Ingakanani i-medium ekufuneka yongezwe kwiflaski kwi-incubator eshukumayo?
Iiflaski ze-Erlenmeyer zeRADOBIO ezingengomhlaza zenzelwe ngokukodwa ukukhulisa iiseli ezixhonyiweyo kuquka iiseli ze-HEK 293 kunye ne-CHO kwi-ai-incubator eshukumisayoEzi flasks zinesiseko esithe tyaba kunye nesigqubuthelo esinomoya, nto leyo ezenza zifaneleke kuphuhliso lwenkqubo encinci, ukukhulisa, kunye nokulima ngamanqanaba amaninzi. Isigqubuthelo esinomoya sidibanisa i-0.2 μm hydrophobic membrane, evumela ukutshintshiselana kwegesi ngokufanelekileyo ngelixa ithintela ungcoliseko lweentsholongwane kunye nokungena kwamanzi, ukuqinisekisa iimeko ezifanelekileyo zokukhula kweeseli.
Iinkcukacha eziphambili kunye nezikhokelo zokusetyenziswa
- 1.Ukuphucula iVolumu yokusebenza.
- •Umthamo ophakathi kufuneka ugcinwe kwi-20–30% yomthamo weflask iyonke ukuqinisekisa ukudluliselwa kweoksijini okwaneleyo kunye nokunciphisa uxinzelelo lokucheba
- • Amanqaku okugqiba acacileyo kwiflaski avumela ukulinganiswa komthamo ngokuchanekileyo .
- •Umthamo ophakathi kufuneka ugcinwe kwi-20–30% yomthamo weflask iyonke ukuqinisekisa ukudluliselwa kweoksijini okwaneleyo kunye nokunciphisa uxinzelelo lokucheba
- 2. Iiparameter zokushukumisa.
- •Icetyisiweisihluzi se-incubatorisantya sokungcangcazela: 75–120 RPM (ingahlengahlengiswa ngokusekelwe kwiimfuno zovavanyo)
- •Kwiiflaski ezinkulu (≥3L), nciphisa isantya siye kwi-90 RPM ukuze uthintele ukucheba ulwelo olugqithisileyo kunye nokugqwala kwegwebu
- •Icetyisiweisihluzi se-incubatorisantya sokungcangcazela: 75–120 RPM (ingahlengahlengiswa ngokusekelwe kwiimfuno zovavanyo)
Iiparameter ezicetyiswayo zeeseli ze-CHO kunye ne-HEK 293
| Umqulu weFlaski | Umthamo wokusebenza | Isantya Sokushukuma |
|---|---|---|
| 125 ml | 25 ml | 120 RPM |
| 250 ml | 60 mL | 120 RPM |
| 500 ml | 150 ml | 120 RPM |
| 1000 mL | 350 ml | 120 RPM |
| 1500 mL | 400 mL | 120 RPM |
| 2000 mL | 400 mL | 120 RPM |
| 3000 mL | 800 mL | 90 RPM |
| 5000 mL | 2500 mL | 90 RPM |
Qaphela: Idatha isebenza kwi-CHO Stable, CHO Transient, kunye ne-HEK 293 Transient cell lines
Iinkcukacha zobuchwephesha ezongezelelweyo
- 1. Izinto kunye nokungabi nazintsholongwane.
- •Ukhetho lwezinto zePETG/PC:I-PETG ibonelela ngokubonakala okuphezulu kunye nokumelana nempembelelo; I-PC ibonelela ngozinzo oluphezulu lobushushu (imelana ne-autoclaving kwi-121°C)
-
- •Ukubulala iintsholongwane: Ifakwe i-gamma-ray, ipakishwe nganye nganye, iqinisekisiwe ukuba ayinayo i-DNase/RNase, ayinayo i-endotoxin, kwaye ihambelana nemigangatho ye-USP Class VI
- •Ukhetho lwezinto zePETG/PC:I-PETG ibonelela ngokubonakala okuphezulu kunye nokumelana nempembelelo; I-PC ibonelela ngozinzo oluphezulu lobushushu (imelana ne-autoclaving kwi-121°C)
- 2. Iingenelo zoYilo.
- •Isivalo se-Ergonomic: Inceda ekuphatheni ngokukhuselekileyo nasekuhambelaneni nee-pipettes/ii-cell scrapers
- •Iimodeli Ezididekileyo Ezifumanekayo: Ziphucula ukusebenza kakuhle kokuxuba iinkcubeko ezinoxinano olukhulu ngokwandisa ukudluliselwa kweoksijini (kLa)
- •Isivalo se-Ergonomic: Inceda ekuphatheni ngokukhuselekileyo nasekuhambelaneni nee-pipettes/ii-cell scrapers
- 3. Amanqaku okusetyenziswa okubalulekileyo.
- •Kuphephe Ukuzalisa Ngokugqithisileyo: Ubuninzi obungaphezulu kwama-30% bunokunciphisa ukutshintshiselana kwegesi, nto leyo ekhokelela kwi-hypoxia kwiinkcubeko ze-aerobic
- •Jonga i-pH/Oksijini: Gcina i-pH kwi-7.0–7.4 (iiseli ze-CHO) kunye neoksijini enyibilikisiweyo (DO) >40% ukuze kubekho imetabolism efanelekileyo
- •Ubukhali bokucheba: Kwiiseli ezibuthathaka ekuchebeni (umz., iiseli ze-T eziphambili), sebenzisa isantya esiphantsi (80–100 RPM) okanye iiarhente ezichasene negwebu
- •Kuphephe Ukuzalisa Ngokugqithisileyo: Ubuninzi obungaphezulu kwama-30% bunokunciphisa ukutshintshiselana kwegesi, nto leyo ekhokelela kwi-hypoxia kwiinkcubeko ze-aerobic
Izicelo ezingaphaya kokulima okuqhelekileyo
- •Iinkqubo zokuPhefumla: Hlanganisa neendlela zokutya ukuze ufumane izondlo eziqhubekayo, ufezekise ubuninzi beeseli >20 × 10⁶ iiseli/mL
- •Iinkcubeko zeMicrobial: Zilungele ukubiliswa kwebhaktiriya/imvubelo kunye noyilo oludidayo lokuphucula umoya
- •IiProtocol zeScale-Up: Xa utshintsha ukusuka kwiiflasks ukuya kwi-bioreactors, gcina izinga lokudlulisa ioksijini yevolumu (kLa) lihlala lihleli ngokulungelelanisa ukushukuma kunye nomoya.
Ixesha leposi: Agasti-17-2025