Chifukwa chiyani CO2 ikufunika mu chikhalidwe cha ma cell?

PH ya njira yodziwika bwino yama cell ndi pakati pa 7.0 ndi 7.4. Popeza carbonate pH buffer system ndi physiological pH buffer system (ndi yofunika pH buffer system m'magazi aumunthu), imagwiritsidwa ntchito kusunga pH yokhazikika m'zikhalidwe zambiri. kuchuluka kwa sodium bicarbonate nthawi zambiri kumafunika kuwonjezeredwa pokonzekera zikhalidwe ndi ufa. Kwa zikhalidwe zambiri zomwe zimagwiritsa ntchito carbonate ngati pH buffer system, kuti pH ikhale yokhazikika, mpweya woipa wa carbon dioxide mu incubator uyenera kusungidwa pakati pa 2-10% kuti ukhalebe wosungunuka wa carbon dioxide mu njira yothetsera chikhalidwe. Nthawi yomweyo zombo zama cell zimayenera kukhala zopumira pang'ono kuti zilole kusinthana kwa gasi.

Kodi kugwiritsa ntchito makina ena a pH buffer kumathetsa kufunikira kwa chofungatira cha CO2? Zapezeka kuti chifukwa cha kuchepa kwa mpweya woipa m'mlengalenga, ngati maselo sanapangidwe mu chofungatira cha carbon dioxide, HCO3- mu chikhalidwe cha chikhalidwe chidzatha, ndipo izi zidzasokoneza kukula kwa maselo. Chifukwa chake ma cell ambiri a nyama amakulitsidwabe mu chofungatira cha CO2.

Pazaka makumi angapo zapitazi, magawo a biology ya cell, biology ya mamolekyulu, pharmacology, ndi zina zambiri apita patsogolo modabwitsa pa kafukufuku, ndipo panthawi imodzimodziyo, kugwiritsa ntchito ukadaulo m'magawo awa kwakhala kukuyenda. Ngakhale zida za labotale ya sayansi ya moyo zasintha kwambiri, chofungatira cha CO2 akadali gawo lofunikira la labotale, ndipo amagwiritsidwa ntchito ndi cholinga chosunga ndi kulimbikitsa kukula kwa maselo ndi minofu. Komabe, ndi kupita patsogolo kwaukadaulo, ntchito yawo ndi magwiridwe antchito zakhala zolondola, zodalirika komanso zosavuta. Masiku ano, ma incubators a CO2 akhala chimodzi mwa zida zomwe zimagwiritsidwa ntchito nthawi zonse m'ma laboratories ndipo zakhala zikugwiritsidwa ntchito kwambiri pofufuza ndi kupanga pazamankhwala, chitetezo chamthupi, genetics, microbiology, sayansi yaulimi, ndi pharmacology.

Chofungatira cha CO2 chimapanga malo oti ma cell/minofu ikule bwino poyendetsa chilengedwe. Zotsatira za kuwongolera mkhalidwewo zimapanga mkhalidwe wokhazikika: mwachitsanzo acidity/alkalinity yosalekeza (pH: 7.2-7.4), kutentha kokhazikika (37°C), chinyezi chambiri (95%), ndi mulingo wokhazikika wa CO2 (5%), ndichifukwa chake ofufuza m’magawo omwe ali pamwambawa ali okondwa kwambiri ndi mwayi wogwiritsa ntchito chofungatira cha CO2.

Kuphatikiza apo, ndi kuwonjezera kwa CO2 kuwongolera ndende komanso kugwiritsa ntchito kachipangizo kakang'ono kuti kawongolere kutentha kwa chofungatira, kupambana kwabwino komanso luso la kulima ma cell achilengedwe ndi minyewa, etc., zasinthidwa. Mwachidule, chofungatira cha CO2 ndi mtundu watsopano wa chofungatira chomwe sichingasinthidwe ndi chofungatira wamba chamagetsi chamagetsi m'ma laboratories achilengedwe.