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Ingakanani i-medium ekufuneka yongezwe kwiflaski kwi-incubator eshukumayo?
Iiflaski ze-Erlenmeyer zeRADOBIO ezingengomthi zenkcubeko yeeseli ezisetyenzisiweyo zenzelwe ngokukodwa ukukhulisa iiseli ezixhonyiweyo kuquka iiseli ze-HEK 293 kunye ne-CHO kwi-incubator eshukumayo. Ezi flaski zinezantsi elithe tyaba kunye nesigqubuthelo esinomoya, nto leyo eyenza ukuba zilungele uphuhliso lwenkqubo encinci, ukukhulisa, kunye ...Funda ngokugqithisileyo -
Yintoni i-CO₂ Incubator? Isikhokelo esikhawulezayo
Nangona isetyenziswa kakhulu kwisayensi yobomi, imisebenzi ye-CO₂ incubator ayikaqhelekanga kwabaninzi. Esi sikhokelo sichaza injongo yayo, ukusebenza kwayo, kunye nokusetyenziswa kwayo—nokuba ukwilebhu yebhayoloji yeseli, kwiziko lezonyango, okanye kwiziko lophando lwezonyango. Yintoni i-CO₂ Incubator? I-CO₂ incubator yi-en...Funda ngokugqithisileyo -
Isebenza kangakanani indlela yokubulala iintsholongwane eshushu kakhulu kwi-CO₂ incubator?
Ungcoliseko lwenkcubeko yeseli ludla ngokuba yingxaki edla ngokufunyanwa kwiilebhu zenkcubeko yeseli, ngamanye amaxesha enemiphumo emibi kakhulu. Ungcoliseko lwenkcubeko yeseli lunokwahlulwa lube ziindidi ezimbini eziphambili, ungcoliseko lweekhemikhali ezifana nokungcola okukwimidiya, i-serum namanzi, ii-endotoxins, iip...Funda ngokugqithisileyo -
Kutheni i-CO2 incubator yam inomswakama?
Xa sisebenzisa i-CO2 incubator ukukhulisa iiseli, ngenxa yomahluko kubungakanani bolwelo olongeziweyo kunye nomjikelo wenkcubeko, sineemfuno ezahlukeneyo zomswakama ohambelanayo kwi-incubator. Kwiimvavanyo kusetyenziswa iipleyiti zenkcubeko zeeseli ezingama-96 ezinomjikelo wenkcubeko omde, ngenxa ye-amo encinci...Funda ngokugqithisileyo -
Ungayikhetha njani i-amplitude efanelekileyo ye-incubator eshukumayo?
Ithini i-amplitude ye-shaker? I-amplitude ye-incubator shaker bububanzi be-pallet ejikelezayo, ngamanye amaxesha ebizwa ngokuba yi-"oscillation diameter" okanye "track diameter": Ø. I-Radobio inikezela ngee-shakers ezisemgangathweni ezine-amplitude ze-3mm, 25mm, 26mm kunye ne-50mm,...Funda ngokugqithisileyo -
Yintoni umahluko phakathi kwenkcubeko yeeseli ezinamathelayo kunye nenkcubeko yeeseli ezixhonyiweyo?
Uninzi lweeseli ezivela kwizilwanyana ezinethambo lomqolo, ngaphandle kweeseli ze-hematopoietic kunye nezinye iiseli ezimbalwa, zixhomekeke ekunamatheleni kwaye kufuneka zikhuliswe kwindawo efanelekileyo enyangiwe ngokukodwa ukuvumela ukunamathela kweseli nokusasaza. Nangona kunjalo, iiseli ezininzi zikwafanelekile ukukhuliswa kokuxhonywa....Funda ngokugqithisileyo -
Yintoni umahluko phakathi kwe-TC sensor kunye ne-IR sensor kwi-CO₂ incubator?
Xa kukhuliswa ii-cell cultures, ukuqinisekisa ukukhula okufanelekileyo, ubushushu, ukufuma, kunye namanqanaba e-CO2 kufuneka kulawulwe. Amanqanaba e-CO2 abalulekile kuba anceda ukulawula i-pH yendawo yokukhulisa izityalo. Ukuba kukho i-CO2 eninzi kakhulu, iya kuba ne-asidi kakhulu. Ukuba akukho...Funda ngokugqithisileyo -
Kutheni usebenzisa i-CO2 incubator ukuze kukhule iiseli?
I-pH yesisombululo esiqhelekileyo senkcubeko yeseli iphakathi kwe-7.0 kunye ne-7.4. Ekubeni inkqubo ye-carbonate pH buffer iyinkqubo ye-physiological pH buffer (yinkqubo ebalulekileyo ye-pH buffer egazini lomntu), isetyenziselwa ukugcina i-pH ezinzileyo kwiinkcubeko ezininzi. Inani elithile le-sodium bicarbonate lidla ngokufuna ...Funda ngokugqithisileyo -
Impembelelo yokutshintsha kobushushu kwinkcubeko yeseli kwi-CO2 incubator
Ubushushu buyiparameter ebalulekileyo kwinkcubeko yeseli kuba buchaphazela ukuphinda-phinda kweziphumo. Utshintsho lobushushu olungaphezulu okanye olungaphantsi kwama-37°C lunempembelelo ebaluleke kakhulu kwi-kinetics yokukhula kweseli yeeseli zezilwanyana ezincancisayo, ezifana nezo zeeseli zebhaktheriya. Utshintsho kwimbonakalo yejini kunye ...Funda ngokugqithisileyo -
Ukusetyenziswa kwe-shaking incubator kwinkcubeko yeeseli zebhayoloji
Inkcubeko yebhayoloji yahlulwe yaba yinkcubeko engashukumiyo kunye nenkcubeko yokushukuma. Inkcubeko yokushukuma, eyaziwa ngokuba yinkcubeko yokuxhoma, yindlela yokukhulisa apho iiseli ze-microbial zifakwa khona kwi-medium engamanzi kwaye zibekwe kwi-shaker okanye kwi-oscillator ukuze ziqhubeke zishukuma. Isetyenziswa kakhulu kwi-strain screeni...Funda ngokugqithisileyo
