Tasirin bambancin zafin jiki akan al'adar ƙwayoyin halitta a cikin incubator na CO2
Zafin jiki muhimmin siga ne a cikin al'adar ƙwayoyin halitta domin yana shafar sake haifar da sakamako. Canje-canjen zafin jiki sama ko ƙasa da 37°C suna da tasiri sosai akan motsin haɓakar ƙwayoyin halitta na ƙwayoyin dabbobi masu shayarwa, kamar na ƙwayoyin cuta. Canje-canje a cikin bayyanar kwayoyin halitta da gyare-gyare a cikin tsarin ƙwayoyin halitta, ci gaban zagayowar ƙwayoyin halitta, kwanciyar hankali na mRNA za a iya gano su a cikin ƙwayoyin dabbobi masu shayarwa bayan awa ɗaya a 32ºC. Baya ga shafar ci gaban ƙwayoyin halitta kai tsaye, canje-canje a cikin zafin jiki kuma yana shafar pH na kafofin watsa labarai, kamar yadda narkewar CO2 ke canza pH (pH yana ƙaruwa a ƙananan yanayin zafi). Kwayoyin dabbobi masu shayarwa da aka noma za su iya jure raguwar zafin jiki mai mahimmanci. Ana iya adana su a 4°C na tsawon kwanaki da yawa kuma suna iya jure daskarewa zuwa -196 °C (ta amfani da yanayi masu dacewa). Duk da haka, ba za su iya jure yanayin zafi sama da 2 °C sama da na al'ada ba na tsawon awanni fiye da 'yan awanni kuma za su mutu da sauri a 40 °C da sama. Don tabbatar da mafi girman sake haifar da sakamako, koda kuwa ƙwayoyin sun tsira, ana buƙatar kulawa don kiyaye zafin jiki gwargwadon iko yayin shiryawa da sarrafa ƙwayoyin da ke wajen incubator.
Babban dalilai na canjin zafin jiki a cikin incubator
Za ka lura cewa idan aka buɗe ƙofar incubator, zafin jiki yana raguwa da sauri zuwa ƙimar da aka saita na 37 °C. Gabaɗaya, zafin jiki zai dawo cikin 'yan mintuna kaɗan bayan an rufe ƙofar. A zahiri, al'adu masu tsauri suna buƙatar lokaci don murmurewa zuwa zafin da aka saita a cikin incubator. Abubuwa da yawa na iya shafar lokacin da al'adar tantanin halitta ke ɗauka don sake samun zafin jiki bayan an yi magani a wajen incubator. Waɗannan abubuwan sun haɗa da:
- ▶ Tsawon lokacin da ƙwayoyin suka fita daga cikin incubator
- ▶ nau'in kwalbar da ake girma ƙwayoyin halitta a ciki (geometry yana shafar canja wurin zafi)
- ▶Yawan kwantena a cikin incubator.
- ▶ Shafar kwalbar kai tsaye da shiryayyen ƙarfe yana shafar musayar zafi da saurin isa ga mafi kyawun zafin jiki, don haka ya fi kyau a guji tarin kwalbar a sanya kowace kwalba
- ▶ kai tsaye a kan shiryayyen incubator.
Zafin farko na kowace sabuwar kwantena da kayan da aka yi amfani da su zai kuma shafi lokacin da ƙwayoyin za su kasance a cikin yanayin da ya fi dacewa; ƙarancin zafinsu, haka nan yake ɗaukar lokaci mai tsawo.
Idan duk waɗannan abubuwan suka canza akan lokaci, za su kuma ƙara yawan bambancin da ke tsakanin gwaje-gwaje. Ya zama dole a rage waɗannan canjin yanayin zafi, koda kuwa ba koyaushe zai yiwu a sarrafa komai ba (musamman idan mutane da yawa suna amfani da irin wannanInjin samar da iskar CO2).
Yadda ake rage bambancin zafin jiki da kuma rage lokacin dawo da zafin jiki
Ta hanyar dumama matsakaiciyar
Wasu masu bincike sun saba da ɗumamar kwalaben watsawa gaba ɗaya a cikin ruwan wanka mai zafin digiri 37 na Celsius don kawo su zuwa wannan zafin kafin amfani. Haka kuma yana yiwuwa a dumama matsakaiciyar a cikin incubator wanda ake amfani da shi kawai don dumama matsakaici ba don al'adar ƙwayoyin halitta ba, inda matsakaiciyar za ta iya isa ga mafi kyawun zafin jiki ba tare da dagula al'adun ƙwayoyin halitta a cikin wani incubator ba. Amma wannan, gwargwadon yadda muka sani, yawanci ba kuɗi ne mai araha ba.
A Cikin Incubator
Buɗe ƙofar incubator gwargwadon iyawa sannan a rufe ta da sauri. A guji wuraren sanyi, waɗanda ke haifar da bambance-bambancen zafin jiki a cikin incubator. A bar sarari tsakanin kwalaben don barin iska ta zagaya. Ana iya huda shelf ɗin da ke cikin incubator. Wannan yana ba da damar rarraba zafi mafi kyau saboda yana ba da damar iska ta ratsa ramukan. Duk da haka, kasancewar ramuka na iya haifar da bambance-bambance a cikin girman ƙwayoyin halitta, saboda akwai bambancin zafin jiki tsakanin yankin da ke da ramuka da yankin da ke da meta. Saboda waɗannan dalilai, idan gwaje-gwajenku suna buƙatar girma iri ɗaya na al'adar ƙwayoyin halitta, za ku iya sanya kwalaben al'ada a kan tallafin ƙarfe tare da ƙananan saman hulɗa, waɗanda yawanci ba lallai ba ne a cikin al'adar ƙwayoyin halitta ta yau da kullun.
Rage Lokacin Sarrafa Tantanin Halitta
Don rage lokacin da ake kashewa a cikin tsarin maganin ƙwayoyin cuta, kuna buƙatar
- ▶ Shirya dukkan kayan aiki da kayan aiki da ake buƙata kafin fara aiki.
- ▶ Yi aiki da sauri da sauƙi, duba hanyoyin gwaji a gaba domin ayyukanka su zama masu maimaitawa da kuma atomatik.
- ▶ Rage hulɗar ruwa da iskar da ke kewaye.
- ▶ Kiyaye yanayin zafin jiki a dakin gwaje-gwajen tantance ƙwayoyin halitta inda kike aiki.
Lokacin Saƙo: Janairu-03-2024