Kodi kusiyana pakati pa chikhalidwe cha maselo omatira ndi chikhalidwe cha maselo oimitsidwa ndi kotani?

Maselo ambiri ochokera ku zamoyo zokhala ndi msana, kupatula maselo a hematopoietic ndi maselo ena ochepa, amadalira kwambiri kutsata ndipo ayenera kulimidwa pa nthaka yoyenera yomwe yathandizidwa kuti maselo azigwirizana ndikufalikira. Komabe, maselo ambiri ndi oyeneranso kukulitsa kutsamira. Mofananamo, maselo ambiri a tizilombo omwe amapezeka m'masitolo amakula bwino mu kutsamira kapena kutsamira.

Maselo opangidwa ndi suspension-cultured cells amatha kusungidwa m'mabotolo opangidwa ndi suspension omwe sanachiritsidwe kuti apangidwe ndi minofu, koma pamene kuchuluka ndi malo opangidwa ndi suspension akuwonjezeka, kusinthana kwa mpweya kokwanira kumalephereka ndipo malo opangidwa ndi suspension amafunika kusunthidwa. Kusunthika kumeneku nthawi zambiri kumachitika ndi magnetic stirrer kapena erlenmeyer flask mu shaking incubator.

Chikhalidwe Chotsatira	Chikhalidwe Choyimitsidwa
Yoyenera mitundu yambiri ya maselo, kuphatikizapo kukula kwa maselo oyambira	Oyenera maselo akhoza kukulitsidwa ndi kuimitsidwa ndi maselo ena osagwira ntchito (monga maselo a hematopoietic)
Zimafuna kubzala nthawi ndi nthawi, koma zimatha kuwonedwa mosavuta pogwiritsa ntchito maikulosikopu yozungulira	Kulima pang'ono n'kosavuta, koma kumafuna kuchuluka kwa maselo tsiku ndi tsiku ndi kuyesa momwe angakulire; kubzala kungachepe kuti kulimbikitse kukula.
Maselo amapangidwa ndi enzyme (monga trypsin) kapena amalekanitsidwa ndi makina	Palibe kugawanika kwa enzyme kapena makina komwe kumafunika
Kukula kumachepetsedwa ndi malo ozungulira, zomwe zingachepetse zokolola	Kukula kumachepa ndi kuchuluka kwa maselo m'thupi, kotero kumatha kukulitsidwa mosavuta
Mitsempha yokulitsa maselo yomwe imafuna chithandizo cha pamwamba pa minofu	Zitha kusungidwa m'mitsempha ya zomera popanda kuchiza pamwamba pa minofu, koma zimafunika kusunthidwa (monga kugwedezeka kapena kusunthidwa) kuti mpweya usinthe bwino.
Amagwiritsidwa ntchito pa cytology, kusonkhanitsa maselo mosalekeza komanso ntchito zambiri zofufuza	Amagwiritsidwa ntchito popanga mapuloteni ambiri, kusonkhanitsa maselo a batch ndi ntchito zambiri zofufuza
Pezani yanuChosungiramo mpweya cha CO2ndi mbale zokulitsa maselo tsopano:Chotengera cha CO2 Chotenthetsera Kutentha Kwambiri cha C180 140°CMbale Yopangira Chikhalidwe cha Cell	Pezani inuChotsukira choyatsira CO2ndi ma flask a erlenmeyer tsopano:   Chotsukira Chotsukira Chokhazikika cha CO2 cha CS315 UV   Chikhalidwe cha Cell Erlenmeyer Botolo