Impembelelo yokutshintsha kobushushu kwinkcubeko yeseli kwi-CO2 incubator
Ubushushu buyiparameter ebalulekileyo kwinkcubeko yeseli kuba buchaphazela ukuzala kwakhona kweziphumo. Utshintsho lobushushu olungaphezulu okanye olungaphantsi kwama-37°C lunempembelelo ebaluleke kakhulu kwi-kinetics yokukhula kweseli yeeseli zezilwanyana ezincancisayo, olufana nolweeseli zebhaktheriya. Utshintsho kwimbonakalo yejini kunye notshintsho kwisakhiwo seseli, inkqubela yomjikelo weseli, ukuzinza kwe-mRNA kunokubonwa kwiiseli zezilwanyana ezincancisayo emva kweyure enye kwi-32ºC. Ukongeza ekuchaphazeleni ngokuthe ngqo ukukhula kweseli, utshintsho lobushushu lukwachaphazela i-pH yemidiya, njengoko ukunyibilika kwe-CO2 kutshintsha i-pH (i-pH iyanda kumaqondo obushushu aphantsi). Iiseli zezilwanyana ezincancisayo ezikhuliswe kakuhle zinokunyamezela ukwehla okukhulu kobushushu. Zingagcinwa kwi-4 °C kangangeentsuku ezininzi kwaye zinokunyamezela ukubanda ukuya kwi--196 °C (kusetyenziswa iimeko ezifanelekileyo). Nangona kunjalo, azinakunyamezela amaqondo obushushu angaphezulu kwe-2 °C ngaphezu kwesiqhelo ngaphezulu kweeyure ezimbalwa kwaye ziya kufa ngokukhawuleza kwi-40 °C nangaphezulu. Ukuqinisekisa ukuzala kwakhona okuphezulu kweziphumo, nokuba iiseli ziyasinda, kufuneka kuthathwe ingqalelo ukugcina ubushushu buhlala buhleli kangangoko kunokwenzeka ngexesha lokufakelwa nokuphathwa kweeseli ngaphandle kwe-incubator.
Izizathu zokutshintshatshintsha kobushushu ngaphakathi kwi-incubator
Uya kube uqaphele ukuba xa ucango lwe-incubator luvulwa, ubushushu buyehla ngokukhawuleza bufikelele kwixabiso elimiselweyo le-37 °C. Ngokubanzi, ubushushu buya kubuyela kwimeko yesiqhelo kwimizuzu embalwa emva kokuba ucango luvaliwe. Enyanisweni, ii-static cultures zifuna ixesha lokubuyela kwimeko yesiqhelo kwi-incubator. Izinto ezininzi zinokuchaphazela ixesha elithathwayo ukuze i-cell culture ibuyele kwimeko yesiqhelo emva konyango ngaphandle kwe-incubator. Ezi zinto ziquka:
- ▶ubude bexesha iiseli ziphumile kwi-incubator
- ▶uhlobo lweflaski apho iiseli zikhulela khona (ijiyometri ichaphazela ukudluliselwa kobushushu)
- ▶Inani lezikhongozeli ezikwi-incubator.
- ▶Ukudibana ngqo kweeflaski neshelufu yentsimbi kuchaphazela ukutshintshiselana kobushushu kunye nesantya sokufikelela kubushushu obufanelekileyo, ngoko ke kungcono ukuphepha ukuqokelelana kweeflaski uze ubeke isitya ngasinye
- ▶ ngqo kwishelufu ye-incubator.
Ubushushu bokuqala bazo naziphi na izikhongozeli ezintsha kunye nemidiya esetyenzisiweyo iya kuchaphazela ixesha elithathwayo ukuze iiseli zibe kumgangatho wazo ofanelekileyo; okukhona ubushushu bazo buphantsi, kokukhona kuthatha ixesha elide.
Ukuba zonke ezi zinto ziyatshintsha ngokuhamba kwexesha, ziya kwandisa umahluko phakathi kwezilingo. Kubalulekile ukunciphisa oku kuguquguquka kobushushu, nokuba akunakwenzeka ukulawula yonke into (ingakumbi ukuba abantu abaninzi basebenzisa into efanayo).Isifukamisi se-CO2).
Indlela yokunciphisa ukutshintsha kwamaqondo obushushu kunye nokunciphisa ixesha lokubuyisela amaqondo obushushu
Ngokufudumeza i-medium kwangaphambili
Abanye abaphandi baqhele ukufudumeza iibhotile ezipheleleyo ze-media kwi-37°C bath ukuze bazifikise kolu bushushu ngaphambi kokuba basebenzise. Kukwanokwenzeka ukuba ufudumeze i-medium kwi-incubator esetyenziselwa kuphela ukufudumeza okuphakathi kwaye kungekhona ukukhuliswa kweeseli, apho i-medium inokufikelela kubushushu obufanelekileyo ngaphandle kokuphazamisa ukukhuliswa kweeseli kwenye i-incubator. Kodwa oku, ngokokwazi kwethu, kudla ngokuba yindleko efikelelekayo.
Ngaphakathi kwi-Incubator
Vula ucango lwe-incubator kancinci kangangoko unako kwaye uluvale ngokukhawuleza. Ziphephe iindawo ezibandayo, ezidala umahluko kubushushu kwi-incubator. Shiya isithuba phakathi kweeflaski ukuze umoya ujikeleze. Iishelufu ezingaphakathi kwi-incubator zinokugqobhozwa. Oku kuvumela ukusasazwa kobushushu okungcono njengoko kuvumela umoya ukuba udlule kwimingxunya. Nangona kunjalo, ubukho bemingxunya bunokukhokelela kumahluko ekukhuleni kweseli, kuba kukho umahluko kubushushu phakathi kwendawo enemingxunya kunye nendawo ene-meta. Ngenxa yezi zizathu, ukuba uvavanyo lwakho lufuna ukukhula okufanayo kwenkcubeko yeseli, ungabeka iiflaski zenkcubeko kwizixhobo zesinyithi ezineendawo ezincinci zokunxibelelana, ezidla ngokungadingeki kwinkcubeko yeseli eqhelekileyo.
Ukunciphisa Ixesha Lokucubungula Iiseli
Ukunciphisa ixesha elichithwa kwinkqubo yonyango lweeseli, kufuneka
- ▶ Lungiselela zonke izixhobo ezifunekayo ngaphambi kokuba uqale ukusebenza.
- ▶Sebenza ngokukhawuleza nangokulula, uphonononge iindlela zovavanyo kwangaphambili ukuze imisebenzi yakho iphindaphindeke kwaye izenzekele.
- ▶Nciphisa ukudibana kolwelo nomoya ojikeleze umhlaba.
- ▶ Gcina ubushushu obungaguqukiyo kwilebhu yokukhulisa iiseli apho usebenza khona.
Ixesha leposi: Jan-03-2024