Ingakanani i-medium okufanele ifakwe ebhodleleni ku-incubator enyakazayo?
Ama-flask e-Erlenmeyer e-RADOBIO ayisitho samangqamuzana ahlanzekile aklanyelwe ngqo ukukhulisa amaseli okumiswa okuhlanganisa amaseli e-HEK 293 kanye ne-CHO ku-i-incubator ethuthumelayo. Lawa maflaski anesisekelo esiyisicaba kanye nesivalo esinomoya, okwenza afanelekele ukuthuthukiswa kwenqubo encane, ukukhulisa, kanye nokutshalwa kwezigaba eziningi. Isivalo esinomoya sihlanganisa ulwelwesi olungenamanzi olwe-0.2 μm, oluvumela ukushintshaniswa kwegesi okuphumelelayo ngenkathi kuvimbela ukungcola kwamagciwane kanye nokungena kwamanzi, okuqinisekisa izimo zokukhula kwamaseli ezifanele kakhulu.
Imininingwane Eyinhloko kanye Neziqondiso Zokusebenzisa
- 1.Ukuthuthukisa Ivolumu Yokusebenza.
- •Umthamo ophakathi kufanele ugcinwe ku-20-30% wesamba samandla ebhodle ukuze kuqinisekiswe ukudluliswa okwanele komoya-mpilo nokunciphisa ukucindezeleka kokucheba.
- • Amamaki okuqeda acacile ebhodleleni avumela ukulinganiswa okunembile kwevolumu .
- •Umthamo ophakathi kufanele ugcinwe ku-20-30% wesamba samandla ebhodle ukuze kuqinisekiswe ukudluliswa okwanele komoya-mpilo nokunciphisa ukucindezeleka kokucheba.
- 2.Amapharamitha Okunyakazisa.
- •Kunconyiweumshini wokufukula i-incubatorisivinini sokuthuthumela: 75–120 RPM (kungalungiswa ngokusekelwe ezidingweni zokuhlola)
- •Kumaflaski amakhulu (≥3L), yehlisa isivinini sibe yi-90 RPM ukuze uvimbele ukucheba ngokweqile koketshezi kanye nokugqwala
- •Kunconyiweumshini wokufukula i-incubatorisivinini sokuthuthumela: 75–120 RPM (kungalungiswa ngokusekelwe ezidingweni zokuhlola)
Amapharamitha Anconyiwe Amaseli e-CHO kanye ne-HEK 293
| Ivolumu Yesitsha | Ivolumu Yokusebenza | Isivinini Sokushaqeka |
|---|---|---|
| 125 ml | 25 ml | 120 RPM |
| 250 ml | 60 ml | 120 RPM |
| 500 ml | 150 ml | 120 RPM |
| 1000 mL | 350 ml | 120 RPM |
| 1500 mL | 400 ml | 120 RPM |
| 2000 mL | 400 ml | 120 RPM |
| 3000 mL | 800 ml | 90 RPM |
| 5000 mL | 2500 ml | 90 RPM |
Qaphela: Idatha isebenza emigqeni yamaseli e-CHO Stable, CHO Transient, kanye ne-HEK 293 Transient
Imininingwane Yobuchwepheshe Eyengeziwe
- 1. Izinto kanye Nokungazali.
- •Izinketho Zezinto Ze-PETG/ze-PC:I-PETG inikeza ukucaca okuphezulu kanye nokumelana nomthelela; i-PC inikeza ukuzinza okuphezulu kokushisa (imelana ne-autoclaving ku-121°C)
-
- •Ukuhlanza: Kufakwe imisebe nge-gamma, kupakishwe ngakunye, akuna-DNase/RNase eqinisekisiwe, akuna-endotoxin, futhi kuhambisana nezindinganiso ze-USP Class VI
- •Izinketho Zezinto Ze-PETG/ze-PC:I-PETG inikeza ukucaca okuphezulu kanye nokumelana nomthelela; i-PC inikeza ukuzinza okuphezulu kokushisa (imelana ne-autoclaving ku-121°C)
- 2. Izinzuzo Zokuklama.
- •I-Ergonomic Cap: Isiza ekuphatheni okuphephile kanye nokuhambisana nama-pipettes/ama-cell scraper
- •Amamodeli Adidekile Atholakalayo: Kuthuthukisa ukusebenza kahle kokuxubana kwezitshalo ezinobuningi obukhulu ngokwandisa ukudluliswa komoya-mpilo (kLa)
- •I-Ergonomic Cap: Isiza ekuphatheni okuphephile kanye nokuhambisana nama-pipettes/ama-cell scraper
- 3. Amanothi Okusetshenziswa Okubalulekile.
- •Gwema Ukugcwalisa Kakhulu: Ubuningi obungaphezu kuka-30% bunganciphisa ukushintshana kwegesi, okuholela ku-hypoxia ekukhuleni kwe-aerobic
- •Qapha i-pH/i-Oxygen: Gcina i-pH ku-7.0–7.4 (amaseli e-CHO) kanye ne-oxygen encibilikisiwe (DO) >40% ukuze kusetshenziswe kahle i-metabolism
- •Ukuzwela Kokugunda: Kumaseli azwela ukugunda (isb., amaseli e-T ayinhloko), sebenzisa isivinini esiphansi (80–100 RPM) noma ama-anti-foam agents
- •Gwema Ukugcwalisa Kakhulu: Ubuningi obungaphezu kuka-30% bunganciphisa ukushintshana kwegesi, okuholela ku-hypoxia ekukhuleni kwe-aerobic
Izicelo Ezingaphezu Kokulima Okujwayelekile
- •Izinhlelo Zokugeleza: Hlanganisa nemigqa yokuphakelayo ukuze uthole ukuphakelwa okuqhubekayo kwezakhamzimba, ufinyelele ubuningi bamaseli >20 × 10⁶ amaseli/mL
- •Izitshalo Ezincane: Zifanelekela ukubiliswa kwamagciwane/imvubelo ngemiklamo edidayo yokuthuthukisa umoya
- •Amaphrothokholi Okukhulisa Usayizi: Uma ushintsha kusuka kumaflasks uye kuma-bioreactors, gcina izinga lokudlulisa umoya-mpilo elihambisanayo (kLa) ngokulungisa ukushukuma nomoya
Isikhathi sokuthunyelwe: Agasti-17-2025