Umphumela wokwehluka kwezinga lokushisa ekukhuleni kwamaseli ku-CO2 incubator
Izinga lokushisa liyipharamitha ebalulekile ekukhuleni kwamaseli ngoba lithinta ukuphindaphindwa kwemiphumela. Izinguquko zokushisa ezingaphezu noma ngaphansi kuka-37°C zinomthelela obalulekile kakhulu ekukhuleni kwamaseli kwamaseli ezilwane ezincelisayo, okufana nokwamangqamuzana ebhaktheriya. Izinguquko ekubonakalisweni kwezakhi zofuzo kanye nokuguqulwa kwesakhiwo samaseli, ukuqhubeka komjikelezo weseli, ukuzinza kwe-mRNA kungatholakala kumaseli ezilwane ezincelisayo ngemva kwehora elilodwa ku-32ºC. Ngaphezu kokuthinta ngqo ukukhula kwamaseli, izinguquko zokushisa zithinta ne-pH yemidiya, njengoba ukuncibilika kwe-CO2 kushintsha i-pH (i-pH iyanda emazingeni okushisa aphansi). Amaseli ezilwane ezincelisayo akhuliswe angabekezelela ukwehla okukhulu kwezinga lokushisa. Angagcinwa ku-4 °C izinsuku eziningana futhi angabekezelela ukubanda ku--196 °C (kusetshenziswa izimo ezifanele). Kodwa-ke, awakwazi ukubekezelela amazinga okushisa angaphezu kuka-2 °C ngaphezu kokujwayelekile isikhathi esingaphezu kwamahora ambalwa futhi azofa ngokushesha ku-40 °C nangaphezulu. Ukuqinisekisa ukuphindaphindwa okuphezulu kwemiphumela, noma ngabe amaseli asinda, kudingeka kuthathwe ukunakekelwa ukuze kugcinwe izinga lokushisa lingaguquki ngangokunokwenzeka ngesikhathi sokufukamela nokuphathwa kwamaseli ngaphandle kwe-incubator.
Izizathu zokushintshashintsha kwezinga lokushisa ngaphakathi kwe-incubator
Uzobe uqaphele ukuthi uma umnyango we-incubator uvulwa, izinga lokushisa lehla ngokushesha liye ku-37 °C. Ngokuvamile, izinga lokushisa lizobuya lingene ngemizuzu embalwa ngemva kokuvalwa komnyango. Eqinisweni, ama-static cultures adinga isikhathi sokubuya lifike ezingeni lokushisa elibekiwe ku-incubator. Izinto eziningana zingathinta isikhathi esithathwayo ukuze i-cell culture ibuyele ezingeni lokushisa ngemva kokwelashwa ngaphandle kwe-incubator. Lezi zinto zifaka:
- ▶ubude besikhathi amaseli aphumile ku-incubator
- ▶uhlobo lweflaski lapho kukhuliswa khona amaseli (i-geometry ithinta ukudluliselwa kokushisa)
- ▶Inani leziqukathi eziku-incubator.
- ▶Ukuthintana ngqo kwamaflaski neshelufu lensimbi kuthinta ukushintshana kokushisa kanye nesivinini sokufinyelela izinga lokushisa elifanele, ngakho-ke kungcono ukugwema izinqwaba zamaflaski nokubeka isitsha ngasinye
- ▶ ngqo eshalofini le-incubator.
Izinga lokushisa lokuqala lanoma yiziphi izitsha ezintsha kanye nemidiya esetshenziswayo kuzothinta nesikhathi esithathwayo ukuze amaseli abe sesimweni sawo esifanele; uma izinga lokushisa lawo liphansi, kuthatha isikhathi eside.
Uma zonke lezi zici zishintsha ngokuhamba kwesikhathi, zizokwandisa nokwehluka phakathi kokuhlolwa. Kuyadingeka ukunciphisa lokhu kushintshashintsha kwezinga lokushisa, noma ngabe akunakwenzeka njalo ukulawula yonke into (ikakhulukazi uma abantu abaningana besebenzisa okufanayo).Isithwali se-CO2).
Indlela yokunciphisa ukushintshashintsha kwezinga lokushisa nokunciphisa isikhathi sokubuyisa izinga lokushisa
Ngokufudumeza i-medium kusengaphambili
Abanye abacwaningi bajwayele ukufudumeza amabhodlela aphelele e-media kubhavu wamanzi angu-37°C ukuze bawalethe kuleli zinga lokushisa ngaphambi kokusebenzisa. Kungenzeka futhi ukufudumeza i-medium ku-incubator esetshenziselwa kuphela ukufudumeza okuphakathi nendawo hhayi ukukhuliswa kwamaseli, lapho i-medium ingafinyelela izinga lokushisa elifanele ngaphandle kokuphazamisa ukukhuliswa kwamaseli kwenye i-incubator. Kodwa lokhu, ngokwazi kwethu, ngokuvamile akuyona imali engabizi.
Ngaphakathi kwe-Incubator
Vula umnyango we-incubator kancane ngangokunokwenzeka bese uwuvala ngokushesha. Gwema izindawo ezibandayo, ezidala umehluko wokushisa ku-incubator. Shiya isikhala phakathi kwamaflaski ukuze uvumele umoya ujikeleze. Amashalofu angaphakathi kwe-incubator angabhoboka. Lokhu kuvumela ukusatshalaliswa kokushisa okungcono njengoba kuvumela umoya ukuthi udlule emigodini. Kodwa-ke, ukuba khona kwemigodi kungaholela ekwehlukeni ekukhuleni kwamaseli, ngoba kukhona umehluko wokushisa phakathi kwendawo enemigodi nendawo ene-meta. Ngenxa yalezi zizathu, uma izivivinyo zakho zidinga ukukhula okufanayo kwesiko lamaseli, ungabeka amaflaski esiko ezisekelweni zensimbi ezinezindawo ezincane zokuthintana, okuvame ukungadingeki ekukhuleni kwamaseli okuvamile.
Ukunciphisa Isikhathi Sokucubungula Amaseli
Ukuze unciphise isikhathi esichithwa enkambisweni yokwelashwa kwamaseli, udinga
- ▶ Hlela zonke izinto namathuluzi adingekayo ngaphambi kokuthi uqale ukusebenza.
- ▶Sebenza ngokushesha nangokushelela, ubukeza izindlela zokuhlola kusenesikhathi ukuze imisebenzi yakho iphindaphindeke futhi izenzekele.
- ▶Nciphisa ukuthintana koketshezi nomoya ozungezile.
- ▶ Gcina izinga lokushisa lingaguquguquki elabhorethri yokukhulisa amaseli lapho usebenza khona.
Isikhathi sokuthunyelwe: Jan-03-2024