Kutheni i-CO2 ifuneka kwinkcubeko yeeseli?

I-pH yesisombululo senkcubeko yeseli eqhelekileyo iphakathi kwe-7.0 kunye ne-7.4. Ekubeni inkqubo ye-carbonate pH buffer yinkqubo ye-pH ye-physiological buffer (yinkqubo ebalulekileyo ye-pH ye-buffer egazini lomntu), isetyenziselwa ukugcina i-pH ezinzileyo kwiinkcubeko ezininzi. umlinganiselo othile we-sodium bicarbonate kaninzi kufuneka wongezwe xa ulungiselela iinkcubeko kunye nomgubo. Kwiinkcubeko ezininzi ezisebenzisa i-carbonate njengenkqubo ye-pH ye-buffer, ukuze kugcinwe i-pH ezinzileyo, i-carbon dioxide kwi-incubator kufuneka igcinwe phakathi kwe-2-10% yokugcina i-concentration ye-carbon dioxide edibeneyo kwisisombululo senkcubeko. Kwangaxeshanye iinqanawa zokukhuliswa kweeseli kufuneka ziphefumle kancinci ukuvumela ukutshintshiselana ngerhasi.

Ngaba ukusetyenziswa kwezinye iinkqubo zesithinteli se-pH kuphelisa isidingo se-CO2 incubator? Kuye kwafunyaniswa ukuba ngenxa yoxinzelelo oluphantsi lwekhabhoni diokside emoyeni, ukuba iiseli azikhuliswanga kwi-incubator ye-carbon dioxide, i-HCO3-kwindawo yenkcubeko iya kuphelelwa, kwaye oku kuya kuphazamisa ukukhula okuqhelekileyo kweeseli. Ke uninzi lweeseli zezilwanyana zisakhuliswa kwi-CO2 incubator.

Kule minyaka imbalwa idlulileyo, imimandla yebhayoloji yeeseli, i-molecular biology, i-pharmacology, njl.njl. yenze imitsi emangalisayo kuphando, kwaye kwangaxeshanye, ukusetyenziswa kobuchwephesha kwezi nkalo kuye kwafuneka kuhambisane nesantya. Nangona izixhobo eziqhelekileyo zelebhu yenzululwazi yobomi zitshintshe kakhulu, i-CO2 incubator iseyinxalenye ebalulekileyo yelebhu, kwaye isetyenziselwa injongo yokugcina nokukhuthaza ukukhula okungcono kweeseli kunye nezicubu. Nangona kunjalo, ngokuhambela phambili kwetekhnoloji, umsebenzi wabo kunye nokusebenza kwabo kuye kwachaneka ngakumbi, kunokwethenjelwa kwaye kulula. Namhlanje, i-CO2 incubators ibe sesinye sezixhobo eziqhelekileyo ezisetyenziswa kwiilabhoratri kwaye zisetyenziswe ngokubanzi kuphando kunye nokuveliswa kweyeza, i-immunology, i-genetics, i-microbiology, isayensi yezolimo, kunye ne-pharmacology.

I-incubator ye-CO2 idala indawo engcono yokukhula kweseli / izicubu ngokulawula iimeko ezingqongileyo ezingqongileyo. Isiphumo solawulo lwemeko sidala imeko ezinzileyo: umz. i-acidity eqhubekayo/i-alkalinity (pH: 7.2-7.4), iqondo lobushushu elizinzile (37°C), ukufuma okuphezulu (95%), kunye nezinga le-CO2 elizinzile (5%), yingakho abaphandi kule mimandla ingasentla benomdla wokusebenzisa i-CO2 incubator.

Ukongezelela, kunye nokongezwa kwe-CO2 yolawulo lokuxininisa kunye nokusetyenziswa kwe-microcontroller yokulawula ukushisa okuchanekileyo kwe-incubator, izinga lokuphumelela kunye nokusebenza kakuhle kokulinywa kweeseli ze-biological kunye nezicubu, njl., ziye zaphuculwa. Ngamafutshane, i-CO2 incubator luhlobo olutsha lwe-incubator engenakutshintshwa yi-incubator eqhelekileyo yombane ye-thermostat kwiilebhu zebhayoloji.