Umthelela wokuhlukahluka kwezinga lokushisa kusiko lamaseli
Izinga lokushisa liyipharamitha ebalulekile kusiko lweseli ngoba lithinta ukuphindaphindeka kwemiphumela. Ukushintsha kwezinga lokushisa ngaphezu noma ngaphansi kuka-37°C kunomphumela obaluleke kakhulu ku-kinetics yokukhula kwamaseli amaseli ezincelisayo, afana namaseli ebhaktheriya. Izinguquko ekukhulumeni kofuzo kanye nokuguqulwa kwesakhiwo seselula, ukuqhubeka komjikelezo weseli, ukuzinza kwe-mRNA kungatholwa kumaseli ezincelisayo ngemva kwehora elilodwa ku-32ºC. Ngaphezu kokuthinta ngqo ukukhula kwamaseli, izinguquko kuzinga lokushisa nazo zithinta i-pH yabezindaba, njengoba ukunyibilika kwe-CO2 kushintsha i-pH (i-pH iyanda emazingeni okushisa aphansi). Amaseli ezilwane ezincelisayo akhulisiwe angakwazi ukubekezelela ukwehla kwezinga lokushisa okubalulekile. Angagcinwa ku-4 °C izinsuku ezimbalwa futhi angakwazi ukubekezelela iqhwa ukuya ku -196 °C (kusetshenziswa izimo ezifanele). Kodwa-ke, azikwazi ukubekezelela izinga lokushisa elingaphezu kuka-2 °C ngaphezu kokujwayelekile isikhathi esingaphezu kwamahora ambalwa futhi zizofa ngokushesha ku-40 °C nangaphezulu. Ukuqinisekisa ukukhiqizwa kabusha okuphezulu kwemiphumela, ngisho noma amangqamuzana ephila, kudingeka ukunakekelwa ukuze kugcinwe izinga lokushisa lingaguquki ngangokunokwenzeka ngesikhathi sokufukamela nokuphatha amaseli ngaphandle kwe-incubator.
Izizathu zokuhlukahluka kwezinga lokushisa ngaphakathi kwe-incubator
Uzoqaphela ukuthi lapho umnyango we-incubator uvuleka, izinga lokushisa lehla ngokushesha libe yinani elimisiwe elingu-37 °C. Ngokuvamile, izinga lokushisa lizolulama phakathi nemizuzu embalwa ngemva kokuvalwa komnyango. Eqinisweni, amasiko amile adinga isikhathi sokubuyela ezingeni lokushisa elibekiwe ku-incubator. Izinto eziningi zingathinta isikhathi esisithathayo ukuze isiko lamaseli liphinde lithole izinga lokushisa ngemva kokwelashwa ngaphandle kwe-incubator.Lezi zici zihlanganisa:
- ▶ubude besikhathi amaseli ephumile ku-incubator
- ▶uhlobo lweflask lapho amaseli akhulela khona (ijometri ithinta ukudluliswa kokushisa)
- ▶Inani leziqukathi ku-incubator .
- ▶ Ukuthintana okuqondile kwamaflask neshalofu lensimbi kuthinta ukushintshana kokushisa kanye nesivinini sokufinyelela izinga lokushisa elilungile, ngakho-ke kungcono ukugwema izinqwaba zamaflask nokubeka umkhumbi ngamunye.
- ▶ngokuqondile eshalofini le-incubator.
Izinga lokushisa lokuqala lanoma yiziphi iziqukathi ezintsha nemidiya esetshenzisiwe izophinde ithinte isikhathi esisithathayo ukuze amaseli abe sesimweni sawo esifanele; lapho izinga lokushisa liphansi, kuthatha isikhathi eside.
Uma zonke lezi zici zishintsha ngokuhamba kwesikhathi, zizophinda zikhulise ukuhluka phakathi kokuhlolwa. Kudingeka ukunciphisa lokhu kushintshashintsha kwezinga lokushisa, ngisho noma kungenakwenzeka njalo ukulawula yonke into (ikakhulukazi uma abantu abaningana basebenzisa i-incubator efanayo).
Indlela yokunciphisa ukuhlukahluka kwezinga lokushisa kanye nokunciphisa isikhathi sokubuyisela izinga lokushisa
Ngokushisa kuqala i-medium
Abanye abacwaningi bajwayele ukufudumala amabhodlela aphelele emidiya kubhavu wamanzi ongu-37 °C ukuwaletha kulokhu kushisa ngaphambi kokusetshenziswa. Kungenzeka futhi ukushisa okuphakathi ku-incubator esetshenziselwa ukushisa okuphakathi kuphela hhayi isiko lamaseli, lapho okuphakathi kungafinyelela izinga lokushisa elifanele ngaphandle kokuphazamisa amasiko amaseli kwenye incubator. Kodwa lokhu, ngokwazi kwethu, ngokuvamile akuzona izindleko ezithengekayo.
Ngaphakathi kwe Incubator
Vula umnyango we-incubator kancane ngangokunokwenzeka futhi uvale ngokushesha. Gwema izindawo ezibandayo, ezidala umehluko wokushisa ku-incubator. Shiya isikhala phakathi kwamaflask ukuze umoya ujikeleze. Amashalofu angaphakathi kwe-incubator angakwazi ukubhobozwa. Lokhu kuvumela ukusabalalisa ukushisa okungcono njengoba kuvumela umoya ukuba udlule emigodini. Kodwa-ke, ukuba khona kwezimbobo kungaholela ekuhlukeni ekukhuleni kwamaseli, ngoba kunomehluko wokushisa phakathi kwendawo enezimbobo kanye nendawo ene-meta. Ngalezi zizathu, uma ukuhlola kwakho kudinga ukukhula okufana kakhulu kwesiko leseli, ungabeka amaflaski amasiko ezisekelweni zensimbi ezinezindawo ezincane zokuthintana, ngokuvamile ezingadingeki kusiko lweseli oluvamile.
Ukunciphisa Isikhathi Sokucubungula Iseli
Ukuze unciphise isikhathi esichithwa kunqubo yokwelashwa kwamaseli, udinga ukwenza kanjalo
- ▶Hlela zonke izinto ezidingekayo namathuluzi ngaphambi kokuthi uqale ukusebenza.
- ▶Sebenza ngokushesha nangokushelela, ubuyekeze izindlela zokuhlola kusenesikhathi ukuze imisebenzi yakho ibe yimpinda futhi izenzekele.
- ▶Nciphisa ukuthintana koketshezi ngomoya osendaweni.
- ▶Gcina izinga lokushisa elingaguquki elebhu yesiko lamaseli lapho usebenza khona.
Isikhathi sokuthumela: Aug-18-2023