Ukusetshenziswa kwe-shaking incubator ekukhuleni kwamaseli ebhayoloji

Isiko lezinto eziphilayo lihlukaniswe nge-static culture kanye nesiko lokushaqisa. Isiko lokushaqisa, elaziwa nangokuthi isiko lokumisa, liyindlela yokukhulisa lapho amaseli amancane efakwa khona emgodini omanzi bese ebekwa endaweni emanzi.isishaqinoma i-oscillator yokunyakaza okuqhubekayo. Isetshenziswa kabanzi ekuhlolweni kokucindezeleka kanye nokukhuliswa kwamagciwane, futhi iyindlela yokukhulisa esetshenziswa kakhulu kwi-physiology yamagciwane, i-biochemistry, ukubilisa kanye neminye imikhakha yocwaningo lwesayensi yokuphila. Isiko lokunyakazisa alifanele isiko lezinto eziqukethe izinyibilikisi zamakhemikhali eziguquguqukayo, ukugcwala okuphansi kwamagesi aqhumayo kanye namagesi angashisi kakhulu kanye nezinto ezinobuthi.

Uyini umehluko phakathi kwamasiko aguquguqukayo nashukumayo?

i-incubator ethuthumelayo

I-CO2 incubator ilingisa indawo efanele yokukhulisa amaseli, okuhlanganisa izinga lokushisa, ukuhlushwa kwe-CO2 kanye nomswakama kanye nezinye izimo zangaphandle. Uma amaseli okuqala ekhuliswa ngaphansi kwezimo ezimile, amaseli anamathela odongeni olungezansi lweflaski bese kwakheka i-gradient yokuhlushwa kwe-oxygen encibilikisiwe kanye nezakhamzimba. Kodwa-ke, amaseli okumiswa ezimweni zokukhula okumile okunyakazisayo asusa i-gradient yokuhlushwa futhi andise ukuhlushwa kwe-oxygen encibilikisiwe, okuyinto elungele ukukhula. Ezindaweni zamagciwane kanye namaseli, isiko lokunyakazisa sithuthukisa ukuxhumana nezingxenye zemidiya kanye nokuhlinzekwa kwe-oxygen, ikakhulukazi kuma-fungus, ngaphandle kokwakheka kwe-hyphae noma amaqoqo. Ama-Mycobacteria atholakala esikweni esimile sesikhunta angabonakala ngokucacile yi-mycelium, i-morphology kanye nokukhula kwepuleti esimweni sesinye esifanayo; futhi isiko lokunyakazisa esitholwe yi-bacterium siyindilinga, okungukuthi, i-mycelium ehlanganiswe yaba yiqoqo. Ngakho-ke, embonini yamagciwane enomphumela ofanayo wesiko lokudlidliza, isiko lokunyakazisa lisetshenziswe kabanzi. Indlela yokukhula ejikelezayo esikweni sezicubu nayo iwuhlobo lwesiko lokunyakazisa.

Indima yesiko elinyakazisayo:

1. ukudluliselwa kwesisindo, i-substrate noma i-metabolite idluliselwa kangcono futhi idlala indima ohlelweni.

2. i-oxygen encibilikisiwe, enkambisweni yokukhulisa i-aerobic, umoya uhlungwa uvulekile, ngakho-ke ngokusebenzisa ukuzulazula kungenza umoya omningi i-oxygen encibilikisiwe endaweni yokukhulisa imvelo.

3. ukufana kohlelo, okusiza ekuthathweni kwesampula nasekunqunyweni kwamapharamitha ahlukene.