Ungayikhetha njani i-amplitude efanelekileyo ye-incubator eshukumayo?
Ithini i-amplitude ye-shaker?
Ubungakanani be-amplitudeisihluzi se-incubatorlububanzi bepalethi ejikelezayo, ngamanye amaxesha ebizwa ngokuba yi-"oscillation diameter" okanye "track diameter": Ø. I-Radobio inikezela ngee-shakers ezisemgangathweni ezine-amplitude ye-3mm, 25mm, 26mm kunye ne-50mm. Ii-shakers ezenziwe ngokwezifiso ezinezinye ii-amplitude sizes nazo ziyafumaneka.
Yintoni i-Oxygen Transfer Rate (OTR)?
Izinga lokuThunyelwa kweOksijini (i-OTR) kukusebenza kakuhle kweoksijini xa idluliselwa ukusuka emoyeni ukuya kulwelo. Okukhona ixabiso le-OTR liphezulu kuthetha ukuba ukusebenza kakuhle kweoksijini kuphezulu.
Isiphumo se-Amplitude kunye ne-Rotation Speed
Zombini ezi zinto zichaphazela ukuxutywa kwesixhobo esikwiflaski yokukhulisa izityalo. Okukhona ukuxutywa kulunge ngakumbi, kokukhona izinga lokudluliselwa kweoksijini (OTR) lingcono. Ukulandela le migaqo, i-amplitude kunye nesantya sokujikeleza esifanelekileyo sinokukhethwa.
Ngokubanzi, ukukhetha i-amplitude engama-25mm okanye engama-26mm kungasetyenziswa njenge-amplitude eqhelekileyo kuzo zonke izicelo zenkcubeko.
Iinkcubeko zebhaktiriya, igwele kunye nefungus:
Ukudluliselwa kweoksijini kwiiflaski ezishukumayo akusebenzi kakuhle kakhulu kunakwii-bioreactors. Ukudluliselwa kweoksijini kunokuba yinto ethintela ukukhula kweeflaski ezishukumayo kwiimeko ezininzi. Ubukhulu be-amplitude bunxulumene nobukhulu beeflaski ezijijekileyo: iiflaski ezinkulu zisebenzisa ubukhulu be-amplitude.
Ingcebiso: ubukhulu be-25mm kwiiflaski ezijijekileyo ukusuka kwi-25ml ukuya kwi-2000ml.
Ubukhulu obuyi-50 mm kwiiflaski ezijijekileyo ukusuka kwi-2000 ml ukuya kwi-5000 ml.
Inkcubeko yamaSeli:
* Inkcubeko yeeseli zezilwanyana ezincelisayo ayifuni oksijini ininzi.
* Kwiiflaski ze-shaker ezingama-250mL, ukuhanjiswa kweoksijini eyaneleyo kunokunikezelwa kuluhlu olubanzi lwee-amplitude kunye nesantya (20-50mm amplitude; 100-300rpm).
* Kwiiflaski ezinkulu (iiflaski zaseFernbach) kuyacetyiswa ukuba kubekho ubukhulu obuyi-50mm.
* Ukuba kusetyenziswa iingxowa zokukhulisa ezilahlwayo, kucetyiswa ubukhulu obuyi-50mm.
Iiplate zeMicrotiter kunye ne-deep-well:
Kwi-microtiter kunye neeplates ezinzulu kukho iindlela ezimbini ezahlukeneyo zokufumana udluliselo lweoksijini oluphezulu!
* Ubukhulu be-50 mm ngesantya esingaphantsi kwe-250 rpm.
* Sebenzisa ubukhulu be-3mm kwi-800-1000rpm.
