Sei uchishandisa CO2 incubator pakukura kwemasero?

pH yemushonga wekukura kwemasero uri pakati pe7.0 ne7.4. Sezvo carbonate pH buffer system iri physiological pH buffer system (inokosha pH buffer system muropa remunhu), inoshandiswa kuchengetedza pH yakagadzikana mumarudzi mazhinji ezvirimwa. huwandu hwakati hwesodium bicarbonate hunowanzo fanirwa kuwedzerwa pakugadzira cultures dzine hupfu. Kune zvizvarwa zvakawanda zvinoshandisa carbonate se pH buffer system, kuti kuchengetedza pH yakagadzikana, carbon dioxide iri mu incubator inofanira kuchengetedzwa pakati pe2-10% kuchengetedza huwandu hwe carbon dioxide yakanyungudutswa mumushonga wekukura. Panguva imwe chete midziyo yekukura kwemasero inofanirwa kunge yakapfava zvishoma kuti ibvumire kuchinjana kwegasi.

Ko kushandiswa kwemamwe ma pH buffer systems kunobvisa here kudiwa kweChinotonhorera cheCO2Zvakaonekwa kuti nekuda kwekuwanda kwecarbon dioxide mumhepo, kana masero asina kurimwa mu carbon dioxide incubator, HCO3- iri mu culture medium ichapera, uye izvi zvichakanganisa kukura kwemasero. Saka masero mazhinji emhuka achiri kurimwa mu CO2 incubator.

Mumakumi emakore apfuura, minda yebiology yemasero, molecular biology, pharmacology, nezvimwewo yakaita kufambira mberi kukuru mukutsvagisa, uye panguva imwe chete, kushandiswa kwetekinoroji muminda iyi kwave kuchifanira kuenderera mberi. Kunyangwe michina yerabhoritari yesainzi yehupenyu yakachinja zvakanyanya, CO2 incubator ichiri chikamu chakakosha cherabhoritari, uye inoshandiswa nechinangwa chekuchengetedza nekusimudzira kukura kuri nani kwemasero nenyama. Zvisinei, nekufambira mberi kwetekinoroji, mashandiro avo uye mashandiro avo ave akanyatsojeka, akavimbika uye ari nyore. Mazuva ano, CO2 incubators yave imwe yemidziyo inowanzo shandiswa murabhoritari uye yave ichishandiswa zvakanyanya mukutsvagisa nekugadzira mishonga, immunology, genetics, microbiology, agricultural science, uye pharmacology.

Chinotakurwa neCO2 chinogadzira nzvimbo yekukura kwemasero/nyama zviri nani nekudzora mamiriro ezvakatipoteredza. Mhedzisiro yekudzora mamiriro ezvinhu inoita kuti pave nemamiriro akagadzikana: semuenzaniso acidity/alkalinity inogara iripo (pH: 7.2-7.4), tembiricha yakagadzikana (37°C), hunyoro hwakanyanya (95%), uye CO2 level yakagadzikana (5%), ndosaka vaongorori vari pamusoro apa vachifarira zvikuru kushandisa CO2 incubator.

Pamusoro pezvo, nekuwedzera kudzora huwandu hweCO2 uye kushandiswa kwe microcontroller yekudzora tembiricha chaiyo ye incubator, mwero wekubudirira uye kushanda zvakanaka kwekurima masero ebhayoloji nematishu, nezvimwewo, zvavandudzwa. Muchidimbu, CO2 incubator rudzi rutsva rwe incubator rusingatsiviwe ne incubator yemagetsi ye thermostat mumarabhoritari ebhayoloji.