Sei chitofu changu cheCO2 chine condensation?
PatinoshandisaChinotonhorera cheCO2Kuti tikure masero, nekuda kwekusiyana kwehuwandu hwemvura inowedzerwa uye kutenderera kwekukura, tine zvinodiwa zvakasiyana zvehunyoro mu incubator.
Pakuyedza kushandisa maplate e 96-well cell culture ane circuit yakareba ye culture, nekuda kwehuwandu hushoma hwemvura inowedzerwa mu tsime rimwe chete, pane njodzi yekuti culture solution ichaoma kana ikapwa kwenguva yakareba pa37 ℃.
Kuwanda kwehunyoro mu incubator, semuenzaniso, kusvika pamusoro pe90%, kunogona kuderedza kubuda kwemvura mumvura, zvisinei, dambudziko idzva ramuka, vazhinji vanoongorora masero vakaona kuti incubator iri nyore kugadzira condensate mumamiriro ehunyoro hwakanyanya, kugadzirwa kwe condensate kana kusingadzorwi, kuchaunganidzwa zvakanyanya, kune masero emhando yeutachiona kwakaunza njodzi yehutachiona.
Saka, kugadzirwa kwemvura inodziya mu incubator imhaka yekuti hunyoro hwacho hwakanyanya here?
Chekutanga, tinofanira kunzwisisa pfungwa yehunyoro,hunyoro hushoma (Hunyoro hushoma, RH)ndiyo huwandu chaihwo hwemvura inopfungaira mumhepo uye huwandu hwemvura inopfungaira mumhepo pahuwandu hwemvura pakupisa kwakafanana. Inotsanangurwa mufomura:
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huwandu hwehunyoro hunomiririra chiyero chehuwandu hwemvura mumhepo kusvika pahuwandu hwakanyanya hunogoneka.
Zvikuru sei:
* 0% RH:Hakuna mhute yemvura mumhepo.
* 100% RH:Mhepo inenge yazara neutsi hwemvura uye haigone kuchengetedza utsi hwemvura hwakawanda uye huchaoma.
* 50% RH:Zvinoratidza kuti huwandu hwemvura iri mumhepo huripo parizvino hunosvika hafu yehuwandu hwemvura yakasviba patembiricha iyoyo. Kana tembiricha iri 37°C, zvinoreva kuti kumanikidzwa kwemvura yakasviba kunenge 6.27 kPa. Saka, kumanikidzwa kwemvura pa50% hunyoro hunenge 3.135 kPa.
Kumanikidzwa kwemvura ine utsi hwakazaraidzvinyiriro inogadzirwa neutsi muchikamu chegasi kana mvura yemvura neutsi hwayo zviri mu dynamic equilibrium pane imwe tembiricha.
Zvikuru sei, kana mvura yakasviba nemvura yemvura zvikabatana muhurongwa hwakavharwa (semuenzaniso, incubator yeRadobio CO2 yakavharwa zvakanaka), mamorekuru emvura acharamba achichinja kubva pamamiriro emvura kuenda kumamiriro egasi (evaporation) nekufamba kwenguva, ukuwo mamorekuru emvura yegasi acharamba achichinja kuenda kumamiriro emvura (condensation).
Pane imwe nzvimbo, mwero wekubuda kwemvura uye kuumbwa kwemvura kwakaenzana, uye kumanikidzwa kwemhepo panguva iyoyo isimba remvura yakasviba. Inoratidzwa ne
1. kuenzana kwesimba:Kana mvura nemweya wemvura zvikabatana muchimiro chakavharwa, kupwanyika uye kuungana kuti zvisvike pakuenzana, kumanikidzwa kwemweya wemvura muchimiro hakuchachinji, panguva ino kumanikidzwa kwacho kwakazara nekumanikidzwa kwemweya wemvura.
2. kutsamira pakupisa:Kumanikidzwa kwemvura ine mhute yakaguta kunochinja nekupisa. Kana tembiricha ikawedzera, simba remagetsi remamorekuru emvura rinowedzera, mamorekuru emvura akawanda anogona kubuda achienda kuchikamu chegasi, saka kumanikidzwa kwemhute yemvura ine mhute kunowedzera. Kusiyana neizvi, kana tembiricha ikadzikira, kumanikidzwa kwemhute yemvura ine mhute yakaguta kunoderera.
3. Hunhu:Kumanikidzwa kwemvura yakasviba chinhu chinoratidza zvinhu chete, hazvitsamiri pahuwandu hwemvura, asi chete nekupisa.
