Kungani i-CO2 idingeka kusiko lamaseli?

I-pH yesisombululo esivamile se-cell culture iphakathi kuka-7.0 no-7.4. Njengoba i-carbonate pH buffer system iwuhlelo lwe-pH yebhafa ye-physiological (iwuhlelo olubalulekile lwe-pH buffer egazini lomuntu), isetshenziselwa ukugcina i-pH ezinzile kumasiko amaningi. inani elithile le-sodium bicarbonate ngokuvamile lidinga ukungezwa lapho kulungiswa amasiko ngezimpushana. Emasikweni amaningi asebenzisa i-carbonate njengohlelo lwe-pH buffer, ukuze kugcinwe i-pH ezinzile, i-carbon dioxide ku-incubator idinga ukugcinwa phakathi kuka-2-10% ukuze kugcinwe ukugcwala kwe-carbon dioxide encibilikisiwe kusixazululo sesiko. Ngesikhathi esifanayo imikhumbi ye-cellculture kumele iphefumule kancane ukuze ivumele ukushintshaniswa kwegesi.

Ingabe ukusetshenziswa kwezinye izinhlelo ze-pH buffer kuyasiqeda isidingo se-CO2 incubator? Kutholwe ukuthi ngenxa yokugcwala okuphansi kwe-carbon dioxide emoyeni, uma amangqamuzana engakhuliswanga ku-incubator ye-carbon dioxide, i-HCO3- endaweni yesiko izophela, futhi lokhu kuzophazamisa ukukhula okuvamile kwamaseli. Ngakho-ke amaseli ezilwane amaningi asakhuliswa ku-CO2 incubator.

Emashumini ambalwa eminyaka adlule, imikhakha ye-cell biology, i-molecular biology, i-pharmacology, njll. yenze igxathu elimangalisayo ocwaningweni, futhi ngesikhathi esifanayo, ukusetshenziswa kobuchwepheshe kule mikhakha kuye kwadingeka kuhambisane nesivinini. Nakuba imishini yaselabhorethri yesayensi yempilo evamile isishintshe kakhulu, i-CO2 incubator iseyingxenye ebalulekile yaselabhorethri, futhi isetshenziselwa ukunakekela nokukhuthaza ukukhula okungcono kwamangqamuzana nezicubu. Kodwa-ke, ngokuthuthuka kwezobuchwepheshe, umsebenzi wabo nokusebenza kwabo sekunembe kakhulu, kuthembekile futhi kulula. Namuhla, ama-incubator e-CO2 abe ngelinye lamathuluzi avamile avame ukusetshenziswa ezindaweni zokucwaninga futhi asetshenziswe kabanzi ocwaningweni nasekukhiqizeni kwezokwelapha, i-immunology, izakhi zofuzo, i-microbiology, isayensi yezolimo, kanye nesayensi yezokwelapha.

I-CO2 incubator idala indawo yokukhula okungcono kwamaseli/izicubu ngokulawula izimo zemvelo ezizungezile. Umphumela wokulawulwa kwesimo udala isimo esizinzile: isb. i-asidi/i-alkalinity engaguquki (pH: 7.2-7.4), izinga lokushisa elizinzile (37°C), umswakama ophezulu ohlobene (95%), nezinga le-CO2 elizinzile (5%), yingakho abacwaningi emikhakheni engenhla bekuthakasela kakhulu ukuba lula kokusebenzisa i-incubator ye-CO2.

Ukwengeza, ngokungezwa kokulawulwa kokuhlushwa kwe-CO2 kanye nokusetshenziswa kwe-microcontroller yokulawula ukushisa okunembile kwe-incubator, izinga lokuphumelela kanye nokusebenza kahle kokutshalwa kwamangqamuzana e-biological kanye nezicubu, njll, kuye kwathuthukiswa. Ngamafuphi, i-CO2 incubator iwuhlobo olusha lwe-incubator olungakwazi ukushintshwa yi-incubator evamile kagesi ye-thermostat kumalabhorethri ezinto eziphilayo.