Kodi kuimitsidwa kwa chikhalidwe cha maselo ndi chiyani poyerekeza ndi kutsamira?
Maselo ambiri ochokera ku zamoyo zokhala ndi msana, kupatula maselo a hematopoietic ndi maselo ena ochepa, amadalira kwambiri kutsata ndipo ayenera kulimidwa pa nthaka yoyenera yomwe yathandizidwa kuti maselo azigwirizana ndikufalikira. Komabe, maselo ambiri ndi oyeneranso kukulitsa kutsamira. Mofananamo, maselo ambiri a tizilombo omwe amapezeka m'masitolo amakula bwino mu kutsamira kapena kutsamira.
Maselo opangidwa ndi suspension-cultured cells amatha kusungidwa m'mabotolo opangidwa ndi suspension omwe sanachiritsidwe kuti apangidwe ndi minofu, koma pamene kuchuluka ndi malo opangidwa ndi suspension akuwonjezeka, kusinthana kwa mpweya kokwanira kumalephereka ndipo malo opangidwa ndi suspension amafunika kusunthidwa. Kusunthika kumeneku nthawi zambiri kumachitika ndi magnetic stirrer kapena erlenmeyer flask mu shaking incubator.
| Chikhalidwe Chotsatira  | Chikhalidwe Choyimitsidwa  |
| Yoyenera mitundu yambiri ya maselo, kuphatikizapo kukula kwa maselo oyambira | Oyenera maselo akhoza kukulitsidwa ndi kuimitsidwa ndi maselo ena osagwira ntchito (monga maselo a hematopoietic) |
| Zimafuna kubzala nthawi ndi nthawi, koma zimatha kuwonedwa mosavuta pogwiritsa ntchito maikulosikopu yozungulira | Kulima pang'ono n'kosavuta, koma kumafuna kuchuluka kwa maselo tsiku ndi tsiku ndi kuyesa momwe angakulire; kubzala kungachepe kuti kulimbikitse kukula. |
| Maselo amapangidwa ndi enzyme (monga trypsin) kapena amalekanitsidwa ndi makina | Palibe kugawanika kwa enzyme kapena makina komwe kumafunika |
| Kukula kumachepetsedwa ndi malo ozungulira, zomwe zingachepetse zokolola | Kukula kumachepa ndi kuchuluka kwa maselo m'thupi, kotero kumatha kukulitsidwa mosavuta |
| Mitsempha yokulitsa maselo yomwe imafuna chithandizo cha pamwamba pa minofu | Zitha kusungidwa m'mitsempha ya zomera popanda kuchiza pamwamba pa minofu, koma zimafunika kusunthidwa (monga kugwedezeka kapena kusunthidwa) kuti mpweya usinthe bwino. |
| Amagwiritsidwa ntchito pa cytology, kusonkhanitsa maselo mosalekeza komanso ntchito zambiri zofufuza | Amagwiritsidwa ntchito popanga mapuloteni ambiri, kusonkhanitsa maselo a batch ndi ntchito zambiri zofufuza |
| Pezani chosungira chanu cha CO2 ndi mbale zosungira maselo tsopano:Chotengera cha CO2 Chotenthetsera Kutentha Kwambiri cha C180 140°CMbale Yopangira Chikhalidwe cha Cell | Pezani chotsukira cha CO2 incubator ndi ma flask a erlenmeyer tsopano: |
Nthawi yotumizira: Ogasiti-28-2023