Hoʻopuka ka incubator CO2 i ka condensation, ʻoi aku ka kiʻekiʻe o ka haʻahaʻa pili?
Ke hoʻohana mākou i ka incubator CO2 e hoʻoulu i nā cell, ma muli o ka ʻokoʻa o ka nui o ka wai i hoʻohui ʻia a me ka pōʻai moʻomeheu, loaʻa iā mākou nā koi like ʻole no ka haʻahaʻa pili i ka incubator.
No nā hoʻokolohua e hoʻohana ana i nā papa moʻomeheu 96-well me kahi pōʻai moʻomeheu lōʻihi, ma muli o ka liʻiliʻi o ka wai i hoʻohui ʻia i ka luawai hoʻokahi, aia ka pilikia e maloʻo ka hopena moʻomeheu inā e hoʻoheheʻe ʻia no ka manawa lōʻihi ma 37 ℃.
Higher pili humidity i loko o ka incubator, no ka laʻana, e hiki oi aku mamua o 90%, hiki pono e hoemi i ka evaporation o ka wai, eia nae, he pilikia hou ua ala mai, he nui cell moʻomeheu experimentalists ua ike i ka incubator mea oluolu e hana condensate i kiʻekiʻe humidity kūlana, condensate hana ina uncontrolled, e ohi a oi aku, i kekahi pilikia o ka maʻi bacteria i lawe mai i ka maʻi bacteria.
No laila, ʻo ka hanauna o ka condensation i loko o ka incubator no ka mea kiʻekiʻe loa ka haʻahaʻa pili?
ʻO ka mea mua, pono mākou e hoʻomaopopo i ka manaʻo o ka haʻahaʻa pili,ka haʻahaʻa pili (Relative Humidity, RH)ʻo ia ka ʻike maoli o ka mahu wai ma ka lewa a me ka pākēneka o ka mahu wai ma ka saturation ma ka mahana like. Hōʻike ʻia ma ke ʻano:
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ʻo ka pākēneka o ka haʻahaʻa pili e hōʻike ana i ka ratio o ka maʻiʻo wai i loko o ka lewa i ka maʻiʻo hiki loa.
kikoʻī:
* 0% RH:ʻAʻohe mahu wai ma ka lewa.
* 100% RH:Hoʻopiha ʻia ka ea i ka mahu wai a ʻaʻole hiki ke paʻa i ka mahu wai hou aʻe ka condensation.
* 50% RH:Hōʻike i ka nui o ka mahu wai i kēia manawa ma ka lewa he hapalua ia o ka nui o ka mahu wai maʻemaʻe i kēlā mahana. Inā he 37°C ka mahana, a laila ma kahi o 6.27 kPa ke kaomi mahu. No laila, ʻo ke kaomi mahu wai ma 50% haʻahaʻa pili ma kahi o 3.135 kPa.
Pumi mahu wai pihaʻo ia ke kaomi i hana ʻia e ka mahu i loko o ka māhele kinoea i ka wā e noho ai ka wai wai a me kāna mahu i loko o ka equilibrium hoʻoikaika i kekahi mahana.
ʻO ka mea kikoʻī, i ka wā e noho pū ai ka mahu wai a me ka wai wai i loko o kahi ʻōnaehana paʻa (e laʻa, kahi incubator Radobio CO2 i hoʻopaʻa maikaʻi ʻia), e hoʻomau ka hoʻololi ʻana o nā molekole wai mai ke kūlana wai i ke kūlana kinoea (evaporation) i ka manawa, ʻoiai e hoʻomau ʻia nā molekole wai kinoea i ke kūlana wai (condensation).
I kekahi manawa, ua like ka nui o ka evaporation a me ka condensation, a ʻo ke kaomi mahu ma ia wahi ʻo ia ke kaomi mahu wai piha. Hōʻike ʻia e
1. ʻano kaulike hoʻoikaika kino:ke noho pū ka wai a me ka mahu wai i loko o kahi ʻōnaehana pani, evaporation a condensation a hiki i ke kaulike, ʻaʻole e loli hou ke kaomi o ka mahu wai i loko o ka ʻōnaehana, i kēia manawa ua hoʻopiha ʻia ke kaomi ʻana i ke kaomi wai.
2. pili ana i ka wela:hoʻololi ke kaomi mahu wai piha me ka mahana. Ke piʻi ka mahana, piʻi ka ikehu kinetic o nā molekele wai, hiki i nā molekele wai ke pakele i ke kaʻina kinoea, no laila e piʻi aʻe ke kaomi mahu wai piha. ʻO ka mea ʻē aʻe, i ka emi ʻana o ka mahana, e emi ana ke kaomi mahu wai maʻemaʻe.
