Tasirin bambancin zafin jiki akan al'adar tantanin halitta
Zazzabi shine muhimmin ma'auni a al'adun tantanin halitta saboda yana shafar sake haifar da sakamako. Canjin yanayin zafi sama ko ƙasa da 37 ° C yana da matukar tasiri akan haɓakar haɓakar tantanin halitta na sel masu shayarwa, kama da na ƙwayoyin cuta. Canje-canje a cikin maganganun kwayoyin halitta da gyare-gyare a cikin tsarin salula, ci gaban sake zagayowar tantanin halitta, ana iya gano kwanciyar hankali na mRNA a cikin sel masu shayarwa bayan sa'a daya a 32ºC. Bugu da ƙari, kai tsaye ya shafi ci gaban cell, canje-canje a cikin zafin jiki kuma yana shafar pH na kafofin watsa labaru, kamar yadda solubility na CO2 ya canza pH (pH yana ƙaruwa a ƙananan yanayin zafi). Kwayoyin dabbobi masu shayarwa na iya jurewa gagarumin raguwar zafin jiki. Ana iya adana su a 4 ° C na kwanaki da yawa kuma suna iya jure wa daskarewa zuwa -196 ° C (ta amfani da yanayin da suka dace). Duk da haka, ba za su iya jure wa yanayin zafi sama da 2 ° C sama da al'ada ba fiye da ƴan sa'o'i kuma za su mutu da sauri a 40 ° C da sama. Don tabbatar da iyakar sake haifuwa na sakamako, ko da sel sun tsira, ana buƙatar kulawa don kula da zafin jiki gwargwadon yuwuwar lokacin shiryawa da sarrafa sel a wajen incubator.
Dalilan bambancin zafin jiki a cikin incubator
Za ku lura cewa lokacin da aka buɗe ƙofar incubator, zafin jiki yana raguwa da sauri zuwa ƙimar da aka saita na 37 ° C. Gabaɗaya, zafin jiki zai dawo cikin 'yan mintuna kaɗan bayan an rufe ƙofar. A haƙiƙa, al'adu na tsaye suna buƙatar lokaci don murmurewa zuwa yanayin da aka saita a cikin incubator. Abubuwa da yawa na iya shafar lokacin da al'adar tantanin halitta ke ɗauka don dawo da zafin jiki bayan jiyya a wajen incubator. Waɗannan abubuwan sun haɗa da:
- ▶ tsawon lokacin da sel suka fita daga cikin incubator
- ▶ nau'in flask ɗin da ake girma a cikin sel (geometry yana rinjayar yanayin zafi)
- ▶Yawan kwantena a cikin incubator .
- ▶ Kai tsaye hulɗar flasks tare da shiryayye na karfe yana rinjayar musayar zafi da saurin isa ga mafi kyawun zafin jiki, don haka yana da kyau a guje wa tarin flasks da sanya kowane jirgin ruwa.
- ▶ kai tsaye a kan shiryayye na incubator.
Zazzabi na farko na kowane sabon kwantena da kafofin watsa labaru da aka yi amfani da su kuma zai shafi lokacin da ake ɗauka don sel su kasance a mafi kyawun yanayin su; rage yawan zafin jiki, tsawon lokacin yana ɗauka.
Idan duk waɗannan abubuwan sun canza akan lokaci, za su kuma ƙara haɓaka tsakanin gwaje-gwaje. Wajibi ne a rage girman waɗannan sauye-sauyen zafin jiki, koda kuwa ba koyaushe yana yiwuwa a sarrafa komai ba (musamman idan mutane da yawa suna amfani da incubator iri ɗaya).
Yadda ake rage bambance-bambancen zafin jiki da rage lokacin dawo da zafin jiki
Ta hanyar preheating matsakaici
Wasu masu binciken sun saba da yin dumama dukkan kwalabe na kafofin watsa labarai a cikin ruwan wanka na 37 ° C don kawo su zuwa wannan zafin jiki kafin amfani. Hakanan yana yiwuwa a yi zafi mai matsakaici a cikin incubator wanda ake amfani da shi kawai don matsakaicin zafin jiki ba don al'adun tantanin halitta ba, inda matsakaicin zai iya kaiwa yanayin zafi mai kyau ba tare da damun al'adun tantanin halitta a cikin wani incubator ba. Amma wannan, kamar yadda muka sani, yawanci ba tsada ba ne.
Ciki da Incubator
Bude kofar incubator kadan kadan mai yiwuwa kuma rufe shi da sauri. Ka guji wuraren sanyi, waɗanda ke haifar da bambance-bambancen zafin jiki a cikin incubator. Bar sarari tsakanin faifan don ba da damar iska ta zagaya. Za a iya rarrabu da shelves a cikin incubator. Wannan yana ba da damar rarraba zafi mafi kyau yayin da yake ba da damar iska ta ratsa cikin ramuka. Duk da haka, kasancewar ramuka na iya haifar da bambance-bambance a cikin ci gaban sel, saboda akwai bambancin zafin jiki tsakanin yankin da ramuka da yanki tare da meta. Don waɗannan dalilai, idan gwaje-gwajen ku na buƙatar haɓakar haɓakar al'adun tantanin halitta sosai, zaku iya sanya flasks na al'ada akan goyan bayan ƙarfe tare da ƙananan filaye masu alaƙa, waɗanda yawanci ba lallai bane a al'adun tantanin halitta na yau da kullun.
Rage Lokacin Gudanar da Tantanin halitta
Don rage lokacin da ake kashewa a cikin tsarin jiyya ta salula, kuna buƙatar
- ▶ Shirya duk kayan aikin da ake buƙata kafin fara aiki.
- ▶ Yi aiki cikin sauri da sauƙi, yin bitar hanyoyin gwaji a gaba don ayyukanku su zama masu maimaitawa da sarrafa kansu.
- ▶ Rage hulɗar ruwa tare da iskar yanayi.
- ▶Kiyaye yawan zafin jiki a dakin gwaje-gwajen al'adun tantanin halitta inda kuke aiki.
Lokacin aikawa: Janairu-03-2024