Impembelelo yokutshintsha kobushushu kwinkcubeko yeeseli
Iqondo lobushushu liyiparameter ebalulekileyo kwinkcubeko yeseli kuba ichaphazela ukuveliswa kweziphumo. Ubushushu bokutshintsha ngaphezulu okanye ngaphantsi kwe-37 ° C kunempembelelo ebaluleke kakhulu kwi-kinetics yokukhula kweeseli zeeseli ezincancisayo, ezifana neeseli zebhaktheriya. Utshintsho kwimbonakalo yemfuza kunye nokuguqulwa kwesakhiwo seselula, ukuqhubela phambili komjikelezo weseli, ukuzinza kwe-mRNA kunokubonwa kwiiseli ze-mammalian emva kweyure enye kwi-32ºC. Ukongeza kokuchaphazela ngokuthe ngqo ukukhula kweeseli, utshintsho kwiqondo lokushisa luchaphazela i-pH yeendaba, njengoko i-solubility ye-CO2 iguqula i-pH (i-pH inyuka kumaqondo aphantsi). Iiseli ezanyisayo ezikhulisweyo zinokunyamezela ukuhla kweqondo lobushushu. Zinokugcinwa kwi-4 °C iintsuku eziliqela kwaye ziyakwazi ukunyamezela iqhwa ukuya kwi -196 °C (zisebenzisa iimeko ezifanelekileyo). Nangona kunjalo, azikwazi ukunyamezela amaqondo obushushu angaphezu kwe-2 °C ngaphezu kwesiqhelo ngaphezu kweeyure ezimbalwa kwaye ziya kufa ngokukhawuleza kwi-40 °C nangaphezulu. Ukuqinisekisa ukuphindaphinda okuphezulu kweziphumo, nokuba ngaba iiseli ziphila, kufuneka kuthathelwe ingqalelo ukuze kugcinwe ubushushu obungaguqukiyo ngangokunokwenzeka ngexesha lokufakwa kunye nokuphathwa kweeseli ngaphandle kwe-incubator.
Izizathu zokutshintsha kweqondo lokushisa ngaphakathi kwe-incubator
Uya kuqaphela ukuba xa ucango lwe-incubator luvuliwe, ubushushu behla ngokukhawuleza kwixabiso elimiselweyo lama-37 °C. Ngokubanzi, iqondo lobushushu liya kubuyiselwa emva kwemizuzu embalwa emva kokuba umnyango uvaliwe. Enyanisweni, iinkcubeko ezimileyo zifuna ixesha lokubuyisela kwiqondo lokushisa elimiselweyo kwi-incubator. Inani lezinto ezinokuchaphazela ixesha elithathayo ukuba inkcubeko yeseli iphinde ifumane ubushushu emva konyango ngaphandle kwe-incubator.Le miba ibandakanya:
- ▶ubude bexesha iiseli beziphumile kwi-incubator
- ▶uhlobo lweflaski ezikhuliswa ngayo iiseli (ijometri ichaphazela ukuhanjiswa kobushushu)
- ▶ Inani lezikhongozeli ezikwi-incubator .
- ▶ Ukudibana ngokuthe ngqo kweeflask kunye neshelufu yentsimbi kuchaphazela utshintshiselwano lobushushu kunye nesantya sokufikelela kwelona qondo liphezulu lobushushu, ngoko ke kungcono ukunqanda iiflaski kunye nokubeka inqanawa nganye.
- ▶ ngokuthe ngqo kwishelufa ye-incubator.
Ubushushu bokuqala bazo naziphi na izikhongozeli ezitsha kunye nemithombo yeendaba esetyenzisiweyo iyakuchaphazela ixesha elithathwayo ukuze iiseli zibe kwimeko yazo efanelekileyo; xa ubushushu bawo busezantsi, kokukhona kuthatha ixesha elide.
Ukuba zonke ezi zinto ziyatshintsha ngokuhamba kwexesha, ziya kwandisa nokwahluka phakathi kwemifuniselo. Kuyimfuneko ukunciphisa oku kutshintsha kweqondo lokushisa, nokuba akusoloko kunokwenzeka ukulawula yonke into (ingakumbi ukuba abantu abaninzi basebenzisa i-incubator efanayo).
Indlela yokunciphisa ukuhluka kweqondo lokushisa kunye nokunciphisa ixesha lokubuyisela ubushushu
Ngokufudumeza okuphakathi
Abanye abaphandi baqhele ukufudumeza zonke iibhotile zemidiya kwibhafu yamanzi eyi-37 °C ukubazisa kobu bushushu ngaphambi kokusetyenziswa. Kwakhona kunokwenzeka ukutshisa i-medium kwi-incubator esetyenziselwa kuphela ukufudumala okuphakathi kwaye kungekhona kwinkcubeko yeseli, apho i-medium inokufikelela kubushushu obuphezulu ngaphandle kokuphazamisa iinkcubeko zeseli kwenye i-incubator. Kodwa oku, ngokokwazi kwethu, akusoloko kuyindleko efikelelekayo.
Ngaphakathi kwi Incubator
Vula ucango lwe-incubator kancinci kwaye uluvale ngokukhawuleza. Gwema iindawo ezibandayo, ezidala ukuhluka kweqondo lokushisa kwi-incubator. Shiya isithuba phakathi kweeflaski ukuze umoya ujikeleze. Iishelufa ezingaphakathi kwi-incubator zinokugqojozwa. Oku kuvumela ukusasazwa kobushushu obungcono njengoko kuvumela umoya ukuba udlule kwimingxuma. Nangona kunjalo, ubukho bemingxuma bunokukhokelela ekuhlukeni ekukhuleni kweeseli, kuba kukho ukungafani kobushushu phakathi kwendawo enemingxuma kunye nommandla onemeta. Ngezi zizathu, ukuba iimvavanyo zakho zifuna ukukhula okufanayo kwenkcubeko yeeseli, ungabeka iiflaski zenkcubeko kwiinkxaso zentsimbi ezinemigangatho emincinci yokunxibelelana, edla ngokungafunekiyo kwinkcubeko yesiqhelo yeeseli.
Ukunciphisa Ixesha Lokwenziwa Kweseli
Ukunciphisa ixesha elichithwe kwinkqubo yonyango lweeseli, kufuneka
- ▶Lungelelanisa zonke izinto eziyimfuneko kunye nezixhobo ngaphambi kokuba uqalise ukusebenza.
- ▶Sebenza ngokukhawuleza nangokutyibilikayo, uphonononga iindlela zovavanyo kwangaphambili ukuze imisebenzi yakho iphindaphindeke kwaye izenzekele.
- ▶Nciphisa ukudityaniswa kolwelo ngomoya ophakathi.
- ▶Gcina iqondo lobushushu elingaguqukiyo kwilebhu yenkcubeko yeseli apho usebenza khona.
Ixesha lokuposa: Aug-18-2023