Ungakhetha kanjani i-amplitude ye-Shaker efanele?
Iyini i-amplitude ye-shaker?
I-amplitude ye-shaker ububanzi bephalethi ekuhambeni okuyindilinga, ngezinye izikhathi okubizwa ngokuthi "i-oscillation diameter" noma "i-track diameter": Ø. I-Radobio inikeza ama-shaker ajwayelekile anama-amplitudes angu-3mm, 25mm, 26mm no-50mm,. Amashaker enziwe ngokwezifiso anamanye amasayizi we-amplitude ayatholakala.
Liyini Isilinganiso Sokudlulisa I-Oxygen (OTR)?
I-Oxygen Transfer Rate (OTR) ukusebenza kahle komoya-mpilo udluliswa usuka emkhathini uye oketshezini. Ukuphakama kwevelu ye-OTR kusho ukuphakama kokudluliswa kwe-oxygen kahle.
Umthelela we-amplitude kanye nesivinini sokuzungeza
Zombili lezi zici zithinta ukuxutshwa kwento ephakathi eflaskini yesiko. Ukuxuba okungcono kakhulu, kuba ngcono izinga lokudlulisa umoya-mpilo (OTR). Ukulandela lezi ziqondiso, i-amplitude efanelekile kakhulu nesivinini sokujikeleza singakhethwa.
Ngokuvamile, ukukhetha i-amplitude engu-25mm noma 26mm kungasetshenziswa njenge-amplitude yendawo yonke yazo zonke izinhlelo zokusebenza zesiko.
Amagciwane, imvubelo kanye namasiko fungal:
Ukudluliswa komoyampilo kumaflask okunyakazisa akusebenza kahle kakhulu kunakuma-bioreactors. Ukudluliswa komoyampilo kungase kube isici esikhawulelayo kumasiko e-shake flask ezimweni eziningi. I-amplitude ihlobene nobukhulu be-conical flasks: ama-flasks amakhulu asebenzisa ama-amplitudes amakhulu.
Isincomo: i-amplitude engu-25mm yamaflasks ayi-conical kusuka ku-25ml kuya ku-2000ml.
50 mm amplitude for flasks conical kusuka 2000 ml kuya 5000 ml.
I-Cell Culture:
* Isiko lamaseli ezilwane ezincelisayo anesidingo somoya-mpilo esiphansi uma kuqhathaniswa.
* Kumaflask e-shaker angu-250mL, ukulethwa komoyampilo owanele kungahlinzekwa ngebanga elibanzi kakhulu lama-amplitude nesivinini (20-50mm amplitude; 100-300rpm).
* Kumaflasks amakhulu (amaflaski eFernbach) kunconywa i-amplitude engu-50mm.
* Uma kusetshenziswa izikhwama zamasiko alahlwayo, kunconywa i-50mm amplitude.
Amapuleti ama-Microtiter namapuleti ajulile:
Kumapuleti ama-microtiter namapuleti ajulile kunezindlela ezimbili ezihlukene zokuthola ukudluliswa komoya-mpilo okuphezulu!
* 50 mm amplitude ngesivinini esingekho ngaphansi kuka-250 rpm.
* Sebenzisa i-3mm amplitude ku-800-1000rpm.
Ezimweni eziningi, ngisho noma i-amplitude enengqondo ikhethiwe, ingase ingakhulisi ivolumu ye-bioculture, ngoba ukwanda kwevolumu kungathonywa yizici eziningana. Isibonelo, uma isici esisodwa noma ezimbili kweziyishumi zingalungile, khona-ke ukwanda kwevolumu yesiko kuzokhawulwa kungakhathaliseki ukuthi ezinye izici zinhle kangakanani, noma kungase kuthiwe ukukhetha okufanele kwe-amplitude kuzoholela ekwenyukeni okuphawulekayo kwe-incubator uma okuwukuphela kwesici esinqunyelwe kumthamo wesiko ukulethwa komoyampilo. Isibonelo, uma umthombo wekhabhoni uyisici esikhawulelayo, kungakhathaliseki ukuthi kuhle kangakanani ukudluliswa komoya-mpilo, ivolumu yesiko elifiswayo ngeke lizuzwe.
I-amplitude kanye nesivinini sokuzungeza
Kokubili i-amplitude nesivinini sokujikeleza kungaba nomthelela ekudlulisweni komoyampilo. Uma amasiko amaseli etshalwa ngesivinini esiphansi kakhulu sokujikeleza (isb., 100 rpm), umehluko we-amplitude unomthelela omncane noma ongabonakali ekudluliselweni komoyampilo. Ukuze kuzuzwe ukudluliswa kwe-oxygen ephakeme kakhulu, isinyathelo sokuqala ukwandisa isivinini sokujikeleza ngangokunokwenzeka, futhi ithreyi izoba nokulinganisela kahle ngesivinini. Akuwona wonke amaseli angakhula kahle ngokuzungeza kwesivinini esikhulu, futhi amanye amaseli azwela amandla okugunda angase afe ngenxa yesivinini esikhulu sokujikeleza.
Amanye amathonya
Ezinye izici zingaba nomthelela ekudlulisweni komoyampilo:.
* Ivolumu yokugcwalisa, ama-flasks ayi-conical kufanele agcwaliswe angabi ngaphezu kwengxenye eyodwa kwezintathu yevolumu ephelele. Uma ukudluliswa kwe-oxygen okuphezulu kuzofezwa, gcwalisa ungabi ngaphezu kuka-10%. Ungalokothi ugcwalise ku-50%.
* Ama-Spoilers: Ama-spoilers ayasebenza ekuthuthukiseni ukudluliswa komoya-mpilo kuzo zonke izinhlobo zamasiko. Abanye abakhiqizi batusa ukusetshenziswa kwamaflask "I-Ultra High Yield". Ama-spoiler kulawa maflask akhuphula ukungqubuzana koketshezi futhi isinyakazi singase singafinyeleli isivinini esibekiwe.
Ukuhlobana phakathi kwe-amplitude nesivinini
Amandla we-centrifugal ku-shaker angabalwa kusetshenziswa i-equation elandelayo
FC = rpm2× ubukhulu
Kukhona ubudlelwano bomugqa phakathi kwamandla we-centrifugal kanye ne-amplitude: uma usebenzisa i-amplitude engu-25 mm ukuya ku-50 mm amplitude (ngesivinini esifanayo), amandla e-centrifugal akhuphuka nge-factor of 2.
Ubudlelwano obuyisikwele bukhona phakathi kwamandla we-centrifugal kanye nesivinini sokujikeleza.
Uma ijubane likhushulwa nge-2 (amplitude efanayo), amandla e-centrifugal akhuphuka nge-4. Uma ijubane lenyuka nge-3 factor, amandla e-centrifugal akhuphuka nge-9 factor!
Uma usebenzisa i-amplitude engu-25 mm, fukamela ngesivinini esinikeziwe. Uma ufisa ukuzuza amandla afanayo e-centrifugal nge-amplitude engu-50 mm, isivinini sokujikeleza kufanele sibalwe njengempande yesikwele engu-1/2, ngakho-ke kufanele usebenzise u-70% wejubane lokujikeleza ukuze ufinyelele izimo ezifanayo zokufukamela.
Sicela uqaphele ukuthi lokhu okungenhla kuyindlela yasetiyetha kuphela yokubala amandla e-centrifugal. Kukhona ezinye izici ezinomthelela ezinhlelweni zangempela. Le ndlela yokubala inikeza amanani acishe abe yizinjongo zokusebenza.
Isikhathi sokuthumela: Sep-10-2023