Ungakhetha kanjani i-amplitude ye-Shaker efanele?
Iyini i-amplitude ye-shaker?
I-amplitude ye-shaker ububanzi bephalethi ekuhambeni okuyindilinga, ngezinye izikhathi okubizwa ngokuthi "i-oscillation diameter" noma "i-track diameter": Ø. I-Radobio inikeza ama-shaker ajwayelekile anama-amplitudes angu-3mm, 25mm, 26mm no-50mm,. Amashaker enziwe ngokwezifiso anamanye amasayizi we-amplitude ayatholakala.
Liyini Isilinganiso Sokudlulisa I-Oxygen (OTR)?
I-Oxygen Transfer Rate (OTR) ukusebenza kahle komoya-mpilo udluliswa usuka emkhathini uye oketshezini. Ukuphakama kwevelu ye-OTR kusho ukuphakama kokudluliswa kwe-oxygen kahle.
Umthelela we-amplitude kanye nesivinini sokuzungeza
Zombili lezi zici zithinta ukuxutshwa kwento ephakathi eflaskini yesiko. Ukuxuba okungcono kakhulu, kuba ngcono izinga lokudlulisa umoya-mpilo (OTR). Ukulandela lezi ziqondiso, i-amplitude efanelekile kakhulu nesivinini sokujikeleza singakhethwa.
Ngokuvamile, ukukhetha i-amplitude engu-25mm noma 26mm kungasetshenziswa njenge-amplitude yendawo yonke yazo zonke izinhlelo zokusebenza zesiko.
Amagciwane, imvubelo kanye namasiko fungal:
Ukudluliswa komoyampilo kumaflask okunyakazisa akusebenza kahle kakhulu kunakuma-bioreactors. Ukudluliswa komoyampilo kungase kube isici esikhawulelayo kumasiko e-shake flask ezimweni eziningi. I-amplitude ihlobene nobukhulu be-conical flasks: ama-flasks amakhulu asebenzisa ama-amplitudes amakhulu.
Isincomo: i-amplitude engu-25mm yamaflasks ayi-conical kusuka ku-25ml kuya ku-2000ml.
50 mm amplitude for flasks conical kusuka 2000 ml kuya 5000 ml.
I-Cell Culture:
* Isiko lamaseli ezilwane ezincelisayo anesidingo somoya-mpilo esiphansi uma kuqhathaniswa.
* Kumaflask e-shaker angu-250mL, ukulethwa komoyampilo owanele kungahlinzekwa ngebanga elibanzi kakhulu lama-amplitude nesivinini (20-50mm amplitude; 100-300rpm).
* Kumaflasks amakhulu (amaflaski eFernbach) kunconywa i-amplitude engu-50mm.
* Uma kusetshenziswa izikhwama zamasiko alahlwayo, kunconywa i-50mm amplitude.
I-Microtiter kanye namapuleti ajulile:
Kumapuleti ama-microtiter namapuleti ajulile kunezindlela ezimbili ezihlukene zokuthola ukudluliswa komoya-mpilo okuphezulu!
* 50 mm amplitude ngesivinini esingekho ngaphansi kuka-250 rpm.
* Sebenzisa i-3mm amplitude ku-800-1000rpm.
Ezimweni eziningi, ngisho noma i-amplitude enengqondo ikhethiwe, ingase ingakhulisi ivolumu ye-bioculture, ngoba ukwanda kwevolumu kungathonywa yizici eziningana. Isibonelo, uma isici esisodwa noma ezimbili kweziyishumi zingalungile, khona-ke ukwanda kwevolumu yesiko kuzokhawulwa kungakhathaliseki ukuthi ezinye izici zinhle kangakanani, noma kungase kuthiwe ukukhetha okufanele kwe-amplitude kuzoholela ekwenyukeni okuphawulekayo kwe-incubator uma okuwukuphela kwesici esinqunyelwe kumthamo wesiko ukulethwa komoyampilo. Isibonelo, uma umthombo wekhabhoni uyisici esikhawulelayo, kungakhathaliseki ukuthi kuhle kangakanani ukudluliswa komoya-mpilo, ivolumu yesiko elifiswayo ngeke lizuzwe.
I-amplitude kanye nesivinini sokuzungeza
Kokubili i-amplitude nesivinini sokujikeleza kungaba nomthelela ekudlulisweni komoyampilo. Uma amasiko amaseli etshalwa ngesivinini esiphansi kakhulu sokujikeleza (isb., 100 rpm), umehluko we-amplitude unomthelela omncane noma ongabonakali ekudluliselweni komoyampilo. Ukuze kuzuzwe ukudluliswa kwe-oxygen ephakeme kakhulu, isinyathelo sokuqala ukwandisa isivinini sokujikeleza ngangokunokwenzeka, futhi ithreyi izoba nokulinganisela kahle ngesivinini. Akuwona wonke amaseli angakhula kahle ngama-oscillation esivinini esikhulu, futhi amanye amaseli azwela amandla okugunda angase afe ngenxa yesivinini esikhulu sokujikeleza.
Amanye amathonya
Ezinye izici zingaba nomthelela ekudlulisweni komoyampilo:.
* Ivolumu yokugcwalisa, ama-flasks ayi-conical kufanele agcwaliswe angabi ngaphezu kwengxenye eyodwa kwezintathu yevolumu ephelele. Uma ukudluliswa kwe-oxygen okuphezulu kuzofezwa, gcwalisa ungabi ngaphezu kuka-10%. Ungalokothi ugcwalise ku-50%.
* Ama-Spoilers: Ama-spoilers ayasebenza ekuthuthukiseni ukudluliswa komoya-mpilo kuzo zonke izinhlobo zamasiko. Abanye abakhiqizi batusa ukusetshenziswa kwamaflask "I-Ultra High Yield". Ama-spoiler kulawa maflask akhuphula ukungqubuzana koketshezi futhi isinyakazi singase singafinyeleli isivinini esibekiwe.
Ukuhlobana phakathi kwe-amplitude nesivinini
Amandla we-centrifugal ku-shaker angabalwa kusetshenziswa i-equation elandelayo
FC = rpm2× ubukhulu
Kukhona ubudlelwano bomugqa phakathi kwamandla we-centrifugal kanye ne-amplitude: uma usebenzisa i-amplitude engu-25 mm ukuya ku-50 mm amplitude (ngesivinini esifanayo), amandla e-centrifugal akhuphuka nge-factor of 2.
Kukhona ubudlelwano obuyisikwele phakathi kwamandla we-centrifugal kanye nesivinini sokujikeleza.
Uma ijubane likhushulwa nge-2 (amplitude efanayo), amandla e-centrifugal akhuphuka nge-4. Uma ijubane lenyuka nge-3 factor, amandla e-centrifugal akhuphuka nge-9 factor!
Uma usebenzisa i-amplitude engu-25 mm, fukamela ngesivinini esinikeziwe. Uma ufisa ukuzuza amandla afanayo e-centrifugal nge-amplitude engu-50 mm, isivinini sokujikeleza kufanele sibalwe njengempande yesikwele engu-1/2, ngakho-ke kufanele usebenzise u-70% wejubane lokujikeleza ukuze ufinyelele izimo ezifanayo zokufukamela.
Sicela uqaphele ukuthi lokhu okungenhla kuyindlela yasetiyetha kuphela yokubala amandla e-centrifugal. Kukhona ezinye izici ezinomthelela ezinhlelweni zangempela. Le ndlela yokubala inikeza amanani acishe abe yizinjongo zokusebenza.
Isikhathi sokuthumela: Jan-03-2024