Qhov txawv ntawm kev cog qoob loo ntawm cov hlwb adherent thiab kev cog qoob loo ntawm cov hlwb suspension yog dab tsi?

Feem ntau cov hlwb los ntawm cov vertebrates, tshwj tsis yog cov hlwb hematopoietic thiab ob peb lwm cov hlwb, yog cov uas nyob ntawm cov adherent thiab yuav tsum tau cog rau ntawm ib qho substrate uas tau kho tshwj xeeb kom cov hlwb lo thiab kis tau. Txawm li cas los xij, ntau lub hlwb kuj tsim nyog rau kev cog qoob loo. Ib yam li ntawd, feem ntau cov hlwb kab uas muaj nyob hauv khw loj hlob zoo hauv kev cog qoob loo adherent lossis kev cog qoob loo.

Cov qe ntshav uas tau muab tso rau hauv cov thoob uas tsis tau kho kom zoo rau kev cog qoob loo ntawm cov nqaij, tab sis thaum qhov ntim thiab thaj chaw ntawm kev cog qoob loo nce ntxiv, kev sib pauv roj txaus yuav raug cuam tshuam thiab cov khoom siv yuav tsum tau co. Qhov kev co no feem ntau ua tiav los ntawm lub tshuab sib nqus lossis lub raj mis erlenmeyer hauv lub tshuab co.

Kev Cai Lij Choj	Kev Cai Lij Choj Txog Kev Ncua
Haum rau feem ntau hom cell, suav nrog cov kab lis kev cai cell thawj zaug	Haum rau cov hlwb tuaj yeem raug tso rau hauv cov kab mob thiab qee cov hlwb tsis lo rau (piv txwv li, cov hlwb hematopoietic)
Yuav tsum tau ua kom cov tsiaj txhu tsis tu ncua, tab sis tuaj yeem yooj yim saib xyuas hauv qab lub tshuab microscope uas tig rov qab	Yooj yim dua rau kev cog qoob loo me me, tab sis xav tau kev suav cov cell txhua hnub thiab kev ntsuam xyuas kev muaj sia nyob kom pom kev loj hlob; cov kab lis kev cai tuaj yeem raug diluted kom txhawb kev loj hlob
Cov hlwb yog enzymatically (piv txwv li trypsin) lossis mechanically dissociated	Tsis tas yuav tsum muaj enzymatic lossis mechanical dissociation
Kev loj hlob raug txwv los ntawm thaj chaw saum npoo, uas yuav txwv cov txiaj ntsig ntawm kev tsim khoom	Kev loj hlob raug txwv los ntawm qhov kev sib sau ua ke ntawm cov hlwb hauv nruab nrab, yog li tuaj yeem yooj yim scaled up.
Cov hlab ntsha hauv lub cev uas xav tau kev kho mob ntawm cov ntaub so ntswg	Yuav khaws cia tau hauv cov hlab ntsha uas tsis tas kho qhov chaw ntawm cov ntaub so ntswg, tab sis yuav tsum tau kev co (piv txwv li, co lossis do) kom pauv roj tau zoo
Siv rau cytology, kev sau cov cell tas mus li thiab ntau daim ntawv thov kev tshawb fawb	Siv rau kev tsim cov protein ntau, kev sau cov cell ntau thiab ntau daim ntawv thov kev tshawb fawb
Tau koj liLub tshuab ua kom CO2 sovthiab cov phaj kab lis kev cai tam sim no:C180 140 ° C Kub Kub Sterilization CO2 IncubatorPhaj Kab Lis Kev Cai ntawm Cell	Tau kojCO2 incubator shakerthiab cov fwj erlenmeyer tam sim no:   CS315 UV Sterilization Stackable CO2 Incubator Shaker   Kev Kab Mob Cell Erlenmeyer Flask