Me yasa ake buƙatar CO2 a al'adar tantanin halitta?

Matsakaicin pH na maganin al'adun sel na al'ada yana tsakanin 7.0 da 7.4. Tun da tsarin buffer na carbonate pH shine tsarin tsarin pH na jiki (yana da mahimmancin tsarin buffer pH a cikin jinin mutum), ana amfani dashi don kula da pH mai tsayi a yawancin al'adu. wani adadin sodium bicarbonate sau da yawa yana buƙatar ƙarawa lokacin shirya al'adu tare da foda. Ga mafi yawan al'adun da ke amfani da carbonate a matsayin tsarin buffer pH, don kiyaye tsayayyen pH, carbon dioxide a cikin incubator yana buƙatar kiyayewa tsakanin 2-10% don kula da ƙaddamar da narkar da carbon dioxide a cikin maganin al'ada. A lokaci guda tasoshin al'adun tantanin halitta suna buƙatar zama ɗan numfashi don ba da damar musayar iskar gas.

Shin yin amfani da wasu tsarin buffer pH yana kawar da buƙatar CO2 incubator? An gano cewa saboda ƙarancin ƙwayar carbon dioxide a cikin iska, idan ba a haɓaka ƙwayoyin sel a cikin incubator carbon dioxide ba, HCO3- a cikin matsakaicin al'ada zai ƙare, kuma wannan zai tsoma baki tare da ci gaban al'ada na sel. Don haka yawancin ƙwayoyin dabbobi har yanzu ana al'adarsu a cikin incubator CO2.

A cikin 'yan shekarun da suka gabata, fannonin ilmin halitta, ilmin kwayoyin halitta, ilmin hada magunguna, da dai sauransu sun sami ci gaba mai ban mamaki a cikin bincike, kuma a lokaci guda, amfani da fasaha a cikin wadannan fagage ya zama dole. Kodayake kayan aikin dakin gwaje-gwaje na kimiyyar rayuwa na yau da kullun sun canza sosai, CO2 incubator har yanzu wani muhimmin sashi ne na dakin gwaje-gwaje, kuma ana amfani da shi don manufar kiyayewa da haɓaka haɓakar ƙwayoyin cuta da kyallen takarda. Duk da haka, tare da ci gaba a fasaha, aikin su da aiki sun zama mafi daidai, abin dogara da dacewa. A zamanin yau, CO2 incubators sun zama ɗaya daga cikin kayan aikin yau da kullun da aka saba amfani da su a cikin dakunan gwaje-gwaje kuma an yi amfani da su sosai a cikin bincike da samarwa a cikin magunguna, rigakafi, kwayoyin halitta, microbiology, kimiyyar noma, da harhada magunguna.

A CO2 incubator yana haifar da yanayi don ingantacciyar haɓakar tantanin halitta/nama ta hanyar sarrafa yanayin muhallin da ke kewaye. Sakamakon yanayin kula da yanayin yana haifar da yanayin kwanciyar hankali: misali acidity / alkalinity akai-akai (pH: 7.2-7.4), zafin jiki mai ƙarfi (37 ° C), zafi mai girma (95%), da kuma matakin CO2 mai tsayi (5%), wanda shine dalilin da ya sa masu bincike a cikin filayen da ke sama suna da sha'awar yin amfani da CO2 incubator.

Bugu da ƙari, tare da ƙari na CO2 maida hankali da kuma yin amfani da microcontroller don daidaitaccen kula da zafin jiki na incubator, nasarar nasarar da ingancin noman kwayoyin halitta da kyallen takarda, da dai sauransu, an inganta. A takaice dai, CO2 incubator wani sabon nau'in incubator ne wanda ba za a iya maye gurbinsa da na'urar lantarki ta yau da kullun ba a dakunan gwaje-gwajen halittu.