Ungayikhetha kanjani i-amplitude efanele ye-incubator enyakazayo?
Iyini ubukhulu be-shaker?
Ubukhulu be-amplitudeumshini wokufukula i-incubatorububanzi bephalethi elijikelezayo, ngezinye izikhathi elibizwa ngokuthi “ububanzi bokunyakaza” noma “ububanzi bomkhondo”: Ø. I-Radobio inikeza ama-shakers ajwayelekile ane-amplitude engu-3mm, 25mm, 26mm kanye no-50mm. Ama-shakers enziwe ngokwezifiso anobukhulu obunye be-amplitude nawo ayatholakala.
Iyini izinga lokudlulisa umoya-mpilo (i-OTR)?
Izinga Lokudlulisa I-Oxygen (OTR) liwukusebenza kahle kwe-oxygen edluliselwa kusuka emoyeni iye koketshezi. Uma inani le-OTR liphezulu kusho ukuthi ukusebenza kahle kokudlulisa i-oxygen kuphezulu.
Umphumela Wokuphakama Nesivinini Sokujikeleza
Zombili lezi zici zithinta ukuxubana kwe-medium ebhodleleni lokukhulisa. Uma ukuxubana kungcono, izinga lokudlulisa umoya-mpilo (OTR) lingcono. Ngokulandela lezi ziqondiso, i-amplitude kanye nesivinini sokujikeleza esifaneleka kakhulu kungakhethwa.
Ngokuvamile, ukukhetha i-amplitude engu-25mm noma engu-26mm kungasetshenziswa njenge-amplitude yendawo yonke kuzo zonke izinhlelo zokusebenza zokukhuliswa.
Amasiko amagciwane, imvubelo kanye nesikhunta:
Ukudluliselwa komoya-mpilo kumaflaski okushukuma akusebenzi kahle kakhulu kunakuma-bioreactor. Ukudluliselwa komoya-mpilo kungaba yisici esivimbela ukukhula kwamaflaski okushukuma ezimweni eziningi. Ukuphakama kuhlobene nosayizi wamaflaski ayindilinga: amaflaski amakhulu asebenzisa ama-amplitude amakhulu.
Isincomo: ubukhulu obungu-25mm bamaflaski anombala ofana nowekhoni kusukela ku-25ml kuya ku-2000ml.
Ububanzi obungu-50 mm bamaflaski anombala ofana nowekhoni busukela ku-2000 ml kuya ku-5000 ml.
Ukukhula Kwamaseli:
* Ukukhula kwamaseli ezilwane ezincelisayo kunesidingo esincane se-oxygen.
* Kumaflaski okushisa angu-250mL, ukulethwa komoya-mpilo okwanele kunganikezwa ngebanga elibanzi lama-amplitude kanye nesivinini (20-50mm amplitude; 100-300rpm).
* Kumaflaski amakhulu (amaflaski aseFernbach) kunconywa ubukhulu obungu-50mm.
* Uma kusetshenziswa izikhwama zokukhulisa ezilahlwayo, kunconywa ubukhulu obungu-50mm.
Amapuleti e-Microtiter kanye ne-deep-well:
Kuma-microtiter nama-deep-well plate kunezindlela ezimbili ezahlukene zokuthola ukudluliswa komoya-mpilo okuphezulu kakhulu!
* 50 mm amplitude ngesivinini esingaphansi kuka-250 rpm.
* Sebenzisa ubukhulu obungu-3mm ku-800-1000rpm.
Ezimweni eziningi, noma ngabe kukhethwa i-amplitude efanelekile, ingase ingakhulisi ivolumu ye-bioculture, ngoba ukwanda kwevolumu kungathonywa yizici eziningana. Isibonelo, uma eyodwa noma ezimbili zezici eziyishumi zingalungile, khona-ke ukwanda kwevolumu ye-culture kuzonqunyelwa kungakhathaliseki ukuthi ezinye izici zinhle kangakanani, noma kungaphikiswana ngokuthi ukukhetha okufanele kwe-amplitude kuzoholela ekwandeni okubonakalayo kwe-incubator uma okuwukuphela kwesici esikhawulela ivolumu ye-culture ukulethwa kwe-oxygen. Isibonelo, uma umthombo we-carbon uyisici esikhawulela, kungakhathaliseki ukuthi ukudluliselwa kwe-oxygen kuhle kangakanani, ivolumu ye-culture oyifunayo ngeke ifinyelelwe.
