kuyini ukumiswa kwe-cell culture vs ukunamathela?
Amangqamuzana amaningi avela ezilwaneni ezinomgogodla, ngaphandle kwamangqamuzana e-hematopoietic namanye amangqamuzana ambalwa, ancike ekubambeleleni futhi kufanele akhuliswe endaweni engaphansi efanelekile eye yaphathwa ngokukhethekile ukuze ivumele ukunamathela kwamaseli nokusabalala. Kodwa-ke, amaseli amaningi nawo afanele isiko lokumiswa. Ngokufanayo, amangqamuzana ezinambuzane amaningi atholakalayo kwezentengiso akhula kahle kusiko lokunamathela noma lokumiswa.
Amaseli amisiwe angagcinwa kumaflask amasiko angazange alashwe isiko lezicubu, kodwa njengoba umthamo kanye nendawo yesiko ikhula, ukushintshaniswa kwegesi okwanele kuyavinjelwa futhi okuphakathi kudinga ukunyakaziswa. Lokhu kuyaluza kuvame ukufezwa nge-stirrer kazibuthe noma i-erlenmeyer flask ekunyakazeni incubator.
| Isiko Lokunamathela  | Ukumiswa Kwesiko  |
| Ifanele izinhlobo eziningi zamaseli, okuhlanganisa isiko lamaseli ayisisekelo | Okufanele amaseli kungaba yi-suspension cultured kanye namanye amaseli angabambeleli (isb, amaseli e-hematopoietic) |
| Idinga i-periodic subculture, kodwa ingabonwa kalula ngaphansi kwesibonakhulu esihlanekezelwe | Kulula ukwenza i-subculture, kodwa idinga ukubalwa kwamaseli nsuku zonke kanye nokuhlolwa kokusebenza ukuze kubonwe ukukhula; amasiko angahlanjululwa ukuze akhuthaze ukukhula |
| Amaseli akhiwe nge-enzymatic (isb. i-trypsin) noma ahlukanisiwe ngomshini | Akukho ukuhlukaniswa kwe-enzymatic noma komshini okudingekayo |
| Ukukhula kunqunyelwe indawo engaphezulu, okungase kukhawulele isivuno sokukhiqiza | Ukukhula kunqunyelwe ukuhlangana kwamaseli phakathi nendawo, ngakho-ke kungakhuliswa kalula |
| Imikhumbi ye-cellculture edinga ukwelashwa kwe-tissue culture surface | Ingagcinwa emikhunjini yesiko ngaphandle kokwelashwa kwezicubu zesiko, kodwa idinga ukuyaluza (okungukuthi, ukunyakaziswa noma ukunyakaziswa) ukuze kushintshwe igesi okwanele. |
| Isetshenziselwa i-cytology, ukuqoqwa kwamaseli okuqhubekayo kanye nezicelo eziningi zocwaningo | Isetshenziselwa ukukhiqizwa kwenqwaba yamaprotheni, ukuqoqwa kwe-batch cell kanye nezinhlelo zokusebenza eziningi zocwaningo |
| Thola i-incubator yakho ye-CO2 namapuleti amasiko amaseli manje:I-C180 140°C I-Incubator Yokushisa Okuphezulu ye-CO2Cell Culture Plate | Zitholele i-CO2 incubator shaker namaflaski e-erlenmeyer manje: |
Isikhathi sokuthumela: Jan-03-2024