Kutheni usebenzisa i-CO2 incubator ukuze kukhule iiseli?

I-pH yesisombululo esiqhelekileyo senkcubeko yeseli iphakathi kwe-7.0 kunye ne-7.4. Ekubeni inkqubo ye-carbonate pH buffer iyinkqubo ye-physiological pH buffer (yinkqubo ebalulekileyo ye-pH buffer egazini lomntu), isetyenziselwa ukugcina i-pH ezinzileyo kwiinkcubeko ezininzi. Isixa esithile se-sodium bicarbonate sidla ngokufuna ukongezwa xa kulungiswa iinkcubeko ezineempuphu. Kwiinkcubeko ezininzi ezisebenzisa i-carbonate njengenkqubo ye-pH buffer, ukuze kugcinwe i-pH ezinzileyo, i-carbon dioxide kwi-incubator kufuneka igcinwe phakathi kwe-2-10% ukuze kugcinwe uxinzelelo lwe-carbon dioxide enyibilikisiweyo kwisisombululo senkcubeko. Kwangaxeshanye, imikhumbi yenkcubeko yeseli kufuneka ikwazi ukuphefumla kancinci ukuze kuvunyelwe ukutshintshiselana ngegesi.

Ngaba ukusetyenziswa kwezinye iinkqubo ze-pH buffer kuyayiphelisa imfuneko ye-Isifukamisi se-CO2Kuye kwafunyaniswa ukuba ngenxa yoxinzelelo oluphantsi lwekhabhoni diokside emoyeni, ukuba iiseli azikhuliswanga kwi-incubator yekhabhoni diokside, i-HCO3- kwi-culture medium iya kuncipha, kwaye oku kuya kuphazamisa ukukhula okuqhelekileyo kweeseli. Ngoko ke uninzi lweeseli zezilwanyana zisakhuliswa kwi-incubator ye-CO2.

Kwiminyaka embalwa edlulileyo, amacandelo ebhayoloji yeseli, ibhayoloji yemolekyuli, i-pharmacology, njl.njl. enze inkqubela emangalisayo kuphando, kwaye kwangaxeshanye, ukusetyenziswa kwetekhnoloji kwezi ndawo kuye kwafuneka kuhambisane nesantya. Nangona izixhobo zelabhoratri zesayensi yobomi eziqhelekileyo zitshintshile kakhulu, i-CO2 incubator iseyinxalenye ebalulekileyo yelabhoratri, kwaye isetyenziselwa injongo yokugcina nokukhuthaza ukukhula okungcono kweeseli kunye nezicubu. Nangona kunjalo, ngokuqhubela phambili kwetekhnoloji, umsebenzi kunye nokusebenza kwazo kuye kwaba ngokuchanekileyo, kuthembekile kwaye kulula. Kule mihla, i-CO2 incubators ziye zaba zezinye zezixhobo eziqhelekileyo ezisetyenziswa kakhulu kwiilabhoratri kwaye zisetyenziswa kakhulu kuphando nakwimveliso kwezonyango, kwi-immunology, kwi-genetics, kwi-microbiology, kwisayensi yezolimo, nakwi-pharmacology.

I-CO2 incubator idala imeko-bume yokukhula ngcono kweeseli/izicubu ngokulawula iimeko zendalo ezingqongileyo. Isiphumo solawulo lwemeko sidala imeko ezinzileyo: umz. i-acidity/alkalinity engaguqukiyo (pH: 7.2-7.4), ubushushu obuzinzileyo (37°C), ukufuma okuphezulu (95%), kunye nenqanaba le-CO2 elizinzileyo (5%), yiyo loo nto abaphandi kwiinkalo ezingasentla benomdla kakhulu malunga nokulula kokusebenzisa i-CO2 incubator.

Ukongeza, ngokongezwa kolawulo loxinzelelo lwe-CO2 kunye nokusetyenziswa kwe-microcontroller ukulawula ubushushu obuchanekileyo be-incubator, izinga lempumelelo kunye nokusebenza kakuhle kokulima iiseli zebhayoloji kunye nezicubu, njl.njl., kuye kwaphuculwa. Ngamafutshane, i-CO2 incubator luhlobo olutsha lwe-incubator olungenakutshintshwa yi-incubator eqhelekileyo ye-thermostat kwiilabhoratri zebhayoloji.