Isivinini Sokuphakama Nokujikeleza
Kokubili ubukhulu kanye nesivinini sokujikeleza kungaba nomthelela ekudlulisweni komoya-mpilo. Uma ukukhuliswa kwamaseli kukhuliswa ngesivinini sokujikeleza esiphansi kakhulu (isb., i-100 rpm), umehluko kubukhulu awunawo umphumela obonakalayo ekudlulisweni komoya-mpilo. Ukuze kufezwe ukudluliswa komoya-mpilo okuphezulu kakhulu, isinyathelo sokuqala ukwandisa isivinini sokujikeleza ngangokunokwenzeka, futhi ithreyi izolinganiswa kahle ngesivinini. Akuwona wonke amaseli angakhula kahle ngokuzulazula kwesivinini esikhulu, futhi amanye amaseli azwela amandla okusika angase afe ngenxa yesivinini sokujikeleza esiphezulu.
Amanye amathonya
Ezinye izici zingaba nomthelela ekudlulisweni komoya-mpilo:.
* Umthamo wokugcwalisa, amaflaski ayindilinga kufanele agcwaliswe kungabi ngaphezu kwengxenye yesithathu yomthamo ophelele. Uma kuzofinyelelwa ukudluliswa komoya-mpilo okuphezulu, gcwalisa kungabi ngaphezu kwe-10%. Ungalokothi ugcwalise kungabi ngaphezu kwe-50%.
* Ama-Spoiler: Ama-Spoiler asebenza kahle ekuthuthukiseni ukudluliswa komoya-mpilo kuzo zonke izinhlobo zezimila. Abanye abakhiqizi batusa ukusetshenziswa kwama-flask “e-Ultra High Yield”. Ama-spoiler kula ma-flask andisa ukungqubuzana koketshezi futhi i-shaker ingase ingafinyeleli isivinini esiphezulu esibekiwe.
Ubudlelwano phakathi kwe-amplitude kanye nesivinini
Amandla e-centrifugal ku-shaker angabalwa kusetshenziswa i-equation elandelayo
I-FC = i-rpm2× ubukhulu
Kukhona ubudlelwano obuqondile phakathi kwamandla e-centrifugal kanye ne-amplitude: uma usebenzisa i-amplitude engu-25 mm kuya ku-amplitude engu-50 mm (ngesivinini esifanayo), amandla e-centrifugal anda ngesilinganiso esingu-2.
Kukhona ubudlelwano obuyisikwele phakathi kwamandla e-centrifugal kanye nesivinini sokujikeleza.
Uma ijubane likhushulwa ngesilinganiso esingu-2 (i-amplitude efanayo), amandla e-centrifugal anda ngesilinganiso esingu-4. Uma ijubane likhushulwa ngesilinganiso esingu-3, amandla e-centrifugal anda ngesilinganiso esingu-9!
Uma usebenzisa ubukhulu obungu-25 mm, fukamela ngesivinini esithile. Uma ufisa ukufeza amandla afanayo e-centrifugal nge-ububude obungu-50 mm, isivinini sokujikeleza kufanele sibalwe njengempande yesikwele engu-1/2, ngakho-ke kufanele usebenzise u-70% wejubane lokujikeleza ukuze ufeze izimo ezifanayo zokufukamela.
Sicela uqaphele ukuthi okungenhla kuyindlela nje yemfundiso yokubala amandla e-centrifugal. Kunezinye izici ezithonyayo ekusetshenzisweni kwangempela. Le ndlela yokubala inikeza amanani alinganiselwe ngezinjongo zokusebenza.
Isikhathi sokuthunyelwe: Jan-03-2024