Kodi mungasankhe bwanji ma amplitude oyenera a shaking incubator?
Kodi mphamvu ya shaker ndi yotani?
Kutalika kwachogwirira choyatsirandi m'mimba mwake wa phale lozungulira, nthawi zina limatchedwa "m'mimba mwake wozungulira" kapena chizindikiro cha "m'mimba mwake wotsatira": Ø. Radobio imapereka ma shakers okhazikika okhala ndi ma amplitude a 3mm, 25mm, 26mm ndi 50mm,. Ma shakers opangidwa mwamakonda okhala ndi ma amplitude ena amapezekanso.
Kodi Mpweya Wosamutsa Mpweya (OTR) ndi Chiyani?
Kuchuluka kwa mpweya woipa (OTR) ndi mphamvu ya mpweya woipa womwe umasamutsidwa kuchokera mumlengalenga kupita ku madzi. Kuchuluka kwa mpweya woipa (OTR) kumatanthauza kuti mpweya woipa umasamutsidwa bwino.
Zotsatira za Kukula ndi Kuthamanga kwa Kuzungulira
Zinthu zonsezi zimakhudza kusakaniza kwa chosungira mu botolo lokulitsa. Kusakaniza bwino, kumaperekanso mpweya wabwino (OTR). Potsatira malangizo awa, mutha kusankha amplitude yoyenera komanso liwiro lozungulira.
Kawirikawiri, kusankha matalikidwe a 25mm kapena 26mm kungagwiritsidwe ntchito ngati matalikidwe apadziko lonse pa ntchito zonse zachitukuko.
Matenda a bakiteriya, yisiti ndi bowa:
Kusamutsa mpweya m'mabotolo otenthetsera sikugwira ntchito bwino kwambiri kuposa m'mabotolo otenthetsera. Kusamutsa mpweya m'mabotolo otenthetsera kungakhale chinthu choletsa kukula kwa mabotolo otenthetsera nthawi zambiri. Kutalika kwake kumakhudzana ndi kukula kwa mabotolo ozungulira: mabotolo akuluakulu amagwiritsa ntchito kukula kwakukulu.
Malangizo: 25mm kutalika kwa mabotolo okhala ndi mawonekedwe ofanana kuyambira 25ml mpaka 2000ml.
Kutalika kwa 50 mm kwa mabotolo ozungulira kuyambira 2000 ml mpaka 5000 ml.
Kuchuluka kwa Maselo:
* Kulima maselo a ziweto za m'nyanja kumafuna mpweya wochepa.
* Pa ma flask a shaker a 250ml, mpweya wokwanira ukhoza kuperekedwa pa ma amplitude ndi liwiro losiyanasiyana (20-50mm amplitude; 100-300rpm).
* Pa ma flask akuluakulu (ma flask a Fernbach) akulimbikitsidwa kuti azitha kukula mpaka 50mm.
* Ngati matumba osungiramo zinthu zotayidwa agwiritsidwa ntchito, kukula kwa 50mm kumalimbikitsidwa.
Ma microtiter ndi mbale zakuya:
Pa ma microtiter ndi ma deep-well plates pali njira ziwiri zosiyana zopezera mpweya wabwino kwambiri!
* 50 mm matalikidwe pa liwiro la osachepera 250 rpm.
* Gwiritsani ntchito matalikidwe a 3mm pa 800-1000rpm.
Nthawi zambiri, ngakhale atasankha kuchuluka koyenera kwa amplitude, sikungawonjezere kuchuluka kwa zamoyo, chifukwa kuwonjezeka kwa kuchuluka kumatha kukhudzidwa ndi zinthu zingapo. Mwachitsanzo, ngati chimodzi kapena ziwiri mwa zinthu khumi sizili bwino, ndiye kuti kuwonjezeka kwa kuchuluka kwa zamoyo kudzachepa mosasamala kanthu kuti zinthu zina zili bwino bwanji, kapena tinganene kuti kusankha kolondola kwa amplitude kudzapangitsa kuti chosungiramo zinthu chiwonjezeke kwambiri ngati chinthu chokhacho choletsa kuchuluka kwa zamoyo ndi mpweya woperekedwa. Mwachitsanzo, ngati gwero la kaboni ndiye chinthu choletsa, ngakhale mpweya utasamutsidwa bwino bwanji, kuchuluka kwa zamoyo zomwe mukufuna sikudzapezeka.
