03.Agasti 2023 | Ingqungquthela yokuthuthukiswa kwezinqubo ze-biopharmaceutical bioprocess

Ingqungquthela yokuthuthukiswa kwezinqubo ze-biopharmaceutical bioprocess ka-2023,i-radobio ihlanganyela njengomhlinzeki we-biopharmaceutical cell culture.

Ngokwesiko, i-biology yelabhorethri ibilokhu isebenza ngezinga elincane; imithambo yokukhulisa izicubu ayivamile ukuba nkulu kunesandla somhloli, amavolumu alinganiswa ngama-“milliliters,” futhi ukuhlanzwa kwamaprotheni kubhekwa njengempumelelo uma kukhiqiza ama-microgram ambalwa. Njengoba kugxilwe kakhulu ocwaningweni lokuhumusha, i-biology yesakhiwo kanye nemithi yokuvuselela, ososayensi abaningi baqala ukubheka “isithombe esikhulu”. Kungakhathaliseki ukuthi bazama ukuhlanza amagremu ambalwa eprotheyini ukuze kwenziwe izivivinyo zokwakheka kwamakristalu noma bahlole ukuthi kungenzeka yini ukuthuthukisa umkhiqizo omusha wezakhi zofuzo ube umuthi omusha, laba bacwaningi bazithola sebecabanga ngobunzima besiko lamaseli amakhulu.

Ngenxa yempumelelo yomkhakha we-biotechnology, ukwanda okuqondile kwesiko lamaseli sekuvele kuyindlela ehambe kahle. “Insimu isivele igcwele njengoba kuvela imikhiqizo eminingi, kusukela kumaflaski ayi-100 ml ahlanganiswe ngama-shakers kuya kuma-bioreactor cultures angu-1,000 L, kanti imithi ikwazi ukukhiqizwa kumaseli ezilwane ezincelisayo ngobuningi obukhulu.

I-radobio inganikeza imikhiqizo emihle kakhulu ye-shaker yokukhulisa amaseli okumiswa, futhi kule ngqungquthela, kwaboniswa umkhiqizo omusha we-shaker i-CS345X, onezinzuzo ezilandelayo:
❏ Ama-amplitude amaningi alungisekayo ezidingo ezahlukene zokukhula kwamaseli.
▸ I-amplitude elungisekayo engu-12.5/25/50mm, umuntu angahlangabezana ngempumelelo nokuhlolwa okuhlukile kokukhuliswa kwamaseli, ngaphandle kwesidingo sokuthenga amadivayisi amaningi ngezidingo ezahlukene zokuhlola, okusindisa abasebenzisi izindleko eziningi.
❏ Ibanga lesivinini elibanzi, isivinini esiphansi sibushelelezi futhi isivinini esiphezulu sizinzile.
▸ Ubuchwepheshe bokuthwala obuhlukile nobusha bukhulisa kakhulu ububanzi bokulawula isivinini, obungafinyelela ububanzi bokulawula isivinini obungu-1 ~ 370rpm, buhlinzeka ngesiqinisekiso esisebenzayo sokuhlangabezana nezidingo ezahlukene zokuhlola.
❏ Ukuvula umnyango oshelelayo phezulu kusindisa isikhala futhi kunikeza ukufinyelela okulula ezitshalweni.
▸ Ukuvula umnyango oshelelayo phezulu kugwema isikhala esithathwa umnyango ongaphandle, futhi kunikeza ukufinyelela okulula kakhulu ezitshalweni.
❏ Umsebenzi wokulawula umswakama osebenzayo ongawukhetha ungalawula umswakama ofika ku-90%rh
▸ Imojuli yokulawula umswakama esebenzayo eyakhelwe ngaphakathi kaRindo iqinisekisa ukulawulwa komswakama okunembile nokuthembekile ngokuqina okungu-±2% rh
❏ Idrayivu kamazibuthe yokusebenza kahle, ukusetshenziswa kwamandla aphansi, ukuvikelwa kwemvelo nokonga amandla.
▸ Asikho isidingo samabhande, kunciphisa ingozi yokungcola ngenxa yokushisa kwangemuva okuvela ekungqubuzaneni kwebhande ekushiseni kokushisa kanye nezinhlayiya ezigugile.