cell culture suspension vs adherent yog dab tsi?
Feem ntau cov hlwb los ntawm vertebrates, tshwj tsis yog cov hematopoietic hlwb thiab ob peb lwm cov hlwb, yog adherent-dependent thiab yuav tsum tau coj mus rau ib tug tsim nyog substrate uas tau tshwj xeeb kho mob kom cia cell adhesion thiab kis tau. Txawm li cas los xij, ntau lub hlwb kuj tsim nyog rau kev ncua kev coj noj coj ua. Ib yam li ntawd, feem ntau cov kab kab uas muaj nyob rau hauv kev lag luam loj hlob zoo nyob rau hauv ob qho tib si adherent los yog ncua kab lis kev cai.
Kev ncua-cultured hlwb tuaj yeem khaws cia rau hauv kab lis kev cai flasks uas tsis tau kho rau cov kab lis kev cai ntawm cov ntaub so ntswg, tab sis raws li qhov ntim thiab thaj tsam ntawm kab lis kev cai nce ntxiv, kev sib pauv roj txaus raug cuam tshuam thiab qhov nruab nrab yuav tsum muaj kev ntxhov siab. Qhov kev ntxhov siab no feem ntau yog ua tiav los ntawm lub tshuab nqus hlau nplaum lossis lub khob erlenmeyer hauv kev co incubator.
| Adherent Culture  | Suspension Culture  |
| Haum rau feem ntau hom cell, suav nrog cov kab lis kev cai tseem ceeb ntawm tes | Tsim nyog rau cov hlwb tuaj yeem raug tshem tawm kab lis kev cai thiab qee lwm cov hlwb tsis ua raws (xws li, cov hlwb hematopoietic) |
| Yuav tsum tau ncua sij hawm subculture, tab sis tuaj yeem pom tau yooj yim pom nyob rau hauv ib qho inverted microscope | Yooj yim rau subculture, tab sis yuav tsum tau suav cov xov tooj ntawm tes txhua hnub thiab kev ntsuas kev muaj peev xwm los soj ntsuam kev loj hlob; kab lis kev cai yuav diluted los txhawb kev loj hlob |
| Cells yog enzymatically (xws li trypsin) los yog mechanically dissociated | Tsis tas yuav enzymatic lossis mechanical dissociation |
| Kev loj hlob yog txwv los ntawm thaj tsam ntawm thaj chaw, uas tuaj yeem txwv cov qoob loo ntau lawm | Kev loj hlob yog txwv los ntawm cov concentration ntawm cov hlwb hauv nruab nrab, yog li tuaj yeem ua tau yooj yim scaled |
| Cell kab lis kev cai hlab ntsha xav tau cov ntaub so ntswg kab lis kev cai nto kho | tuaj yeem khaws cia hauv kab lis kev cai cov hlab ntsha yam tsis muaj cov ntaub so ntswg kab lis kev cai saum npoo, tab sis yuav tsum muaj kev ntxhov siab (piv txwv li, co lossis nplawm) rau kev sib pauv roj txaus |
| Siv rau cytology, nruam kev sau ntawm tes thiab ntau daim ntawv thov kev tshawb fawb | Siv rau kev tsim cov protein ntau, batch cell sau thiab ntau daim ntawv thov kev tshawb fawb |
| Tau txais koj CO2 incubator thiab cell culture daim hlau tam sim no:C180 140 ° C Kub Kub Sterilization CO2 IncubatorCell Culture Phaj | Tau txais koj CO2 incubator shaker thiab erlenmeyer flasks tam sim no: |
Post lub sij hawm: Jan-03-2024