umuco wo guhagarika umuco ni uwuhe?
Ingirabuzimafatizo nyinshi ziva mu nyababyeyi, usibye selile hematopoietic selile hamwe nizindi selile nkeya, zishingiye ku bayoboke kandi zigomba guterwa hejuru yubutaka buboneye bwavuwe byumwihariko kugirango ingirabuzimafatizo zifatanye kandi zikwirakwira. Nyamara, selile nyinshi nazo zikwiranye numuco wo guhagarika. Mu buryo nk'ubwo, ingirabuzimafatizo nyinshi ziboneka mu bucuruzi zikura neza haba mu muco wo kubahiriza cyangwa guhagarika.
Ingirabuzimafatizo zishingiye ku guhagarika umutima zishobora kubikwa mu bikoresho by’umuco bitavuwe ku muco w’inyama, ariko uko ingano n’ubuso bw’umuco byiyongera, guhanahana gaze bihagije birabangamiwe kandi bigomba gukangurwa. Iyi myigaragambyo isanzwe igerwaho na magnetiki stirrer cyangwa flask ya erlenmeyer muguhungabanya incubator.
| Umuco w'abayoboke  | Umuco wo Guhagarika  |
| Birakwiriye kubwoko bwinshi, harimo umuco wibanze | Bikwiranye na selile birashobora guhagarikwa byimico hamwe nizindi selile zidakurikiza (urugero, hematopoietic selile) |
| Irasaba subculture yigihe, ariko irashobora kugenzurwa byoroshye munsi ya microscope idahindutse | Byoroshye kurwego rwumuco, ariko bisaba kubara buri munsi ingirabuzimafatizo hamwe nubushobozi bwo kwitegereza gukura; imico irashobora kuvangwa kugirango itere imbere |
| Ingirabuzimafatizo zidafite imbaraga (urugero trypsin) cyangwa zitandukanye | Nta enzymatique cyangwa imashini itandukanye isabwa |
| Gukura kugarukira kubuso, bushobora kugabanya umusaruro | Gukura kugarukira hamwe no kwibanda kwingirabuzimafatizo hagati, bityo birashobora kwaguka byoroshye |
| Imiyoboro yumuco yingirabuzimafatizo isaba kuvura umuco | Irashobora kubungabungwa mumitsi yumuco idafite ubuvuzi bwimiterere yumubiri, ariko bisaba guhagarika umutima (ni ukuvuga kunyeganyega cyangwa gukurura) kugirango habeho guhana gazi ihagije |
| Byakoreshejwe kuri cytologiya, gukusanya selile hamwe nubushakashatsi bwinshi | Ikoreshwa mubikorwa byinshi bya poroteyine, gukusanya ingirabuzimafatizo hamwe nubushakashatsi bwinshi |
| Shaka CO2 incubator hamwe nicyapa cyumuco utugari ubu:C180 140 ° C Ubushyuhe Bwinshi bwo Kurwanya CO2 IncubatorIcyapa cy'umuco w'akagari | Shaka CO2 incubator shaker na flasks ya erlenmeyer ubungubu: |
Igihe cyo kohereza: Kanama-28-2023