03.Lub Yim Hli 2023 | Lub rooj sib tham txog kev txhim kho bioprocess biopharmaceutical

Xyoo 2023 lub rooj sib tham txog kev txhim kho bioprocess biopharmaceutical,radobio koom nrog ua tus neeg muab khoom siv biopharmaceutical cell culture.

Ib txwm muaj, kev tshawb fawb txog tsiaj txhu hauv chav kuaj mob yog ib qho kev ua haujlwm me me; cov hlab ntsha ntawm cov nqaij mos tsis tshua loj dua li tus neeg sim tes, qhov ntim raug ntsuas hauv "milliliters," thiab kev ntxuav cov protein raug suav hais tias yog kev vam meej yog tias nws tso tawm ob peb micrograms. Nrog rau kev tsom mus rau kev tshawb fawb txog kev txhais lus, kev tshawb fawb txog cov qauv thiab kev kho mob rov qab, ntau tus kws tshawb fawb tab tom pib saib "duab loj". Txawm hais tias lawv tab tom sim ntxuav ob peb grams ntawm cov protein rau kev sim ua kom muaj crystallization lossis sim qhov ua tau ntawm kev tsim cov khoom tshiab gene rau hauv cov tshuaj tshiab, cov kws tshawb fawb no sai sai pom lawv tus kheej xav txog qhov nyuaj ntawm kev cog qoob loo loj.

Ua tsaug rau qhov ua tiav ntawm kev lag luam biotechnology, kev nthuav dav ntawm kev cog qoob loo ntawm tes twb yog txoj kev taug kev zoo lawm. "Lub teb chaws twb tawg ntawm cov seams raws li ntau yam khoom tshwm sim, xws li 100 ml conical flasks cog rau hauv shakers mus rau 1,000 L bioreactor kab lis kev cai, nrog rau cov tshuaj muaj peev xwm tsim tau hauv cov hlwb tsiaj txhu hauv ntau qhov ntau.

radobio tuaj yeem muab cov khoom siv shaker zoo heev rau kev cog qoob loo ntawm cov hlwb, thiab hauv lub rooj sib tham no, cov khoom siv shaker tshiab CS345X tau ua pov thawj, uas muaj cov txiaj ntsig zoo li nram no:
❏ Ntau qhov amplitudes hloov kho tau rau ntau yam kev xav tau ntawm cov cell culture.
▸ 12.5/25/50 hli qhov siab hloov kho tau, ib tus tuaj yeem ua tau raws li kev sim kab lis kev cai ntawm tes sib txawv, tsis tas yuav yuav ntau yam khoom siv rau kev xav tau sib txawv, txuag cov neeg siv ntau tus nqi.
❏ Ntau qhov ceev, qeeb qeeb du thiab ruaj khov.
▸ Cov thev naus laus zis tshwj xeeb thiab tshiab ua rau qhov kev tswj ceev dav dua, uas tuaj yeem paub qhov kev tswj ceev ntawm 1 ~ 370rpm, muab kev lees paub zoo kom ua tau raws li kev xav tau sib txawv.
❏ Qhov rooj qhib tau zoo thiab txav tau mus rau sab saud txuag tau qhov chaw thiab yooj yim nkag mus rau cov kab lis kev cai.
▸ Qhov rooj qhib tau zoo dua tsis ua rau lub qhov rooj sab nraud puas, thiab yooj yim nkag mus rau cov kab lis kev cai.
❏ Kev xaiv ua haujlwm tswj cov av noo tuaj yeem tswj cov av noo txog li 90% rh
▸ Rindo lub module tswj cov av noo ua haujlwm ua kom ntseeg tau tias kev tswj cov av noo raug thiab txhim khu kev qha nrog kev ruaj khov ntawm ± 2% rh
❏ Lub tshuab tsav hlau nplaum rau kev ua haujlwm du, siv hluav taws xob tsawg, tiv thaiv ib puag ncig thiab txuag hluav taws xob.
▸ Tsis tas yuav siv siv txoj siv sia, txo qhov kev pheej hmoo ntawm kev ua qias tuaj vim yog cua sov tom qab los ntawm kev sib txhuam ntawm txoj siv sia rau qhov kub thiab txias thiab cov khoom hnav.