chii chinonzi cell culture suspension vs adherent?
Masero mazhinji kubva kune vertebrates, kunze kwehematopoietic masero uye mamwe mashoma masero, anonamatira-anotsamira uye anofanirwa kukwidziridzwa pane yakakodzera substrate iyo yakanyatso kurapwa kubvumira sero kunamatira uye kupararira. Zvisinei, masero akawanda akakodzerawo kumisa tsika. Saizvozvo, masero ezvipembenene anowanikwa mune zvekutengesa anokura zvakanaka mune adherent kana kuturika tsika.
Masero ekusimudzwa-akagadzirwa anogona kuchengetwa mutsika flasks dzisina kurapwa kune tishu tsika, asi sezvo vhoriyamu uye nzvimbo yepamusoro yetsika inowedzera, kukwana gasi kuchinjana kunodzivirirwa uye yepakati inoda kubvongodzwa. Kubvongodza uku kunowanzoitwa nemagnetic stirrer kana erlenmeyer flask mukuzunza incubator.
| Adherent Tsika  | Kumiswa Kwetsika  |
| Inokodzera akawanda maseru marudzi, kusanganisira yekutanga cell tsika | Inokodzera maseru inogona kumiswa yakasimudzwa uye mamwe masero asina- adherent (semuenzaniso, hematopoietic masero) |
| Inoda periodic subculture, asi inogona kutariswa zviri nyore pasi peiyo inverted microscope | Zviri nyore kuita subculture, asi inoda mazuva ese maseru kuverenga uye kugona kuyedza kuona kukura; tsika dzinogona kuderedzwa kuti dzikurudzire kukura |
| Masero ane enzymatic (eg trypsin) kana kuti akaparadzaniswa nemuchina | Hapana enzymatic kana mechanical dissociation inodiwa |
| Kukura kunoganhurirwa nenzvimbo, izvo zvinogona kudzikisira goho rekugadzira | Kukura kunogumira nekusangana kwemaseru ari pakati, saka anogona kukwidziridzwa zviri nyore |
| Sero tsika midziyo inoda tishu tsika pamusoro kurapwa | Inogona kuchengetedzwa mumidziyo yetsika pasina matishu etsika kurapwa kwepamusoro, asi inoda kubvongodzwa (kureva, kuzunungusa kana kubvongodza) kuitira kushandura gasi kwakaringana. |
| Inoshandiswa cytology, inoenderera kuunganidzwa kwesero uye akawanda ekutsvagisa mashandisirwo | Inoshandiswa kugadzirwa kweprotein yakawanda, batch cell collection uye akawanda ekutsvagisa maapplication |
| Tora CO2 incubator yako uye cell tsika mahwendefa izvozvi:C180 140°C High Heat Sterilization CO2 IncubatorSero Culture Plate | Tora iwe CO2 incubator shaker uye erlenmeyer flasks izvozvi: |
Nguva yekutumira: Aug-28-2023