Fomura yakajairika inoshandiswa kuverenga kumanikidzwa kwemvura yakasviba ndiyo equation yeAntoine:
Pamvura, chinhu chinonzi Antoine constant chine mavalues akasiyana pamhando dzakasiyana dzetembiricha. Seti yezvikamu zvakafanana ndeiyi:
* A=8.07131
* B=1730.63
* C=233.426
Seti iyi yema "constants" inoshanda pakupisa kubva pa1°C kusvika 100°C.
Tinogona kushandisa ma constant aya kuverenga kuti kumanikidzwa kwemvura yakasviba pa37°C i6.27 kPa.
Saka, mvura yakawanda sei iri mumhepo pa37 degrees Celsius (°C) mumamiriro ekumanikidzwa kwemvura yakasviba?
Kuti tiverenge huwandu hwemvura yakasviba (hunyoro hwakakwana), tinogona kushandisa fomura yeClausius-Clapeyron equation:
Kumanikidzwa kwemvura yakaguta: Pa37°C, kumanikidzwa kwemvura yakaguta i6.27 kPa.
Kushandura tembiricha kuita Kelvin: T=37+273.15=310.15 K
Kutsiva mufomura:
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mhedzisiro inowanikwa nekuverenga inenge 44.6 g/m³.
Pa37°C, huwandu hwemvura inopfungaira (hunyoro hwakakwana) pahuwandu hwe44.6 g/m³. Izvi zvinoreva kuti cubic mita yega yega yemhepo inogona kutakura magiramu 44.6 emvura inopfungaira.
Chinotonhodza cheCO2 che180L chinongobata magiramu masere chete emvura inopfungaira.Kana pani yekunyorovesa mvura pamwe chete nemidziyo yekurera mvura zvikazadzwa nemvura, hunyoro hunogona kusvika padanho repamusoro, kunyangwe padanho rehuwandu hwehunyoro.
Kana hunyoro hwasvika 100%,mhute yemvura inotanga kuungana. Panguva iyi, huwandu hwemhute yemvura mumhepo hunosvika pahuwandu hwainogona kubata patembiricha yazvino, kureva kuwanda. Kuwedzera kwemhute yemvura kana kudzikira kwetembiricha kunoita kuti mhute yemvura iungane kuita mvura yemvura.
Kunyorova kunogonawo kuitika kana hunyoro huchipfuura 95%,asi izvi zvinoenderana nezvimwe zvinhu zvakaita sekupisa, huwandu hwemvura iri mumhepo, uye kupisa kwepamusoro. Zvinhu izvi zvinopesvedzera zvinotevera:
1. Kudzikira kwekupisa:Kana huwandu hwemvura mumhepo hwava pedyo nekuzara, kudzikira kudiki kwekupisa kana kuwedzera kwehuwandu hwemvura kunogona kukonzera kuungana kwemvura. Semuenzaniso, kuchinja-chinja kwekupisa mu incubator kunogona kutungamira mukuumbwa kwe condensate, saka tembiricha yacho yakadzikama zvikuru ichava nemhedzisiro inodzivisa kugadzirwa kwe condensate.
2. tembiricha yemunzvimbo iri pasi petembiricha yedova:Kana tembiricha yenzvimbo yacho yakaderera pane tembiricha yedova, utsi hwemvura huchapinda mudutu remvura panzvimbo idzi, saka tembiricha yakafanana yeincubator ichaita zviri nani mukudzivirira kupfava.
3. Kuwedzera kwemvura inopfungaira:semuenzaniso, midziyo yekubikisa humidification uye midziyo yekurima ine huwandu hwakawanda hwemvura, uye incubator zvinovharwa zviri nani, kana huwandu hwemvura mumhepo iri mukati me incubator hukawedzera kupfuura kugona kwayo kwepamusoro patembiricha iripo, kunyangwe kana tembiricha ikaramba isina kuchinja, condensation ichagadzirwa.
Saka, chitofu cheCO2 chine tembiricha yakanaka chinodzivisa kugadzirwa kwemvura inodziya, asi kana hunyoro hwacho hukadarika 95% kana kutosvika pakuzara, mukana wekuti mvura inodziya unowedzera zvakanyanya.saka, patinorima masero, pamusoro pekusarudza CO2 incubator yakanaka, tinofanira kuedza kudzivirira njodzi yekuoma kwemvura kunokonzerwa nekutsvaga hunyoro hwakawanda.
Nguva yekutumira: Chikunguru-23-2024