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Ungayikhetha kanjani i-amplitude ye-shaker efanele?
Kuyini ukuphinhlaka komjondolo?
I-amplitude ye-shaker ububanzi be-pallet ekunyakazeni okujikelezayo, kwesinye isikhathi ibizwa nge- "Oscillation Ububanzi" noma "Ithrekhi Diameter" uphawu: Ø. I-Radobio inikeza ama-Shaker ajwayelekile nge-Amplitudes of 3mm, 25mm, 26mm no-50mm,. AbakwaShaker abenziwe ngokwezifiso nabanye amasayizi amplitude ayatholakala.
Yini isilinganiso se-Oxygen Transfer (OTR)?
Izinga lokudlulisa le-oksijini (i-OTR) lisebenza kahle komoya-mpilo lidluliselwa emkhathini liye koketshezi. Inani eliphakeme le-OTR lisho ukusebenza kahle kokudlulisa umoya-mpilo.
Umphumela wokuphakama nokujikeleza isivinini
Zombili lezi zinto zithinta ukuxubana okuphakathi kwiflask yesiko. Okungcono ukuxuba, okungcono isilinganiso sokudlulisa umoya-mpilo (OTR). Ukulandela le mihlahlandlela, ijubane elifanele kakhulu nokujikeleza lingakhethwa.
Ngokuvamile, ukukhetha ama-amplitude we-25mm noma angama-26mm angasetshenziswa njenge-amplitude yendawo yonke yazo zonke izinhlelo zokusebenza zesiko.
Amagciwane, imvubelo kanye namasiko fungal:
Ukudluliswa kwe-oxygen kuma-flasks ashukumisayo kusebenza kahle kakhulu kunaseBoondeactors. Ukudluliswa kwe-oksijini kungaba yinto ekhawula yomakinasi amasiko amaningi ezimweni eziningi. I-amplitude ihlobene nosayizi we-conical Flasks: Ama-Flasks amakhulu asebenzisa ama-amplitude amakhulu.
Isincomo: I-25mm Amplitude yama-conical flasks asuka ku-25ml aya ku-2000ml.
I-50 mm amplitude ye-conical flasks kusuka ku-2000 ml kuya ku-5000 ml.
Isiko Leselula:
* Isiko laseMammalian Cell linesidingo esiphansi se-oxygen.
* Okwe-250ml Shaker Flasks, ukulethwa ngokwanele komoya-mpilo kunganikezwa ngohla olubanzi lwama-amplitudes kanye nejubane (20-50mm amplitude; 100-300rpm).
* Ukuze uthole ama-Flasks akhudlwana (Fernbach Flasks) Ukuphakanyiswa kwe-50mm kunconyiwe.
* Uma kusetshenziswa izikhwama zesiko ezilahliwe, kunconywa ama-50mm amplitude.
I-MicroTiter kanye namapuleti ajulile:
Nge-microtiter kanye namapuleti ajulile kunezindlela ezimbili ezihlukile zokuthola ukudluliswa kwe-oxygen ephezulu!
* 50 mm amplitude ngejubane elingaphansi kwama-250 rpm.
* Sebenzisa i-3mm amplitude ku-800-1000rpm.
Ezimweni eziningi, noma ngabe kukhethwe inani elifanelekile, kungenzeka kungandise ivolumu ye-biociture, ngoba ukwanda kwevolumu kungathonywa yizici eziningana. Isibonelo, uma isici esisodwa noma ezimbili kweziyishumi zingezinhle, khona-ke ukwanda kwevolumu yesiko kuzonqunyelwa noma ngabe kukuhle kangakanani ezinye izinto, noma kungavela ukuthi okungukuphela kokuphakanyiswa kwemali kuzoholela ekunikezelweni kwevolumu yesiko. Isibonelo, uma umthombo wekhabhoni uyinto ekhawulelwe, noma ngabe ukudluliswa komoya-mpilo kuyinto enhle kangakanani, ivolumu yesiko efiselekayo ngeke litholakale.
Ijubane le-Amplitude ne-Rotation
Zombili ijubane lokukhulisa nokujikeleza kungaba nomthelela ekudluliseleni i-oxygen. Uma amasiko weseli atshalwa ngejubane eliphansi kakhulu elijikelezayo (isib. Ukufinyelela ukudluliswa okuphezulu kakhulu komoya-mpilo, isinyathelo sokuqala ukukhulisa ijubane lokujikeleza ngangokunokwenzeka, futhi ugqoko luzolingana kahle ngejubane. Akuwona wonke amaseli angakhula kahle ngama-oscillations aphezulu we-oscillations, namanye amaseli azwela amabutho ama-shear angafa ngesivinini esikhulu sokushintshana.
Amanye amathonya
Ezinye izinto zingaba nomthelela ekudluliseleni i-oxygen :.
* Ukugcwalisa ivolumu, amaflathi ahlanganisiwe kufanele agcwaliswe ngaphandle kwengxenye eyodwa kwezintathu yevolumu ephelele. Uma ukuhanjiswa okuphezulu komoya-mpilo kuzotholakala, ugcwalise kungabi ngaphezu kwe-10%. Ungalokothi ugcwalise ama-50%.
* Abaphangi: Abaphangi bayasebenza ekuthuthukiseni ukudluliswa kwe-oxygen kuzo zonke izinhlobo zamasiko. Abanye abakhiqizi batusa ukusetshenziswa kwe- "Ultra High Reage" Flasks ". Abaphangi kulezi zimbozo bakhuphuka ukungezwani okuwuketshezi futhi i-shaker ingahle ingafinyeleli isivinini esisethiwe esisethiwe.
Ukuhlangana phakathi kokuphakama kanye nesivinini
Amandla we-centrifugal ku-shaker angabalwa kusetshenziswa i-equation elandelayo
FC = RPM2× Amplitude
Kunobudlelwano obuqondile phakathi kwamandla we-centrifugal kanye ne-amplitude: Uma usebenzisa ama-amplitude angama-25 mm kuya ku-50 mm amplitude (ngejubane elifanayo), amandla we-centrifugal akhuphuka ngesici esingu-2.
Ubudlelwano besikwele bukhona phakathi kweCentrifugal Force nejubane lokujikeleza.
Uma ijubane lenyuswe ngesici esingu-2 (amplifututude efanayo), amandla we-centrifugal akhuphuka ngesici esingu-4. Uma ijubane likhuphuke ngesici se-3, amandla e-centrifugal akhuphuka ngesici esingu-9!
Uma usebenzisa i-amplitude yama-25 mm, afaka ngejubane elinikeziwe. Uma ufisa ukufeza amandla afanayo e-centrifugal nge-amplitude angu-50 mm, ijubane lokushintshana kufanele libalwe njengempande yesikwele engu-1/2, ngakho-ke kufanele usebenzise ama-70% wejubane lokujikeleza ukuze uzuze izimo ezifanayo zokujikeleza ukuze uzuze izimo ezifanayo zokujikeleza ukuze uzuze izimo ezifanayo zokujikeleza ukuze uzuze izimo ezifanayo zokujikeleza ukuze uzuze izimo ezifanayo zokujikeleza.
Uyacelwa ukuthi uqaphele ukuthi okungenhla kuyindlela yethiyori yokubala ama-centrifugal Force. Kukhona ezinye izinto ezithonya kuzicelo zangempela. Le ndlela yokubala inika amanani alinganiselwe ngezinjongo zokusebenza.
Isikhathi sePosi: Jan-03-2024