Ukusetyenziswa kwe-shaking incubator kwinkcubeko yeeseli zebhayoloji

Inkcubeko yebhayoloji yahlulwe yaba yinkcubeko engashukumiyo kunye nenkcubeko yokushukuma. Inkcubeko yokushukuma, eyaziwa ngokuba yinkcubeko yokuxhoma, yindlela yokukhulisa apho iiseli ze-microbial zigonyelwa kwindawo engamanzi zize zibekwe kwindawoisishukumisiokanye i-oscillator yokujikeleza rhoqo. Isetyenziswa kakhulu ekuhlolweni koxinzelelo kunye nenkcubeko yokwandiswa kweentsholongwane, kwaye yindlela yokukhulisa esetyenziswa kakhulu kwi-physiology yeentsholongwane, i-biochemistry, ukubila kunye nezinye iinkalo zophando lwesayensi yobomi. Inkcubeko yokushukuma ayifanelekanga kwinkcubeko yezinto eziqulethe izinyibilikisi zeekhemikhali eziguquguqukayo, uxinzelelo oluphantsi lweegesi eziqhumayo kunye neegesi ezitsha kancinci kunye nezinto ezinobuthi.

Yintoni umahluko phakathi kweenkcubeko ezinganyakaziyo nezishukumayo?

i-incubator eshukumisayo

I-CO2 incubator ilinganisa indawo efanelekileyo yokukhulisa iiseli, kubandakanya ubushushu, uxinano lwe-CO2 kunye nokufuma kunye nezinye iimeko zangaphandle. Ukuba iiseli zesiqu zikhuliswe phantsi kweemeko ezizinzileyo, iiseli zinamathela eludongeni olusezantsi lweflaski kwaye kwenziwa i-gradient yoxinano lwe-oksijini enyibilikisiweyo kunye nezondlo. Nangona kunjalo, iiseli ezixhonyiweyo kwiimeko zokukhulisa ezithambileyo zisusa i-gradient yoxinano kwaye zonyusa uxinano lwe-oksijini enyibilikisiweyo, nto leyo elungele ukukhula. Kwiinkcubeko zebhaktheriya kunye neeseli, inkcubeko yokushukumisa iphucula unxibelelwano kunye nezixhobo zemidiya kunye nobonelelo lwe-oksijini, ngakumbi kwifungi, ngaphandle kokwenziwa kwe-hyphae okanye amaqela. I-Mycobacteria efunyenwe kwinkcubeko ezinzileyo yesikhunta inokubonwa ngokucacileyo yi-mycelium, i-morphology kunye nokukhula kwepleyiti kwimeko yezinye ezifanayo; kwaye inkcubeko yokushukumisa efunyenwe yibhaktheriya iyingqukuva, oko kukuthi, i-mycelium idityaniswe kwiqela. Ke ngoko, kushishino lweentsholongwane olunefuthe elifanayo lenkcubeko yokudlidliza, inkcubeko yokushukumisa isetyenzisiwe kakhulu. Indlela yokukhulisa izicubu kwinkcubeko yezicubu ikwaluhlobo lwenkcubeko yokushukumisa.

Indima yenkcubeko eshukumayo:

1. ukudluliselwa kobunzima, i-substrate okanye i-metabolite idluliselwa ngcono kwaye idlala indima kwinkqubo.

2. ioksijini enyibilikisiweyo, kwinkqubo yokukhulisa umoya nge-aerobic, umoya uyahluzwa uvulekile, ngoko ke ngokusebenzisa ukushukuma kunokwenza umoya ongakumbi unyibilikiswe kwindawo yokukhulisa umoya.

3. ukufana kwenkqubo, okuncedayo ekuthathweni kwesampulu nasekumiselweni kweeparameter ezahlukeneyo.