Yadda za a Zaɓi Madaidaicin Shaker Amplitude?
Menene girman girman mai girgiza?
Girman girman mai girgiza shine diamita na pallet a cikin motsi na madauwari, wani lokaci ana kiranta "diamita na oscillation" ko "diamita waƙa" alama: Ø. Radobio yana ba da madaidaicin shaker tare da amplitudes na 3mm, 25mm, 26mm da 50mm,. Hakanan ana samun maɓalli na musamman tare da sauran girman girman girma.
Menene Canja wurin Oxygen (OTR)?
Oxygen Transfer Rate (OTR) shine ingancin iskar oxygen da ake canjawa wuri daga yanayi zuwa ruwa. Mafi girman ƙimar OTR yana nufin mafi girman ƙimar iskar oxygen.
Tasirin Girma da Gudun Juyawa
Duk waɗannan abubuwan biyu suna shafar haɗuwa da matsakaici a cikin flask ɗin al'ada. Mafi kyawun hadawa, mafi kyawun canjin iskar oxygen (OTR). Bi waɗannan jagororin, za'a iya zaɓar mafi dacewa girman girma da juyawa.
Gabaɗaya, zaɓin 25mm ko 26mm amplitude ana iya amfani dashi azaman girman girman duniya don duk aikace-aikacen al'adu.
Bacterial, yisti da fungal al'adun:
Canja wurin iskar oxygen a cikin filayen shake ba shi da inganci sosai fiye da na masu sarrafa halittu. Canja wurin iskar oxygen na iya zama abin iyakance ga al'adun flask a mafi yawan lokuta. Girman yana da alaƙa da girman ƙwanƙolin ƙwanƙwasa: manyan flasks suna amfani da amplitudes masu girma.
Shawarwari: 25mm amplitude don conical flasks daga 25ml zuwa 2000ml.
50 mm amplitude don conical flasks daga 2000 ml zuwa 5000 ml.
Al'adun Kwayoyin Halitta:
* Al'adun sel masu shayarwa suna da ƙarancin buƙatun iskar oxygen.
* Domin 250mL shaker flasks, isasshen iskar oxygen za a iya bayar a kan in mun gwada da fadi da kewayon amplitudes da gudu (20-50mm amplitude; 100-300rpm).
* Don manyan filayen diamita (Fernbach flasks) ana ba da shawarar girman 50mm.
* Idan ana amfani da jakunkuna na al'ada, ana ba da shawarar girman 50mm.
Microtiter da faranti mai zurfi:
Don microtiter da faranti mai zurfi akwai hanyoyi daban-daban guda biyu don samun matsakaicin iskar oxygen!
* 50 mm amplitude a gudun ba kasa da 250 rpm.
* Yi amfani da amplitude 3mm a 800-1000rpm.
A lokuta da yawa, ko da an zaɓi amplitude mai ma'ana, maiyuwa ba zai ƙara ƙarar ƙwayar halitta ba, saboda haɓakar ƙarar na iya yin tasiri da abubuwa da yawa. Alal misali, idan ɗaya ko biyu daga cikin abubuwan goma ba su dace ba, to, haɓakar ƙarar al'ada za ta iyakance ko ta yaya sauran abubuwan suke da kyau, ko kuma ana iya jayayya cewa daidaitaccen zaɓi na amplitude zai haifar da karuwa mai girma a cikin incubator idan kawai abin da ke iyakance ga girman al'ada shine isar da iskar oxygen. Alal misali, idan tushen carbon shine ƙayyadaddun abu, komai kyawun iskar iskar oxygen, ba za a samu girman al'adun da ake so ba.
Girma da Gudun Juyawa
Duk girman girman da saurin juyawa na iya yin tasiri akan canja wurin iskar oxygen. Idan al'adun tantanin halitta suna girma a cikin ƙananan saurin juyawa (misali, 100 rpm), bambance-bambance a cikin amplitude ba su da ɗan tasiri ko tasiri akan canja wurin oxygen. Don cimma mafi girman canja wurin iskar oxygen, mataki na farko shine ƙara saurin juzu'i kamar yadda zai yiwu, kuma tire zai daidaita daidai don saurin gudu. Ba duk sel ba ne zasu iya girma da kyau tare da babban motsin motsi, kuma wasu sel waɗanda ke da hankali ga ƙarfin juzu'i na iya mutuwa daga saurin jujjuyawa.
Sauran tasiri
Wasu dalilai na iya yin tasiri akan canja wurin oxygen:.
* Cika ƙarar, filayen conical yakamata a cika su zuwa fiye da kashi ɗaya bisa uku na jimlar ƙarar. Idan ana so a cimma iyakar iskar oxygen, cika ba fiye da 10%. Kar a taba cika zuwa 50%.
* Masu ɓarna: Masu ɓarna suna da tasiri wajen haɓaka iskar oxygen a cikin kowane nau'in al'adu. Wasu masana'antun suna ba da shawarar amfani da flasks "Ultra High Yield". Masu ɓarna a kan waɗannan filaye suna ƙara jujjuyawar ruwa kuma mai girgiza ba zai iya kaiwa matsakaicin saurin saitawa ba.
Daidaita tsakanin girman da sauri
Ana iya ƙididdige ƙarfin centrifugal a cikin mai girgiza ta amfani da ma'auni mai zuwa
FC = rpm2× amplitude
Akwai dangantaka ta layi tsakanin ƙarfin centrifugal da amplitude: idan kun yi amfani da girman 25 mm amplitude zuwa girman 50 mm (a irin wannan gudun), ƙarfin centrifugal yana ƙaruwa da kashi 2.
Alakar murabba'i tana wanzu tsakanin ƙarfin tsakiya da saurin juyawa.
Idan an karu da sauri da juzu'i na 2 (daidaitacce), ƙarfin centrifugal yana ƙaruwa da ma'auni na 4. Idan an ƙara saurin da kashi 3, ƙarfin centrifugal yana ƙaruwa da kashi 9!
Idan kun yi amfani da girman 25 mm, kunsa a saurin da aka ba. Idan kuna son cimma ƙarfin centrifugal iri ɗaya tare da girman 50 mm, saurin juyawa yakamata a lissafta shi azaman tushen murabba'in 1/2, don haka yakamata kuyi amfani da 70% na saurin juyawa don cimma yanayin shiryawa iri ɗaya.
Lura cewa abin da ke sama hanya ce ta ka'idar ƙididdige ƙarfin centrifugal kawai. Akwai wasu abubuwa masu tasiri a cikin aikace-aikacen gaske. Wannan hanyar lissafin tana ba da ƙima mai ƙima don dalilai na aiki.
Lokacin aikawa: Satumba-10-2023