Kwiimeko ezininzi, nokuba kukhethwe i-amplitude efanelekileyo, isenokunganyusi i-bioculture volume, kuba ukunyuka kwe-volume kunokuchatshazelwa zizinto ezininzi. Umzekelo, ukuba enye okanye ezimbini kwezilishumi azilunganga, ngoko ukunyuka kwe-culture volume kuya kuncitshiswa nokuba ezinye izinto zilungile kangakanani na, okanye kunokuthiwa ukhetho oluchanekileyo lwe-amplitude luya kubangela ukwanda okubonakalayo kwi-incubator ukuba into ekuphela kwayo ethintela i-culture volume kukuhanjiswa kwe-oxygen. Umzekelo, ukuba umthombo wekhabhoni yi-limiting factor, nokuba i-oxygen transfer ilungile kangakanani na, i-culture volume oyifunayo ayiyi kufezekiswa.
Ububanzi kunye nesantya sokujikelezisa
Zombini i-amplitude kunye nesantya sokujikeleza zinokuba nefuthe ekudlulisweni kweoksijini. Ukuba ii-cell cultures zikhuliswe ngesantya esiphantsi kakhulu sokujikeleza (umz., i-100 rpm), umahluko kwi-amplitude awunampembelelo ingako okanye awubonakali kwaphela ekudlulisweni kweoksijini. Ukuze kufezekiswe udluliselo lweoksijini oluphezulu, inyathelo lokuqala kukunyusa isantya sokujikeleza kangangoko kunokwenzeka, kwaye itreyi iya kulungelelaniswa ngokufanelekileyo ngesantya. Ayizizo zonke iiseli ezinokukhula kakuhle ngesantya esiphezulu sokujikeleza, kwaye ezinye iiseli ezibuthathaka kumandla okucheba zinokufa ngenxa yesantya esiphezulu sokujikeleza.
Ezinye iimpembelelo
Ezinye izinto zinokuba nefuthe ekudlulisweni kweoksijini:.
* Umthamo wokuzalisa, iiflaski ezijiyileyo kufuneka zizaliswe zingadluli kwisithathu sesithathu somthamo uwonke. Ukuba kufuneka kufezekiswe udluliselo lweoksijini oluphezulu, zizalise zingadluli kwi-10%. Ungaze uzalise ungadluli kwi-50%.
* IiSpoilers: IiSpoilers zisebenza kakuhle ekuphuculeni ukudluliselwa kweoksijini kuzo zonke iintlobo zezityalo. Abanye abavelisi bacebisa ukusetyenziswa kweeflaski ze-“Ultra High Yield”. IiSpoilers ezikwezi flaski zonyusa ukungqubana kolwelo kwaye i-shaker isenokungafikeleli kwisantya esiphezulu esimiselweyo.
Ulwalamano phakathi kwe-amplitude kunye nesantya
Amandla e-centrifugal kwi-shaker angabalwa kusetyenziswa le equation ilandelayo
I-FC = i-rpm2× ubukhulu
Kukho ulwalamano oluhambelanayo phakathi kwamandla e-centrifugal kunye ne-amplitude: ukuba usebenzisa i-amplitude engama-25 mm ukuya kwi-amplitude engama-50 mm (ngesantya esifanayo), amandla e-centrifugal ayanda nge-factor ye-2.
Kukho ubudlelwane besikwere phakathi kwamandla e-centrifugal kunye nesantya sokujikeleza.
Ukuba isantya sinyuswa nge-factor ye-2 (i-amplitude efanayo), amandla e-centrifugal ayanda nge-factor ye-4. Ukuba isantya sinyuswa nge-factor ye-3, amandla e-centrifugal ayanda nge-factor ye-9!
Ukuba usebenzisa i-amplitude engama-25 mm, yifunxe ngesantya esithile. Ukuba unqwenela ukufikelela kwi-centrifugal force efanayo ne-amplitude engama-50 mm, isantya sokujikeleza kufuneka sibalwe njenge-square root ye-1/2, ngoko ke kufuneka usebenzise i-70% yesantya sokujikeleza ukuze ufikelele kwi-incubation efanayo.
Nceda uqaphele ukuba oku kungasentla yindlela nje yethiyori yokubala amandla e-centrifugal. Kukho ezinye izinto ezinefuthe kwizicelo zokwenyani. Le ndlela yokubala inika amaxabiso aqikelelweyo ngeenjongo zokusebenza.
Ixesha leposi: Jan-03-2024