Nzira yekusarudza amplitude chaiyo ye shaking incubator?
Chii chinonzi amplitude ye shaker?
Kukura kwechinonyungudutsa chinogara vanandiyo dhayamita yepallet iri kufamba nedenderedzwa, dzimwe nguva inonzi chiratidzo che "oscillation diameter" kana "track diameter": Ø. Radobio inopa ma "standard shakers" ane amplitude ye3mm, 25mm, 26mm uye 50mm. Ma "shakers" akagadzirwa nemaoko ane mamwe ma "amplitude" anowanikwawo.
Chii chinonzi Oxygen Transfer Rate (OTR)?
Mwero weKutamisa Okisijeni (OTR) zvinoreva kushanda zvakanaka kweokisijeni kubva mumhepo kuenda mumvura. Kana OTR yakakwira zvinoreva kuti kushanda zvakanaka kweokisijeni.
Mhedzisiro yeAmplitude uye Kumhanya kweKutenderera
Zvinhu izvi zvose zvinokanganisa kusanganiswa kwechinhu chiri mubhodhoro rekurima. Kana kusanganiswa kwacho kuri nani, chiyero chekufambisa mweya (OTR) chinowedzera kunaka. Kutevera nhungamiro idzi, amplitude uye rotational speed zvinokwanisa kusarudzwa.
Kazhinji, kusarudza amplitude ye25mm kana 26mm kunogona kushandiswa seamplitude yepasi rose kune ese mashandisirwo ekurima.
Mabhakitiriya, mbiriso uye fungi tsika:
Kutamiswa kweokisijeni mumaflask ekuzunguza hakunyatsoshanda zvakanaka pane muma bioreactors. Kutamiswa kweokisijeni kungave kuri chinhu chinotadzisa kukura kwemaflask ekuzunguza muzviitiko zvakawanda. Kukura kwacho kwakabatana nehukuru hweflask dzakaita seconical: maflask makuru anoshandisa amplitude yakakura.
Zvinokurudzirwa: 25mm amplitude yemaflasks ane denderedzwa kubva pa25ml kusvika 2000ml.
Hurefu hwe50 mm hwemaflasks ane denderedzwa kubva pa2000 ml kusvika 5000 ml.
Kukura Kwemasero:
* Kukura kwemasero emhuka dzinoyamwisa hakudi okisijeni yakawanda.
* Kune maflask e shaker e 250mL, kuendeswa kweokisijeni yakakwana kunogona kupihwa pamusoro pemhando dzakasiyana-siyana dze amplitude uye kumhanya (20-50mm amplitude; 100-300rpm).
* Kune maflask akakura (maflask eFernbach) amplitude ye50mm inokurudzirwa.
* Kana mabhegi ekurima anoraswa akashandiswa, amplitude ye50mm inokurudzirwa.
Mapureti eMicrotiter neakadzika-tsime:
Kune ma microtiter nema deep-well plates kune nzira mbiri dzakasiyana dzekuwana oxygen transfer yakanyanya!
* 50 mm amplitude nekumhanya kweasingasviki 250 rpm.
* Shandisa 3mm amplitude pa800-1000rpm.
Muzviitiko zvakawanda, kunyangwe kana amplitude yakakodzera ikasarudzwa, inogona kusawedzera huwandu hwekurima, nekuti kuwedzera kwehuwandu kunogona kukanganiswa nezvinhu zvakati wandei. Semuenzaniso, kana chimwe kana zviviri zvezvinhu gumi zvisina kunaka, kuwedzera kwehuwandu hwekurima kuchave kwakaganhurirwa zvisinei nekuti zvimwe zvinhu zvakanaka sei, kana zvinogona kutaurwa kuti kusarudza kwakakodzera kweamplitude kuchaita kuti incubator iwedzere zvakanyanya kana chinhu chega chinogumira huwandu hwekurima chiri kuendeswa kweokisijeni. Semuenzaniso, kana kabhoni iriyo inogumira, zvisinei nekuti kufambiswa kweokisijeni kwakanaka sei, huwandu hwekurima hunodiwa hahuzowanikwe.
Kumhanya kweAmplitude uye Kutenderera
Kumhanya kweamplitude nekumhanya kweamplitude zvinogona kukanganisa kutamiswa kweokisijeni. Kana masero akakura nekumhanya kwakaderera (semuenzaniso, 100 rpm), musiyano weamplitude hauna kana kuti hauna kana mhedzisiro inooneka pakufambiswa kweokisijeni. Kuti uwane kutamiswa kweokisijeni kwakanyanya, danho rekutanga nderekuwedzera kumhanya kweamplitude zvakanyanya sezvinobvira, uye tireyi ichave yakaenzana zvakanaka nekumhanya. Haasi masero ese anogona kukura zvakanaka nekumhanya kweamplitude, uye mamwe masero anonzwa kuchekwa kweamplitude anogona kufa nekumhanya kweamplitude kwakanyanya.
Zvimwe zvinopesvedzera
Zvimwe zvinhu zvinogona kukanganisa kutamiswa kweokisijeni mumuviri:
* Kuzadza, maflasks ane denderedzwa remakona haafanire kuzadzwa kusvika pachikamu chimwe muzvitatu chehuwandu hwese. Kana uchida kufambiswa kweokisijeni zvakanyanya, zadza kusvika paasingasviki 10%. Usambofa wazara kusvika pa50%.
* Zvidhori: Zvidhori zvinoshanda mukuvandudza kutapurirana kweokisijeni mumhando dzakasiyana dzezvirimwa. Vamwe vagadziri vanokurudzira kushandiswa kwemaflaski e "Ultra High Yield". Zvidhori zviri pamaflaski aya zvinowedzera kukweshana kwemvura uye chidhori chinogona kusasvika kumhanya kwakanyanya.
Kubatana pakati pehukuru nekumhanya
Simba re centrifugal riri mu shaker rinogona kuverengwa uchishandisa equation inotevera
FC = rpm2× kukura
Pane hukama hwakatsetseka pakati pesimba repakati nepakati nepakati: kana ukashandisa amplitude ye25 mm kusvika paamplitude ye50 mm (nekumhanya kumwe chete), simba repakati nepakati rinowedzera ne2 factor.
Pane hukama hwakaenzana pakati pesimba re centrifugal nekumhanya kwekutenderera.
Kana kumhanya kwacho kwakawedzerwa nechinhu che2 (same amplitude), simba recentrifugal rinowedzera nechinhu che4. Kana kumhanya kwacho kwakawedzerwa nechinhu che3, simba recentrifugal rinowedzera nechinhu che9!
Kana ukashandisa amplitude ye25 mm, idyara nekumhanya kwakatarwa. Kana uchida kuwana simba re centrifugal rakafanana ne amplitude ye50 mm, kumhanya kwekutenderera kunofanirwa kuverengerwa se square root ye 1/2, saka unofanirwa kushandisa 70% yekumhanya kwekutenderera kuti uwane mamiriro akafanana ekudzvanya.
Ndapota cherechedzai kuti zviri pamusoro apa ingori nzira yekufungidzira yekuverenga simba repakati nepakati. Kune zvimwe zvinhu zvinopesvedzera mukushandiswa chaiko. Nzira iyi yekuverenga inopa hunhu hwekufungidzira hwezvinangwa zvekushanda.
Nguva yekutumira: Ndira-03-2024