Kodi kuyimitsidwa kwa chikhalidwe cha ma cell ndi kutsata ndi chiyani?
Maselo ambiri ochokera ku zinyama zokhala ndi vertebrates, kupatulapo maselo a hematopoietic ndi maselo ena ochepa, amadalira motsatira ndipo amayenera kukulitsidwa pa gawo lapansi loyenera lomwe lakhala likugwiritsidwa ntchito kuti lilole kumatira kwa selo ndi kufalikira. Komabe, maselo ambiri ndi oyenera kuyimitsidwa chikhalidwe. Mofananamo, maselo ambiri a tizilombo omwe amapezeka pamalonda amakula bwino mu chikhalidwe chotsatira kapena kuyimitsidwa.
Maselo oyimitsidwa-oyimitsidwa amatha kusungidwa mu ma flasks a chikhalidwe omwe sanagwiritsidwepo chifukwa cha chikhalidwe cha minofu, koma pamene voliyumu ndi pamwamba pa chikhalidwecho chikuwonjezeka, kusinthana kwa mpweya wokwanira kumalephereka ndipo sing'anga iyenera kugwedezeka. Chisokonezochi nthawi zambiri chimatheka ndi magnetic stirrer kapena erlenmeyer botolo pogwedeza chofungatira.
| Adherent Culture  | Kuyimitsidwa Chikhalidwe  |
| Oyenera mitundu yambiri ya ma cell, kuphatikiza chikhalidwe choyambirira cha cell | Oyenera ma cell amatha kuyimitsidwa otukuka komanso ma cell ena osatsatira (mwachitsanzo, ma cell a hematopoietic) |
| Imafunika nthawi ndi nthawi, koma imatha kuyang'aniridwa mosavuta ndi maikulosikopu | Chosavuta ku subculture, koma chimafunikira kuchuluka kwa maselo atsiku ndi tsiku ndikuyesa kutheka kuti muwone kukula; zikhalidwe zitha kuchepetsedwa kuti zilimbikitse kukula |
| Maselo amapangidwa ndi enzymatic (mwachitsanzo trypsin) kapena amasiyanitsidwa ndi makina | Palibe enzymatic kapena makina dissociation chofunika |
| Kukula kumachepa ndi malo, zomwe zingachepetse zokolola | Kukula kumakhala kochepa chifukwa cha kuchuluka kwa maselo m'kati mwake, kotero akhoza kuchepetsedwa mosavuta |
| Ziwiya zamtundu wa cell zomwe zimafunikira chithandizo chamtundu wa minofu | Itha kusungidwa m'zotengera zachikhalidwe popanda chithandizo chamtundu wamtundu wamtundu, koma zimafunikira chipwirikiti (mwachitsanzo, kugwedezeka kapena kugwedezeka) kuti musinthe mpweya wokwanira. |
| Amagwiritsidwa ntchito pa cytology, kusonkhanitsa ma cell mosalekeza komanso ntchito zambiri zofufuza | Amagwiritsidwa ntchito popanga mapuloteni ambiri, kusonkhanitsa maselo a batch ndi ntchito zambiri zofufuza |
| Pezani chofungatira chanu cha CO2 ndi mbale zama cell pano:C180 140°C Kutentha Kwambiri Kutentha Kwambiri Chotsekera CO2 IncubatorCell Culture Plate | Pezani CO2 incubator shaker ndi erlenmeyer flasks tsopano: |
Nthawi yotumiza: Aug-28-2023