Kuthamanga kwa Makulidwe ndi Kuzungulira
Kuchuluka kwa mpweya ndi liwiro lozungulira zonse ziwiri zimatha kukhudza kusamutsa mpweya. Ngati maselo akukula pa liwiro lozungulira lotsika kwambiri (monga 100 rpm), kusiyana kwa kuchuluka kwa mpweya sikukhudza kwambiri kusamutsa mpweya. Kuti mukwaniritse kusamutsa mpweya kwambiri, gawo loyamba ndikuwonjezera liwiro lozungulira momwe mungathere, ndipo thireyi idzakhala yolinganizidwa bwino kuti igwirizane ndi liwiro. Si maselo onse omwe amatha kukula bwino ndi kugwedezeka kwa liwiro lalikulu, ndipo maselo ena omwe amakhudzidwa ndi mphamvu zodula amatha kufa chifukwa cha liwiro lalikulu lozungulira.
Zina zomwe zimayambitsa
Zinthu zina zitha kukhala ndi zotsatira pa kusamutsa mpweya:
* Kuchuluka kwa zodzaza, mabotolo okhala ndi mawonekedwe ofanana sayenera kudzazidwa mpaka gawo limodzi mwa magawo atatu a kuchuluka konse. Ngati mukufuna kuti mpweya ufike pamlingo waukulu, mudzaze mpaka 10%. Musadzaze mpaka 50%.
* Zopopera: Zopopera zimathandiza kwambiri pakupititsa patsogolo kufalikira kwa mpweya m'mitundu yonse ya zomera. Opanga ena amalimbikitsa kugwiritsa ntchito mabotolo a "Ultra High Yield". Zopopera zomwe zili pamabotolo awa zimawonjezera kukangana kwamadzimadzi ndipo chogwedeza sichingafikire liwiro lalikulu lomwe lakhazikitsidwa.
Kugwirizana pakati pa kukula ndi liwiro
Mphamvu ya centrifugal mu shaker ikhoza kuwerengedwa pogwiritsa ntchito equation yotsatirayi
FC = rpm2× kukula
Pali ubale wolunjika pakati pa mphamvu ya centrifugal ndi amplitude: ngati mugwiritsa ntchito amplitude ya 25 mm mpaka amplitude ya 50 mm (pa liwiro lomwelo), mphamvu ya centrifugal imawonjezeka ndi 2.
Pali ubale wapakati pakati pa mphamvu ya centrifugal ndi liwiro lozungulira.
Ngati liwiro lawonjezeka ndi 2 (amplitude yomweyo), mphamvu ya centrifugal imawonjezeka ndi 4. Ngati liwiro lawonjezeka ndi 3, mphamvu ya centrifugal imawonjezeka ndi 9!
Ngati mugwiritsa ntchito kutalika kwa 25 mm, ikani pa liwiro loperekedwa. Ngati mukufuna kukwaniritsa mphamvu yofanana ya centrifugal ndi kukula kwa 50 mm, liwiro lozungulira liyenera kuwerengedwa ngati muzu wa sikweya wa 1/2, kotero muyenera kugwiritsa ntchito 70% ya liwiro lozungulira kuti mukwaniritse mikhalidwe yofanana ya incubation.
Dziwani kuti zomwe zili pamwambapa ndi njira yongoganizira chabe yowerengera mphamvu ya centrifugal. Pali zinthu zina zomwe zimakhudza momwe zinthu zilili. Njira iyi yowerengera imapereka ma values oyerekeza kuti agwiritsidwe ntchito.
Nthawi yotumizira: Januwale-03-2024