Uyini umehluko phakathi kokukhuliswa kwamaseli anamathelayo kanye nokukhuliswa kwamaseli amiswe?
Iningi lamaseli avela ezilwaneni ezinomgogodla, ngaphandle kwamaseli e-hematopoietic kanye namanye amaseli ambalwa, ancike ekunamatheleni futhi kumele akhuliswe endaweni efanelekile elashwe ngokukhethekile ukuze kuvunyelwe ukunamathelana nokusabalala kwamaseli. Kodwa-ke, amaseli amaningi nawo afanelekela ukukhuliswa kokulengiswa. Ngokufanayo, iningi lamaseli ezinambuzane atholakala kwezentengiselwano akhula kahle ekukhuleni kokunamathela noma kokulengiswa.
Amaseli akhuliswe ngokumiswa angagcinwa emaflaskini okukhulisa angazange alashwe ukuze akhuliswe izicubu, kodwa njengoba ivolumu nendawo engaphezulu yokukhulisa izicubu ikhula, ukushintshana kwegesi okwanele kuyavinjelwa futhi indawo esetshenziswayo idinga ukushukunyiswa. Lokhu kunyakaza kuvame ukufezwa nge-stirrer yamagnetic noma i-flask ye-erlenmeyer ku-incubator eshukumisayo.
| Isiko Lokunamathela
| Isiko Lokumiswa
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| Ifanele izinhlobo eziningi zamaseli, kufaka phakathi ukukhuliswa kwamaseli ayisisekelo | Okufanelekile kumaseli kungaba ukumiswa okukhuliswe kanye namanye amaseli anganamatheli (isb., amaseli e-hematopoietic) |
| Kudinga ukulima okungaphansi kwesikhathi, kodwa kungahlolwa kalula ngokubona ngaphansi kwe-microscope eguquliwe | Kulula ukukhulisa izitshalo ezincane, kodwa kudinga inani lamaseli nsuku zonke kanye nokuhlolwa kokuphila ukuze kubonwe ukukhula; izitshalo zingancishiswa ukuze kukhuthazwe ukukhula. |
| Amaseli ahlukaniswa ngokwe-enzyme (isb. i-trypsin) noma ahlukaniswe ngomshini | Akukho ukuhlukaniswa kwe-enzyme noma kwemishini okudingekayo |
| Ukukhula kunqunyelwe indawo engaphezulu, okungase kunciphise isivuno sokukhiqiza | Ukukhula kunqunyelwe ukuhlushwa kwamaseli endaweni ephakathi, ngakho-ke kungandiswa kalula |
| Imithambo yokukhulisa amaseli edinga ukwelashwa kokukhula kwezicubu | Ingagcinwa emithanjeni yokukhulisa ngaphandle kokwelashwa kobuso bokukhulisa izicubu, kodwa idinga ukushukunyiswa (okungukuthi, ukushukunyiswa noma ukushukunyiswa) ukuze kushintshwe igesi ngokwanele |
| Isetshenziselwa i-cytology, ukuqoqwa kwamaseli okuqhubekayo kanye nezinhlelo eziningi zocwaningo | Isetshenziselwa ukukhiqizwa kwamaprotheni amaningi, ukuqoqwa kwamaseli e-batch kanye nezinhlelo eziningi zocwaningo |
| Thola eyakhoIsithwali se-CO2kanye namapuleti okukhulisa amaseli manje:I-C180 140°C Ukuhlanza Okuphezulu kwe-CO2 IncubatorIpuleti Lokukhuliswa Kweseli | Thola wenaIsishaqisi se-CO2kanye namaflaski e-erlenmeyer manje: |
Isikhathi sokuthunyelwe: Jan-03-2024