03.Agasti 2023 | Ingqungquthela yophuhliso lwe-bioprocesses ye-Biopharmaceutical

Ingqungquthela yophuhliso lwe-bioprocess ye-biopharmaceutical ka-2023,I-radobio ithatha inxaxheba njengomthengisi wenkcubeko yeeseli ze-biopharmaceutical.

Ngokwesiko, ibhayoloji yelabhoratri ibisoloko iyinto encinci; imithambo yokukhulisa izicubu ayinkulu kangako kunesandla somvavanyi, imiqulu ilinganiswa ngee-“milliliters,” kwaye ukucocwa kweproteni kuthathwa njengempumelelo ukuba ivelisa ii-microgram ezimbalwa. Ngokugxila ngakumbi kuphando lokuguqulela, ibhayoloji yolwakhiwo kunye namayeza okuvuselela, izazinzulu ezininzi ziqala ukujonga "umfanekiso omkhulu". Nokuba zizama ukucoca iigram ezimbalwa zeproteni kwiimvavanyo zokwenziwa kwekristale okanye zivavanya ukuba kunokwenzeka na ukuphuhlisa imveliso entsha yejini ibe liyeza elitsha, aba baphandi kungekudala bazifumana becinga ngobunzima benkcubeko enkulu yeseli.

Ngenxa yempumelelo yoshishino lwe-biotechnology, ukwandiswa ngokuthe nkqo kwenkcubeko yeeseli sele kuyindlela ehambe kakuhle. “Eli candelo sele liphuphuma njengoko kuvela uluhlu olubanzi lweemveliso, ukusuka kwiiflaski ze-conical eziyi-100 ml ezikhuliswe kwii-shakers ukuya kwii-bioreactor cultures eziyi-1,000 L, kunye namayeza anokuveliswa kwiiseli zezilwanyana ezincancisayo ngobuninzi.

I-radobio inokubonelela ngeemveliso ezibalaseleyo ze-shaker zokukhulisa iiseli ezixutyiweyo, kwaye kule ngqungquthela, imveliso entsha ye-shaker i-CS345X ibonisiwe, enezibonelelo ezilandelayo:
❏ Ii-amplitudes ezininzi ezihlengahlengiswayo kwiimfuno ezahlukeneyo zenkcubeko yeseli.
▸ I-amplitude ehlengahlengiswayo eyi-12.5/25/50mm, umntu unokuhlangabezana ngempumelelo novavanyo lwenkcubeko yeeseli ezahlukeneyo, ngaphandle kwesidingo sokuthenga izixhobo ezininzi kwiimfuno ezahlukeneyo zovavanyo, okonga abasebenzisi iindleko ezininzi.
❏ Uluhlu olubanzi lwesantya, isantya esiphantsi sigudileyo kwaye isantya esiphezulu sizinzile.
▸ Ubuchwepheshe bokuthwala obukhethekileyo nobutsha bukwandisa uluhlu lolawulo lwesantya, olunokufikelela kuluhlu lolawulo lwesantya oluyi-1 ~ 370rpm, lubonelela ngesiqinisekiso esisebenzayo sokuhlangabezana neemfuno ezahlukeneyo zovavanyo.
❏ Ukutyibilika komnyango ovulekayo phezulu kusindisa indawo kwaye kunika ukufikelela lula kwiinkcubeko.
▸ Ukutyibilika komnyango ovulekayo phezulu kuthintela indawo ehlala umnyango ovulekayo ngaphandle, kwaye kunika ukufikelela okulula kwiinkcubeko.
❏ Umsebenzi wokulawula ukufuma okhethekileyo unokulawula ukufuma ukuya kuthi ga kwi-90%rh
▸ Imodyuli yokulawula ukufuma esebenzayo eyakhelwe ngaphakathi kaRindo iqinisekisa ulawulo lokufuma oluchanekileyo noluthembekileyo oluzinzileyo lwe-±2% rh
❏ I-magnetic drive yokusebenza kakuhle, ukusetyenziswa kwamandla aphantsi, ukhuseleko lokusingqongileyo kunye nokonga amandla.
▸ Akukho mfuneko yeebhanti, nto leyo enciphisa umngcipheko wongcoliseko olubangelwa bubushushu obuvela ekungqubaneni kwebhanti kubushushu bokufukamela kunye neengceba ezigugileyo.