3. Nā ʻano:ʻO ke kaomi wai saturated he ʻano hiʻona maʻemaʻe, ʻaʻole hilinaʻi i ka nui o ka wai, me ka mahana wale nō.
ʻO ke kumu hoʻohālike maʻamau i hoʻohana ʻia no ka helu ʻana i ke kaomi mahu wai saturated ka hoʻohālikelike Antoine:
No ka wai, loaʻa nā waiwai like ʻole o ka Antoine mau no nā pae wela like ʻole. ʻO kahi pūʻulu maʻamau o nā mea mau:
* A=8.07131
* B=1730.63
* C=233.426
Pili kēia pūʻulu o nā mea mau i ka pae wela mai 1°C a 100°C.
Hiki iā mākou ke hoʻohana i kēia mau mea mau no ka helu ʻana i ke kaomi wai piha ma 37°C he 6.27 kPa.
No laila, ehia ka nui o ka wai i loko o ka lewa ma 37 degere Celsius (°C) ma ke ʻano o ke kaomi mahu wai piha?
No ka helu ʻana i ka maʻiʻo nui o ka mahu wai piha (ka haʻahaʻa piha), hiki iā mākou ke hoʻohana i ka hoʻohālikelike Clausius-Clapeyron:
ʻO ke kaomi mahu wai piha: Ma 37°C, he 6.27 kPa ke kaomi mahu.
E hoʻololi ana i ka mahana i Kelvin: T=37+273.15=310.15 K
Hoʻololi i ke ʻano:
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ʻO ka hopena i loaʻa ma ka helu ʻana ma kahi o 44.6 g/m³.
Ma kahi o 37°C, ʻo ka nui o ka mahu wai (ka haʻahaʻa piha) i ka saturation ma kahi o 44.6 g/m³. 'O ia ho'i, hiki i kēlā me kēia mika cubic o ka ea ke ho'opa'a i 44.6 kalama o ka mahu wai.
ʻO kahi incubator 180L CO2 e paʻa wale ma kahi o 8 grams o ka mahu wai.Ke hoʻopiha ʻia ka pā humidification a me nā moku moʻomeheu i nā wai, hiki ke maʻalahi ka haʻahaʻa pili i nā waiwai kiʻekiʻe, kokoke i nā waiwai haʻahaʻa saturation.
Ke piʻi ka haʻahaʻa pili i 100%,hoʻomaka ka mahu wai e hoʻohāinu. I kēia manawa, hiki i ka nui o ka mahu wai i ka lewa i ka waiwai kiʻekiʻe e hiki ke paʻa i ka mahana o kēia manawa, ʻo ia hoʻi ka saturation. ʻO ka hoʻonui hou ʻana i ka mahu wai a i ʻole ka emi ʻana o ka mahana e hoʻoheheʻe ai ka mahu wai i wai wai.
Hiki nō hoʻi ka condensation ke ʻoi aku ka haʻahaʻa haʻahaʻa ma mua o 95%,akā, pili kēia i nā mea ʻē aʻe e like me ka mahana, ka nui o ka mahu wai i ka lewa, a me ka wela o ka ʻili. ʻO kēia mau mea hoʻoikaika e like me kēia:
1. Hoemi i ka wela:Ke kokoke ka nui o ka mahu wai ma ka lewa i ka saturation, hiki i ka hoʻemi iki ʻana o ka mahana a i ʻole ka piʻi ʻana o ka nui o ka mahu wai e hoʻomaka ai ka condensation. No ka laʻana, i ka wela fluctuations i loko o ka incubator hiki ke alakai i ka hanauna o ka condensate, no laila, i ka mahana o ka oi paʻa incubator e loaʻa i ka inhibitory hopena i ka hanauna o condensate.
2. ka wela o ka ili kūloko ma lalo o ka mahana o ka hau:ʻO ka wela o ka ili kūloko he haʻahaʻa ma mua o ka mahana o ka hau, e hoʻoheheʻe ʻia ka mahu wai i loko o nā droplets wai ma luna o kēia mau papa, no laila e ʻoi aku ka maikaʻi o ka hoʻohuihui wela o ka incubator i ka inhibition o ka condensation.
3. Hoʻonui ʻia ka mahu wai:no ka laʻana, humidification pan a me ka moʻomeheu ipu me ka nui nui o ka wai, a me ka incubator ua oi aku ka sila, i ka nui o ka wai mahu ma ka lewa i loko o ka incubator mahuahua ma mua o kona hiki ke loaʻa i ka mahana wela o kēia manawa, ʻoiai inā e hoʻololi ʻole ka mahana, e hana ʻia ka condensation.
No laila, ʻo kahi incubator CO2 me ka hoʻomalu wela maikaʻi loa he hopena inhibiting i ka hanauna o ka condensate, akā inā ʻoi aku ka haʻahaʻa haʻahaʻa ma mua o 95% a hiki i ka saturation, e piʻi nui ka hiki o ka condensation.no laila, i ka wā e hoʻoulu ai mākou i nā cell, ma waho aʻe o ke koho ʻana i kahi incubator CO2 maikaʻi, pono mākou e hoʻāʻo e pale i ka pilikia o ka condensation i lawe ʻia e ka ʻimi ʻana i ka haʻahaʻa kiʻekiʻe.
Ka manawa hoʻouna: Iulai-23-2024