Ukusetshenziswa kwe-Shaking Incubator ekukhuleni kwamaseli ebhayoloji

Isiko lezinto eziphilayo lihlukaniswe nge-static culture kanye ne-shaking culture. I-Shaking culture, eyaziwa nangokuthi i-suspension culture, iyindlela yokukhulisa lapho amaseli e-microbial afakwa khona emgodini oketshezi bese efakwa ku-shaker noma ku-oscillator ukuze kuqhume njalo. Isetshenziswa kabanzi ekuhlolweni kobunzima kanye ne-microbial expansion culture, futhi iyindlela yokukhulisa evame ukusetshenziswa ku-microbial physiology, i-biochemistry, ukubilisa kanye neminye imikhakha yocwaningo lwesayensi yokuphila. I-Shaking culture ayifaneleki ekukhuliseni izinto eziqukethe i-volatible chemical solvents, ukuhlushwa okuphansi kwamagesi aqhumayo kanye namagesi angashisi kakhulu kanye nezinto ezinobuthi.

Uyini umehluko phakathi kwamasiko aguquguqukayo nashukumayo?

I-CO2 incubator ilingisa indawo efanele yokukhulisa amaseli, okuhlanganisa izinga lokushisa, ukuhlushwa kwe-CO2 kanye nomswakama kanye nezinye izimo zangaphandle. Uma amaseli okuqala ekhuliswa ngaphansi kwezimo ezimile, amaseli anamathela odongeni olungezansi lweflaski bese kwakheka i-gradient yokuhlushwa kwe-oxygen encibilikisiwe kanye nezakhamzimba. Kodwa-ke, amaseli okumiswa ezimweni zokukhula okumile okunyakazisayo asusa i-gradient yokuhlushwa futhi andise ukuhlushwa kwe-oxygen encibilikisiwe, okuyinto elungele ukukhula. Ezindaweni zamagciwane kanye namaseli, isiko lokunyakazisa sithuthukisa ukuxhumana nezingxenye zemidiya kanye nokuhlinzekwa kwe-oxygen, ikakhulukazi kuma-fungus, ngaphandle kokwakheka kwe-hyphae noma amaqoqo. Ama-Mycobacteria atholakala esikweni esimile sesikhunta angabonakala ngokucacile yi-mycelium, i-morphology kanye nokukhula kwepuleti esimweni sesinye esifanayo; futhi isiko lokunyakazisa esitholwe yi-bacterium siyindilinga, okungukuthi, i-mycelium ehlanganiswe yaba yiqoqo. Ngakho-ke, embonini yamagciwane enomphumela ofanayo wesiko lokudlidliza, isiko lokunyakazisa lisetshenziswe kabanzi. Indlela yokukhula ejikelezayo esikweni sezicubu nayo iwuhlobo lwesiko lokunyakazisa.

Indima yesiko elinyakazisayo:

1. ukudluliselwa kwesisindo, i-substrate noma i-metabolite idluliselwa kangcono futhi idlala indima ohlelweni.

2. i-oxygen encibilikisiwe, enkambisweni yokukhulisa i-aerobic, umoya uhlungwa uvulekile, ngakho-ke ngokusebenzisa ukuzulazula kungenza umoya omningi i-oxygen encibilikisiwe endaweni yokukhulisa imvelo.

3. ukufana kohlelo, okusiza ekuthathweni kwesampula nasekunqunyweni kwamapharamitha ahlukene.