Imikoreshereze ya shaking incubator mu miterere y'uturemangingo tw'ibinyabuzima

Umuco w’ibinyabuzima ugabanyijemo ibice bibiri: static culture na shaking culture. Shaking culture, izwi kandi nka suspension culture, ni uburyo bwo gukusanya aho uturemangingo twa mikorobe duterwa mu buryo bw’amazi tugashyirwa kuicyuma gishikuzacyangwa oscillator yo guhindagurika mu buryo buhoraho. Ikoreshwa cyane mu gusuzuma ubwoko bw'imikorobe no mu kwagura mikorobe, kandi ni uburyo bukoreshwa cyane mu gukwirakwiza mu mikorobe, mu buhanga bwa biochimie, mu gushyushya no mu bundi bushakashatsi bwa siyansi y'ubuzima. Gukwirakwiza mu mikorobe ntibikwiriye mu gukwirakwiza ibintu birimo ibinyabutabire bihindagurika, imyuka iturika nkeya n'imyuka idashya cyane ndetse n'ibintu byangiza.

Ni irihe tandukaniro riri hagati y'imico idahinduka n'ihindagurika?

icyuma gishyushya

Icubator ya CO2 yigana ahantu heza ho gukurira uturemangingo, harimo ubushyuhe, ubwinshi bwa CO2 n'ubushuhe n'ibindi bintu byo hanze. Iyo uturemangingo tw’ingirabuzimafatizo duhingwa mu bihe bidahinduka, uturemangingo twihambira ku rukuta rwo hasi rw'icupa maze hakavuka ubwinshi bwa ogisijeni n'intungamubiri bishonga. Ariko, uturemangingo two guhagarara mu bihe byo gukuriraho dukuraho ubwinshi bwa ogisijeni ishonga, ibyo bikaba byiza cyane mu gukura. Mu miterere ya bagiteri n'uturemangingo, ubwinshi bwa ogisijeni butuma habaho imikoranire n'ibice by'ingirabuzimafatizo n'umwuka wa ogisijeni, cyane cyane ku bihumyo, hatabayeho hyphae cyangwa amatsinda. Mycobacteria iboneka mu miterere idahinduka y'ibimera ishobora kugaragara neza ni mycelium, imiterere n'ikura ry'isahani ku miterere y'ibindi bisa; kandi ubwinshi bwa ogisijeni buboneka kuri bagiteri ni uruziga, ni ukuvuga mycelium ikusanyirijwe hamwe mu matsinda. Kubwibyo, mu nganda za mikorobe zifite ingaruka zimwe zo gukuriraho, ubwinshi bwa ogisijeni bukoreshwa cyane. Uburyo bwo gukuriraho buzunguruka mu miterere y'uturemangingo nabwo ni ubwoko bw'ubwinshi bwa ogisijeni ishonga.

Uruhare rw'umuco uhindagurika:

1. Gutwara ibintu byinshi, substrate cyangwa metabolite birushaho gutwara ibintu neza kandi bigira uruhare mu mikorere y'umubiri.

2. Ogisijeni yashongeshejwe, mu gikorwa cyo gukurura umwuka, umwuka urafunguka, bityo binyuze mu guhindagurika kw'umwuka bishobora gutuma umwuka mwinshi wa ogisijeni ushongeshejwe mu buryo bwo gukurura umwuka.

3. guhuza sisitemu, bifasha mu gupima no kumenya ibipimo bitandukanye.