中文
Umphumela Wokulinganisa Kwezinga Lokushisa kumasiko weseli
Ukushisa kuyipharamitha ebalulekile kumasiko weseli ngoba kuthinta ukudalulwa kwemiphumela. Izinguquko zokushisa ezingenhla noma ngaphansi kwama-37 ° C zinomphumela obaluleke kakhulu kwi-kinetics yokukhula kwamaseli yamaseli e-mammalia, afana nalewa amaseli wegciwane. Izinguquko ezichazayo zohlobo lwezakhi zofuzo kanye nokuguqulwa ngesakhiwo samaselula, ukuthuthuka komjikelezo weseli, ukuqina kwe-MRNA kungatholwa kumaseli we-mammalian ngemuva kwehora elilodwa ku-32ºC. Ngaphezu kokuthinta ngokuqondile ukukhula kwamaselula, izinguquko zokushisa zithinta futhi i-PH yabezindaba, njengoba i-solubity ye-CO2 iguqula amazinga okushisa aphansi). Amangqamuzana e-Mammalian akhuthaziwe angabekezelela izinga lokushisa elibalulekile liyancipha. Zingagcinwa ku-4 ° C izinsuku eziningana futhi zingabekezelela ukuqaqa ku-196 ° C (kusetshenziswa izimo ezifanele). Kodwa-ke, ngeke bakwazi ukubekezelela amazinga okushisa angenhla cishe ngo-2 ° C ngaphezulu okujwayelekile isikhathi esingaphezu kwamahora ambalwa futhi azokufa masinyane ngo-40 ° C nangaphezulu. Ukuqinisekisa ukuvela okuphezulu kwemiphumela, noma ngabe amaseli asindayo, ukunakekelwa kudinga ukuthathwa ukuze kugcinwe izinga lokushisa ngokuqhubekayo ngesikhathi sokufakwa kwezindawo zokufakwa.
Izizathu zokuhluka kokushisa ngaphakathi kwe-incubator
Uzobona ukuthi lapho kuvulwa umnyango we-incubator, izinga lokushisa lehla ngokushesha ngenani elibekiwe lama-37 ° C. Ngokuvamile, izinga lokushisa lizolulama phakathi kwemizuzu embalwa ngemuva kokuthi umnyango uvaliwe. Eqinisweni, amasiko angotshani adinga isikhathi sokululama ekushiseni okusethiwe ku-incubator. Izici eziningi zingathinta isikhathi esithatha isikhathi sesiko leseli ukuthola kabusha izinga lokushisa ngemuva kokwelashwa ngaphandle kwe-incubator.Lezi zinto zifaka:
- ▶ Ubude besikhathi amaseli abephumile ku-incubator
- ▶ Uhlobo lweflask lapho amaseli esekhulile (i-geometry athinta ukudluliswa kokushisa)
- ▶ Inombolo yeziqukathi ku-incubator.
- Ukuthintana okuqondile kwama-flasks ngeshalofu lensimbi kuthinta ukushintshaniswa kokushisa kanye nejubane lokufinyelela okushisa kahle, ngakho-ke kungcono ukugwema izitaki zama-flasks futhi ubeke umkhumbi ngamunye
- ▶ ngqo eshalofini le-incubator.
Ukushisa kokuqala kwanoma yiziphi iziqukathi ezintsha kanye nemidiya esetshenzisiwe kuzothinta nesikhathi esithatha isikhathi sokuba amangqamuzana abe esimweni sawo esifanele; Amazinga okushisa awo ancipha, kuthatha isikhathi eside.
Uma zonke lezi zinto zishintsha ngokuhamba kwesikhathi, zizothuthukisa nokuhlukahluka phakathi kokuhlolwa. Kuyadingeka ukunciphisa lokhu kuguquguquka kwezinga lokushisa, noma ngabe akwenzeki njalo ukulawula konke (ikakhulukazi uma abantu abaningana besebenzisa i-incubator efanayo).
Unganciphisa kanjani ukuhluka kokushisa futhi unciphise isikhathi sokuvuselelwa kwamazinga okushisa
Ngokushisa okuphakathi nendawo
Abanye abacwaningi bajwayele ukufudumeza kwamabhodlela abezindaba ngaphambi kokugeza kwamanzi angama-37 ° C ukuletha kulokhu kushisa ngaphambi kokusebenzisa. Kungenzeka futhi ukuphinda ukhethe okuphakathi kwi-incubator esetshenziselwa kuphela ukusabalalisa okuphakathi hhayi amasiko weseli, lapho i-medium ingafinyelela khona okushisa kahle ngaphandle kokuphazamisa amasiko weseli kwelinye icala. Kepha lokhu, ngokwazi kwethu, ngokuvamile akuyona izindleko ezingabizi.
Ngaphakathi kwe-incubator
Vula umnyango we-incubator kancane ngangokunokwenzeka bese uyivalela ngokushesha. Gwema izindawo ezibandayo, ezakha umehluko wokushisa ku-incubator. Shiya isikhala phakathi kwama-flasks ukuvumela umoya ukujikeleza. Amashalofu ngaphakathi kwe-incubator angaqokwa. Lokhu kuvumela ukusatshalaliswa kokushisa okungcono njengoba kuvumela umoya ukuba udlule ezimbobo. Kodwa-ke, ukuba khona kwezimbobo kungaholela ekwehlukahluka ekukhuleni kwamaseli, ngoba kunomehluko wokushisa phakathi kwendawo enezimbobo nendawo ene-meta. Ngalezi zizathu, uma izivivinyo zakho zidinga ukukhula okufana kakhulu kwesiko leseli, ungabeka amaflasks amasiko ekusekelweni kwensimbi nge-surfari emincane yokuxhumana, okuvame ukungadingeki kusiko lokuxhumana elincanyana.
Ukunciphisa isikhathi sokucubungula iseli
Ukunciphisa isikhathi esisetshenziswa kwinqubo yokwelashwa kweseli, udinga
- ▶ Hlela zonke izinto ezidingekayo namathuluzi ngaphambi kokuqala ukusebenza.
- ▶ Sebenza ngokushesha nangokushelela, ubuyekeze izindlela zokuhlola ngaphambi kwesikhathi ukuze imisebenzi yakho iphindeke futhi isebenze.
- ▶ Nciphisa ukuxhumana koketshezi ngomoya okhona.
- ▶ Gcina izinga lokushisa elihlala njalo kwilebhu yesiko leselula lapho usebenza khona.
Isikhathi sePosi: Aug-18-2023