Yintoni ukumiswa kwenkcubeko yeseli xa kuthelekiswa nokunamathela?
Uninzi lweeseli ezivela kwizilwanyana ezinethambo lomqolo, ngaphandle kweeseli ze-hematopoietic kunye nezinye iiseli ezimbalwa, zixhomekeke kwi-namento kwaye kufuneka zikhuliswe kwindawo efanelekileyo enyangiwe ngokukodwa ukuvumela ukunamathela kweseli kunye nokusasazwa. Nangona kunjalo, iiseli ezininzi zikwafanelekile ukukhulisa i-suspension. Ngokufanayo, uninzi lweeseli zezinambuzane ezithengiswayo zikhula kakuhle kwi-namento okanye kwi-suspension culture.
Iiseli ezikhuliswe nge-suspension zingagcinwa kwiiflaski ze-culture ezingakhange zinyangwe ukuze kukhuliswe izicubu, kodwa njengoko umthamo kunye nommandla womphezulu we-culture usanda, ukutshintshiselana kwegesi okwaneleyo kuyathintelwa kwaye i-medium kufuneka ishukunyiswe. Oku kuvuswa kudla ngokufezekiswa nge-magnetic stirrer okanye iflask ye-erlenmeyer kwi-incubator eshukumayo.
| Inkcubeko yoBuchule  | Inkcubeko Yokumiswa  |
| Ifanelekile kwiintlobo ezininzi zeeseli, kuquka nokukhula kweeseli eziphambili | Ezilungele iiseli zinokukhuliswa ngokumiswa kunye nezinye iiseli ezingabambeleliyo (umz., iiseli ze-hematopoietic) |
| Ifuna i-subculture yexesha elithile, kodwa inokuhlolwa ngokulula ngokubonakalayo phantsi kwe-microscope eguqulweyo | Kulula ukuyikhulisa kancinci, kodwa ifuna inani leeseli zemihla ngemihla kunye novavanyo lokuphila ukuze kubonwe ukukhula; iinkcubeko zinokuncitshiswa ukuze kukhuthazwe ukukhula |
| Iiseli zi-enzyme (umz. i-trypsin) okanye zahlulwe ngoomatshini | Akukho mfuneko yokwahlukana kwe-enzyme okanye i-mechanical |
| Ukukhula kunqunyelwe bubungakanani bomhlaba, nto leyo enokunciphisa isivuno semveliso | Ukukhula kunqunyelwe kukuxinana kweeseli kwindawo ephakathi, ngoko ke kunokwandiswa ngokulula |
| Imithambo yokukhulisa iiseli efuna unyango lomphezulu wokukhulisa iithishu | Ingagcinwa kwimithambo yegazi ngaphandle konyango lokukhulisa izicubu, kodwa ifuna ukushukunyiswa (oko kukuthi, ukushukunyiswa okanye ukuxutywa) ukuze kutshintshiselwe igesi ngokwaneleyo |
| Isetyenziselwa i-cytology, ukuqokelelwa kweeseli okuqhubekayo kunye nezicelo ezininzi zophando | Isetyenziselwa ukuvelisa iiproteni ezininzi, ukuqokelelwa kweeseli zebatch kunye nezicelo ezininzi zophando |
| Fumana i-CO2 incubator yakho kunye neeplate zenkcubeko yeeseli ngoku:I-C180 140°C I-High Heat Sterilization CO2 IncubatorIpleyiti Yenkcubeko Yeseli | Zifumanele i-CO2 incubator shaker kunye neeflasks ze-erlenmeyer ngoku: |
Ixesha lokuthumela: Agasti-28-2023