yintoni ukunqunyanyiswa kwenkcubeko yeseli vs ukubambelela?
Uninzi lweeseli ezivela kwi-vertebrates, ngaphandle kweeseli ze-hematopoietic kunye nezinye iiseli ezimbalwa, zixhomekeke-ezixhomekeke kwaye kufuneka zikhuliswe kwi-substrate efanelekileyo eye yaphathwa ngokukodwa ukuvumela ukunamathela kweeseli kunye nokusabalalisa. Nangona kunjalo, iiseli ezininzi zikwafanelekile kwinkcubeko yokunqunyanyiswa. Ngokufanayo, uninzi lweeseli zezinambuzane ezithengiswayo zikhula kakuhle ekubambeni okanye ekunqunyanyisweni.
Iiseli zenkcubeko ezimisiweyo zingagcinwa kwiiflasks zenkcubeko ezingakhange ziphathwe kwinkcubeko ye-tissue, kodwa njengoko umthamo kunye nommandla wenkcubeko ukhula, ukutshintshiselana kwegesi okwaneleyo kuthintelwe kwaye okuphakathi kufuneka kuphazamiseke. Oku kuphazamiseka kudla ngokufezekiswa nge-magnetic stirrer okanye i-erlenmeyer flask kwi-shaking incubator.
| Inkcubeko ebambeleleyo  | Ukunqunyanyiswa kweNkcubeko  |
| Ilungele uninzi lweentlobo zeeseli, kubandakanya inkcubeko yeseli esisiseko | Ifanele iiseli inokunqunyanyiswa kunye nezinye iiseli ezingabambeleliyo (umzekelo, iiseli ze-hematopoietic) |
| Ifuna i-periodic subculture, kodwa inokuhlolwa ngokulula ngokubonakalayo phantsi kwe-microscope eguqulweyo | Kulula kwi-subculture, kodwa ifuna ukubalwa kweeseli zemihla ngemihla kunye novavanyo olusebenzayo ukujonga ukukhula; Iinkcubeko zinokungxengwa ukuze zikhuthaze ukukhula |
| Iiseli ze-enzymatic (umzekelo, i-trypsin) okanye zahlulwa ngoomatshini | Akukho ukwahlukana kwe-enzymatic okanye komatshini okufunekayo |
| Ukukhula kuthintelwe ngummandla ongaphezulu, nto leyo enokunciphisa imveliso yemveliso | Ukukhula kuthintelwe kukuxinana kweeseli phakathi, ngoko kunokunyuswa ngokulula |
| Iinqanawa zenkcubeko yeeseli ezifuna unyango lomphezulu lwethishu | Inokugcinwa kwiinqanawa zenkcubeko ngaphandle konyango lwe-tissue yenkcubeko yomphezulu, kodwa ifuna ukuphazamiseka (oko kukuthi, ukungcangcazela okanye ukushukumisa) ukutshintshiselana kwegesi okwaneleyo. |
| Isetyenziselwa i-cytology, ukuqokelela kweeseli eziqhubekayo kunye nezicelo ezininzi zophando | Isetyenziselwa ukuveliswa kweeprotheyini ezininzi, ukuqokelelwa kweeseli zebhetshi kunye nezicelo ezininzi zophando |
| Fumana i-CO2 incubator kunye neepleyiti zenkcubeko yeseli ngoku:I-C180 140 °C Ukushisa okuphezulu kwe-CO2 IncubatorCell Culture Plate | Zifumanele i-CO2 incubator shaker kunye neeflaski ze-erlenmeyer ngoku: |
Ixesha lokuposa: Aug-28-2023