Kungani kufanele usebenzise i-CO2 incubator ukuze kukhuliswe amaseli?

I-pH yesisombululo esivamile sokukhuliswa kwamaseli iphakathi kuka-7.0 no-7.4. Njengoba uhlelo lokukhuliswa kwe-pH ye-carbonate luyisistimu yokukhuliswa kwe-pH yomzimba (luyisistimu ebalulekile yokukhuliswa kwe-pH egazini lomuntu), lusetshenziselwa ukugcina i-pH ezinzile emasikweni amaningi. Inani elithile le-sodium bicarbonate livame ukudinga ukungezwa lapho kulungiswa ama-cultures anempuphu. Kwama-cultures amaningi asebenzisa i-carbonate njengesistimu yokukhuliswa kwe-pH, ukuze kugcinwe i-pH ezinzile, i-carbon dioxide ese-incubator idinga ukugcinwa phakathi kuka-2-10% ukuze kugcinwe ukuhlushwa kwe-carbon dioxide encibilikisiwe esixazululweni sokukhuliswa. Ngesikhathi esifanayo imikhumbi yokukhuliswa kwamaseli idinga ukuphefumula kancane ukuze ivumele ukushintshaniswa kwegesi.

Ingabe ukusetshenziswa kwezinye izinhlelo ze-pH buffer kuqeda isidingo se-Isithwali se-CO2Kutholakale ukuthi ngenxa yokugcwala okuphansi kwe-carbon dioxide emoyeni, uma amaseli engakhuliswanga ku-incubator ye-carbon dioxide, i-HCO3- endaweni yokukhulisa izophela, futhi lokhu kuzophazamisa ukukhula okuvamile kwamaseli. Ngakho-ke iningi lamaseli ezilwane lisakhuliswa ku-incubator ye-CO2.

Emashumini ambalwa eminyaka edlule, imikhakha yebhayoloji yamaseli, i-molecular biology, i-pharmacology, njll. yenze intuthuko emangalisayo ocwaningweni, futhi ngesikhathi esifanayo, ukusetshenziswa kobuchwepheshe kulezi zinsimu kuye kwadingeka kuhambisane nesivinini. Nakuba imishini ejwayelekile yelabhorethri yesayensi yokuphila ishintshile kakhulu, i-CO2 incubator iseyingxenye ebalulekile yelabhorethri, futhi isetshenziselwa ukugcina nokukhuthaza ukukhula okungcono kwamaseli nezicubu. Kodwa-ke, ngentuthuko kwezobuchwepheshe, umsebenzi nokusebenza kwazo kuye kwaba okunembile, okuthembekile nokulula. Namuhla, i-CO2 incubators isibe ngesinye sezinsimbi ezivamile ezisetshenziswa kakhulu emalabhorethri futhi isetshenziswe kabanzi ocwaningweni nasekukhiqizweni kwezokwelapha, i-immunology, izakhi zofuzo, i-microbiology, isayensi yezolimo, kanye ne-pharmacology.

I-CO2 incubator idala indawo yokukhula okungcono kwamaseli/izicubu ngokulawula izimo zemvelo ezizungezile. Umphumela wokulawulwa kwesimo udala isimo esizinzile: isib. i-acidity/alkalinity engaguquki (pH: 7.2-7.4), izinga lokushisa elizinzile (37°C), umswakama ophezulu (95%), kanye nezinga le-CO2 elizinzile (5%), yingakho abacwaningi emikhakheni engenhla benomdlandla kakhulu ngokulula kokusebenzisa i-CO2 incubator.

Ngaphezu kwalokho, ngokufakwa kokulawulwa kokuhlushwa kwe-CO2 kanye nokusetshenziswa kwe-microcontroller ukulawula izinga lokushisa elinembile le-incubator, izinga lempumelelo kanye nokusebenza kahle kokutshalwa kwamaseli nezicubu zezinto eziphilayo, njll., kuye kwathuthukiswa. Ngamafuphi, i-CO2 incubator uhlobo olusha lwe-incubator olungenakufakwa esikhundleni se-incubator kagesi evamile ye-thermostat emalabhorethri ezinto eziphilayo.