Mhedzisiro yekusiyana kwekushisa pane sero tsika
Tembiricha ndiyo yakakosha parameter mu cell tsika nekuti inokanganisa kuberekana kwemhedzisiro. Kupisa kunoshanduka pamusoro kana pasi pe37 ° C kune zvakakosha zvikuru pakukura kwesero kinetics yemasero emammalian, akafanana neaya ebhakitiriya masero. Shanduko mukutaura kwemajini uye kugadziridzwa muchimiro chemasero, kufambira mberi kwesero, kugadzikana kwemRNA kunogona kuwonekwa mumasero emammalian mushure meawa imwe pa32ºC. Pamusoro pekukanganisa zvakananga kukura kwesero, shanduko yekushisa inokanganisawo pH ye media, sezvo solubility ye CO2 inoshandura pH (pH inowedzera pakudzika kwakadzika). Maseru akakura emammalian anogona kushivirira kupisa kwakanyanya kuderera. Inogona kuchengetwa pa4 ° C kwemazuva akati wandei uye inogona kushivirira kutonhora kusvika -196 ° C (uchishandisa mamiriro akakodzera). Nekudaro, havakwanise kushivirira tembiricha iri pamusoro pe2 °C pamusoro penguva dzose kweanopfuura maawa mashoma uye vanofa nekukurumidza pa40 °C uye pamusoro. Kuti ive nechokwadi chekuberekana kwakanyanya kwemhedzisiro, kunyangwe maseru akararama, kutarisirwa kunoda kutorwa kuchengetedza tembiricha seyakagadzika sezvinobvira panguva yekuisa uye kubata kwemasero kunze kweincubator.
Zvikonzero zvekusiyana kwekushisa mukati me incubator
Iwe uchave waona kuti kana gonhi reincubator ravhurwa, tembiricha inodonha nekukurumidza kusvika pamutengo wakaiswa we37 °C. Kazhinji, tembiricha ichapora mukati memaminitsi mashoma mushure mekuvharwa kwegonhi. Muchokwadi, tsika dzakasimba dzinoda nguva yekudzoreredza kune yakatarwa tembiricha muincubator. Zvinhu zvakati wandei zvinogona kukanganisa nguva inotora kuti sero tsika idzore tembiricha mushure mekurapwa kunze kweincubator. Izvi zvinosanganisira:
- ▶hurefu hwenguva masero anga abuda muincubator
- ▶ rudzi rweflask umo maseru anokurira (geometry inokanganisa kupisa kwekushisa)
- ▶Kuwanda kwemidziyo muincubator .
- ▶Kusangana kwakananga kwemaflasks nesherufu yesimbi kunokanganisa kuchinjana kwekupisa uye nekumhanya kwekusvika kune yakanyanya tembiricha, saka zviri nani kudzivirira mirwi yeflask uye kuisa mudziyo wega wega.
- ▶zvakananga pasherufu yeincubator.
Tembiricha yekutanga yechero midziyo mitsva uye midhiya inoshandiswa inozokanganisawo nguva inotora kuti maseru ave pamamiriro awo chaiwo; iyo yakadzikira tembiricha yavo, iyo inotora nguva yakareba.
Kana zvese izvi zvinhu zvikachinja nekufamba kwenguva, ivo vanozowedzerawo musiyano pakati pekuedza. Izvo zvinodikanwa kuderedza kushanduka kwekushisa uku, kunyange kana zvisingaiti nguva dzose kudzora zvinhu zvose (kunyanya kana vanhu vakawanda vari kushandisa incubator imwechete).
Maitiro ekudzikisa kusiyanisa kwekushisa uye kuderedza tembiricha yekudzoreredza nguva
Nokupisa svikiro
Vamwe vaongorori vakajaira kudziya-pre-kudziya mabhodhoro e media mune 37 °C yekugezera yemvura kuti vauye nawo kune iyi tembiricha isati yashandiswa. Izvo zvakare zvinogoneka preheat svikiro mune incubator inongoshandiswa kune yepakati preheating uye kwete yesero tsika, uko svikiro rinogona kusvika kune yakanyanya tembiricha pasina kukanganisa sero tsika mune imwe incubator. Asi izvi, sekuziva kwedu, kazhinji haisi mari inokwanisika.
Mukati meIncubator
Vhura musuwo weincubator zvishoma sezvinobvira uye uvhare nekukurumidza. Dzivisa nzvimbo dzinotonhora, izvo zvinogadzira kusiyana kwekushisa mu incubator. Siya nzvimbo pakati pemaflask kuti mweya utenderere. Masherufu ari mukati meincubator anogona kupetwa. Izvi zvinobvumira kugovera kupisa kuri nani sezvo kuchibvumira mweya kupfuura nemakomba. Zvisinei, kuvapo kwemakomba kunogona kukonzera kusiyana kwekukura kwesero, nokuti pane kusiyana kwekushisa pakati penzvimbo ine maburi uye nzvimbo ine meta. Nezvikonzero izvi, kana zviedzo zvako zvichida kukura kwakafanana kwesero tsika, unogona kuisa mafirita etsika pazvitsigiro zvesimbi zvine madiki ekusangana, izvo zvisingawanzo kudikanwa mutsika yesero.
Kuderedza Cell Processing Nguva
Kuti uderedze nguva inopedzerwa musero kurapwa maitiro, unofanirwa kudaro
- ▶Ronga zvese zvinodiwa uye maturusi usati watanga kushanda.
- ▶Shanda nekukurumidza uye mushe, uchiongorora nzira dzekuyedza pachine nguva kuitira kuti mashandiro ako ave anodzokororwa uye otomatiki.
- ▶Cherechedza kusangana kwezvinwiwa nemhepo yakadzikama.
- ▶ Chengetedza tembiricha yenguva dzose muchitokisi tsika lab kwaunoshanda.
Nguva yekutumira: Jan-03-2024