Gịnị bụ cell omenala nkwusioru vs adherent?
Ọtụtụ mkpụrụ ndụ sitere na vertebrates, ma ewezuga mkpụrụ ndụ hematopoietic na mkpụrụ ndụ ole na ole ndị ọzọ, na-adabere na ya, a ga-akụkwa ya na mkpụrụ kwesịrị ekwesị nke edoziworo kpọmkwem iji kwe ka nrapado na gbasaa cell. Agbanyeghị, ọtụtụ sel dịkwa mma maka omenala nkwusioru. N'otu aka ahụ, ọtụtụ mkpụrụ ndụ ụmụ ahụhụ dị na-ere ahịa na-eto nke ọma na omenala na-agbaso ma ọ bụ nkwusioru.
Enwere ike idobe mkpụrụ ndụ nkwusioru-omenala na flask omenala ndị a na-agwọbeghị maka ọdịbendị anụ ahụ, mana ka ụda olu na elu nke ọdịbendị na-abawanye, a na-egbochi mgbanwe gas zuru oke ma ọ dị mkpa ka ọ kpasuo ya iwe. A na-enweta mkpasu iwe a site na ihe ndọta ma ọ bụ erlenmeyer flask na incubator na-ama jijiji.
| Omenala Adherent  | Omenala nkwusioru  |
| Kwesịrị ekwesị maka ọtụtụ ụdị cell, gụnyere omenala sel mbụ | Kwesịrị ekwesị maka sel nwere ike ịmebe nkwusioru yana ụfọdụ sel ndị ọzọ na-adịghị agbaso (dịka ọmụmaatụ, mkpụrụ ndụ hematopoietic) |
| Na-achọ subculture nke oge, mana enwere ike inyocha ya ngwa ngwa n'okpuru microscope tụgharịrị | Ọ dị mfe na subculture, ma na-achọ ọnụ ọgụgụ sel kwa ụbọchị na nyocha ike iji hụ uto; omenala nwere ike diluted iji kpalie uto |
| Selụ na-enzymatically (dịka trypsin) ma ọ bụ mebere nkewa | Ọ dịghị enzymatic ma ọ bụ n'ibu dissociation chọrọ |
| Uto na-ejedebe site na mpaghara elu, nke nwere ike igbochi mmepụta mmepụta | Uto na-ejedebe site na ntinye nke mkpụrụ ndụ na ọkara, yabụ enwere ike ịbawanye ngwa ngwa |
| Ọgbọ omenala cell chọrọ ọgwụgwọ elu anụ ahụ | Enwere ike idowe ya n'ime arịa omenala na-enweghị ọgwụgwọ elu anụ ahụ, mana chọrọ mkpasu iwe (ya bụ, ịma jijiji ma ọ bụ ịkpali) maka mgbanwe gas zuru oke. |
| Ejiri ya maka cytology, nchịkọta cell na-aga n'ihu na ọtụtụ ngwa nyocha | Ejiri ya maka mmepụta protein buru ibu, nchịkọta batch cell na ọtụtụ ngwa nyocha |
| Nweta incubator CO2 gị na efere omenala cell ugbu a:C180 140°C Okpomọkụ sterilization CO2 IncubatorEfere Omenala Cell | Nweta CO2 incubator shaker na erlenmeyer flasks ugbu a: |
Oge nzipu: Ọgọst-28-2023