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Ingakanani i-medium okufanele ifakwe ebhodleleni ku-incubator enyakazayo?
Amaflaski e-Erlenmeyer e-RADOBIO ayisithombo aklanyelwe ngqo ukukhulisa amaseli okumiswa okuhlanganisa amaseli e-HEK 293 kanye ne-CHO ku-incubator enyakazayo. Lawa maflaski anesisekelo esisicaba kanye nesivalo esinomoya, okwenza afaneleke ekuthuthukisweni kwenqubo encane, ukukhulisa, kanye ...Funda kabanzi -
Iyini i-CO₂ Incubator? Umhlahlandlela Osheshayo
Nakuba isetshenziswa kabanzi kwezesayensi yokuphila, imisebenzi ye-CO₂ incubator ayikajwayelekile kwabaningi. Lo mhlahlandlela uchaza injongo yayo, ukusebenza kwayo, kanye nezinhlelo zayo—kungakhathaliseki ukuthi use-lab yebhayoloji yeseli, esikhungweni semithi, noma esikhungweni socwaningo lwezokwelapha. Iyini i-CO₂ Incubator? I-CO₂ incubator iyi-en...Funda kabanzi -
Kusebenza kangakanani ukubulala amagciwane okushisa okuphezulu kwe-CO₂ incubator?
Ukungcola kokukhula kwamaseli kuvame ukuba yinkinga evame ukuhlangatshezwana nayo ezindaweni zokucwaninga ngokuthuthukiswa kwamaseli, ngezinye izikhathi kube nemiphumela emibi kakhulu. Ukungcola kokukhula kwamaseli kungahlukaniswa ngezigaba ezimbili eziyinhloko, ukungcola kwamakhemikhali njengokungcola okusezindaweni, i-serum kanye namanzi, ama-endotoxin,...Funda kabanzi -
Kungani i-CO2 incubator yami inomswakama?
Uma sisebenzisa i-CO2 incubator ukukhulisa amaseli, ngenxa yomehluko enanini loketshezi olungeziwe kanye nomjikelezo wokukhuliswa, sinezidingo ezahlukene zomswakama ohlobene ku-incubator. Ukuze kuhlolwe kusetshenziswa amapuleti okukhuliswa kwamaseli angu-96 anomjikelezo omude wokukhuliswa, ngenxa yobukhulu obuncane...Funda kabanzi -
Ungayikhetha kanjani i-amplitude efanele ye-incubator enyakazayo?
Iyini i-amplitude ye-shaker? I-amplitude ye-incubator shaker ububanzi be-pallet ejikelezayo, ngezinye izikhathi ebizwa ngokuthi “ububanzi bokunyakaza” noma “ububanzi bomkhondo”: Ø. I-Radobio inikeza ama-shaker ajwayelekile ane-amplitude engu-3mm, 25mm, 26mm kanye no-50mm,...Funda kabanzi -
Uyini umehluko phakathi kokukhuliswa kwamaseli anamathelayo kanye nokukhuliswa kwamaseli amiswe?
Iningi lamaseli avela ezilwaneni ezinomgogodla, ngaphandle kwamaseli e-hematopoietic kanye namanye amaseli ambalwa, ancike ekunamatheleni futhi kumele akhulele endaweni efanelekile ephathwe ngokuqondile ukuze kuvunyelwe ukunamathela nokusabalala kwamaseli. Kodwa-ke, amaseli amaningi nawo afanelekela ukukhuliswa kokumiswa....Funda kabanzi -
Uyini umehluko phakathi kwenzwa ye-TC kanye nenzwa ye-IR ku-CO₂ incubator?
Uma kukhuliswa amaseli, ukuqinisekisa ukukhula okufanele, izinga lokushisa, umswakama, kanye namazinga e-CO2 kudingeka kulawulwe. Amazinga e-CO2 abalulekile ngoba asiza ukulawula i-pH yendawo yokukhulisa. Uma kune-CO2 eningi kakhulu, izoba ne-asidi kakhulu. Uma kungekho...Funda kabanzi -
Kungani kufanele usebenzise i-CO2 incubator ukuze kukhuliswe amaseli?
I-pH yesisombululo esijwayelekile sokukhuliswa kwamaseli iphakathi kuka-7.0 no-7.4. Njengoba uhlelo lwe-carbonate pH buffer luyisistimu ye-physiological pH buffer (luyisistimu ebalulekile ye-pH buffer egazini lomuntu), lusetshenziselwa ukugcina i-pH ezinzile ezinhlotsheni eziningi zamasiko. Inani elithile le-sodium bicarbonate livame ukudinga ...Funda kabanzi -
Umphumela wokwehluka kwezinga lokushisa ekukhuleni kwamaseli ku-CO2 incubator
Izinga lokushisa liyipharamitha ebalulekile ekukhuleni kwamaseli ngoba lithinta ukuphindaphindeka kwemiphumela. Izinguquko zokushisa ezingaphezu noma ngaphansi kwama-37°C zinomthelela obaluleke kakhulu ku-kinetics yokukhula kwamaseli amaseli ezilwane ezincelisayo, okufana nokwamangqamuzana ebhaktheriya. Izinguquko ekubonakalisweni kwezakhi zofuzo kanye ...Funda kabanzi -
Ukusetshenziswa kwe-shaking incubator ekukhuleni kwamaseli ebhayoloji
Isiko lezinto eziphilayo lihlukaniswe nge-static culture kanye ne-shaking culture. I-Shaking culture, eyaziwa nangokuthi i-suspension culture, iyindlela yokukhulisa lapho amaseli amancane efakwa khona emgodini oketshezi bese efakwa ku-shaker noma ku-oscillator ukuze kuqhume njalo. Isetshenziswa kabanzi ekuhlolweni kokucindezeleka...Funda kabanzi
