da kullun shine:
A=8.07131
B=1730.63
C=233.426
Wannan saitin madaidaicin ya shafi kewayon zafin jiki daga 1 ° C zuwa 100 ° C.
Za mu iya amfani da waɗannan madaidaicin don ƙididdige cewa cikakken ruwa a 37 ° C shine 6.27 kPa.
Don haka, nawa ne ruwa a cikin iska a digiri 37 a ma'aunin celcius (°C) a cikin yanayin matsananciyar tururin ruwa?
Don ƙididdige yawan abun ciki na cikakken tururin ruwa (cikakkiyar zafi), zamu iya amfani da dabarar lissafin Clausius-Clapeyron:
Cikakkar matsa lamba na tururin ruwa: A 37 ° C, cikakken tururin ruwa shine 6.27 kPa.
Canza yanayin zafi zuwa Kelvin: T=37+273.15=310.15 K
Sauya cikin dabara:
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Sakamakon da aka samu ta hanyar lissafi shine kusan 44.6 g/m³.
A 37 ° C, abin da ke cikin tururin ruwa (cikakkiyar zafi) a jikewa ya kai 44.6 g/m³. Wannan yana nufin cewa kowace mita cubic na iska na iya ɗaukar gram 44.6 na tururin ruwa.
A 180L CO2 incubator zai riƙe kusan gram 8 na tururin ruwa kawai.Lokacin da kwanon rufi da tasoshin al'adu suka cika da ruwaye, yanayin zafi na dangi zai iya kaiwa ga maɗaukakin ƙima cikin sauƙi, har ma kusa da ƙimar zafi.
Lokacin da dangi zafi ya kai 100%,tururin ruwa ya fara takurawa. A wannan lokacin, adadin tururin ruwa a cikin iska ya kai iyakar ƙimar da zai iya ɗauka a yanayin zafin da ake ciki yanzu, watau jikewa. Ƙarin haɓakar tururin ruwa ko raguwar zafin jiki yana haifar da tururin ruwa zuwa cikin ruwa mai ruwa.
Hakanan na iya faruwa lokacin da yanayin zafi ya wuce 95%,amma wannan ya dogara da wasu dalilai kamar zafin jiki, yawan tururin ruwa a cikin iska, da kuma yanayin zafi. Wadannan abubuwa masu tasiri sune kamar haka:
1. Rage zafin jiki:Lokacin da yawan tururin ruwa a cikin iska yana kusa da jikewa, duk wani ƙaramin raguwar zafin jiki ko ƙara yawan tururin ruwa na iya haifar da kumburi. Alal misali, yawan zafin jiki a cikin incubator na iya haifar da samar da condensate, don haka yawan zafin jiki ya fi dacewa da incubator zai sami tasiri mai hanawa a kan samar da condensate.
2. zafin jiki na gida ƙasa da zafin raɓa:Yanayin zafin jiki na gida ya fi ƙasa da zafin raɓa, tururin ruwa zai taso cikin ɗigon ruwa a kan waɗannan saman, don haka daidaiton zafin jiki na incubator zai sami kyakkyawan aiki a cikin hana ƙura.
3. Yawan tururin ruwa:alal misali, kwanon humidification da kwantena na al'ada tare da ruwa mai yawa, kuma injin incubator yana da kyau a rufe, lokacin da yawan tururin ruwa a cikin iska a cikin incubator ya karu fiye da iyakar ƙarfinsa a yanayin zafi na yanzu, ko da yanayin zafi ya kasance bai canza ba, za a haifar da tari.
Sabili da haka, CO2 incubator tare da kula da zafin jiki mai kyau a fili yana da tasiri mai hanawa a kan samar da condensate, amma lokacin da yanayin zafi ya wuce 95% ko ma ya kai ga jikewa, yuwuwar kamuwa da cuta zai karu sosai.sabili da haka, lokacin da muke noma sel, ban da zabar incubator mai kyau na CO2, ya kamata mu yi ƙoƙari mu guje wa hadarin daɗaɗɗen da ake samu ta hanyar bin babban zafi.
Lokacin aikawa: Yuli-23-2024