Ingaruka zo guhindagurika k'ubushyuhe ku muco w'akagari
Ubushyuhe nibintu byingenzi mumico y'akagari kuko bigira ingaruka kubyara ibisubizo. Imihindagurikire yubushyuhe hejuru cyangwa munsi ya 37 ° C igira ingaruka zikomeye kumikurire yimikorere ya selile yinyamabere, zisa na selile. Impinduka mu mvugo ya gene no guhindura imiterere ya selile, iterambere ryingirabuzimafatizo, mRNA ihagaze neza irashobora kugaragara mungirangingo zinyamabere nyuma yisaha imwe kuri 32ºC. Usibye kugira ingaruka zitaziguye ku mikurire ya selile, impinduka zubushyuhe nazo zigira ingaruka kuri pH yibitangazamakuru, kuko gukomera kwa CO2 bihindura pH (pH yiyongera kubushyuhe buke). Ingirabuzimafatizo z’inyamabere zifite umuco zirashobora kwihanganira ubushyuhe bugabanuka. Birashobora kubikwa kuri 4 ° C muminsi myinshi kandi birashobora kwihanganira ubukonje kugeza kuri -196 ° C (ukoresheje ibihe bikwiye). Ariko, ntibashobora kwihanganira ubushyuhe buri hejuru ya 2 ° C hejuru yubusanzwe mumasaha arenze amasaha make kandi bazapfa vuba kuri 40 ° C no hejuru. Kugirango hamenyekane umusaruro mwinshi wibisubizo, nubwo selile zibaho, hagomba kwitonderwa kugirango ubushyuhe bugumane burigihe bushoboka mugihe cyo gukora no gufata ingirabuzimafatizo hanze ya incubator.
Impamvu zubushyuhe butandukanye imbere muri incubator
Uzaba wabonye ko iyo umuryango wa incubator ufunguye, ubushyuhe bugabanuka vuba kugeza ku giciro cyagenwe cya 37 ° C. Muri rusange, ubushyuhe buzasubirana muminota mike nyuma yumuryango ufunze. Mubyukuri, imico ihamye ikenera igihe cyo gukira ubushyuhe bwashyizweho muri incubator. Impamvu zitari nke zirashobora kugira ingaruka kumwanya bifata kugirango umuco w'akagari ugarure ubushyuhe nyuma yo kuvurwa hanze ya incubator.Ibintu birimo ：
- Length uburebure bwigihe selile zasohotse muri incubator
- Ubwoko bwa flask aho ingirabuzimafatizo zikurira (geometrie igira ingaruka kubushyuhe)
- ▶ Umubare wibikoresho muri incubator.
- Guhuza neza na flask hamwe nicyuma cyicyuma bigira ingaruka kumyuka yubushyuhe n'umuvuduko wo kugera ku bushyuhe bwiza, nibyiza rero kwirinda ibirindiro bya flash hanyuma ugashyira buri cyombo
- ▶ mu buryo butaziguye ku gipangu cya incubator.
Ubushyuhe bwambere bwibintu byose bishya nibitangazamakuru byakoreshejwe nabyo bizagira ingaruka kumwanya bifata kugirango selile zibe nziza; munsi ubushyuhe bwabo, burigihe bifata.
Niba ibyo bintu byose bihindutse mugihe, bizongera kandi itandukaniro riri hagati yubushakashatsi. Birakenewe kugabanya ihindagurika ryubushyuhe, nubwo bidashoboka buri gihe kugenzura ibintu byose (cyane cyane niba abantu benshi bakoresha incubator imwe).
Nigute wagabanya ubushyuhe butandukanye no kugabanya igihe cyo kugarura ubushyuhe
Mugushushanya uburyo
Abashakashatsi bamwe bamenyereye mbere yo gushyushya amacupa yose yibitangazamakuru mu bwogero bw’amazi 37 ° C kugirango babizane kuri ubu bushyuhe mbere yo kubikoresha. Birashoboka kandi gushyushya uburyo muri incubator ikoreshwa gusa mubushuhe buciriritse kandi ntabwo ari umuco w'akagari, aho uburyo bushobora kugera ku bushyuhe bwiza butabangamiye imico y'utugari muyindi incubator. Ariko ibi, nkuko tubizi, mubisanzwe ntabwo ari ikiguzi gihenze.
Imbere muri Incubator
Fungura umuryango wa incubator bike bishoboka kandi ufunge vuba. Irinde ibibanza bikonje, bitera itandukaniro ryubushyuhe muri incubator. Siga umwanya hagati ya flasike kugirango umwuka uzenguruke. Isahani imbere muri incubator irashobora gutoborwa. Ibi bituma habaho gukwirakwiza ubushyuhe bwiza kuko butuma umwuka unyura mu mwobo. Ariko, kuba hari ibyobo bishobora kuganisha ku itandukaniro ryimikurire ya selile, kuko hariho itandukaniro ryubushyuhe hagati yakarere hamwe nu mwobo hamwe na meta. Kubera izo mpamvu, niba ubushakashatsi bwawe busaba gukura cyane kumico yutugari, urashobora gushyira flasque yumuco kumurongo wibyuma hamwe nuduce duto two guhuza, ubusanzwe ntibikenewe mumico isanzwe.
Kugabanya Igihe cyo Gutunganya Akagari
Kugabanya igihe umara mugikorwa cyo kuvura selile, ugomba
- Tegura ibikoresho byose bikenewe mbere yuko utangira gukora.
- ▶ Kora vuba kandi neza, usubiremo uburyo bwo kugerageza hakiri kare kugirango ibikorwa byawe bisubiremo kandi byikora.
- Kugabanya guhura kwamazi hamwe numwuka w ibidukikije.
- Komeza ubushyuhe burigihe muri laboratoire yumuco aho ukorera.
Igihe cyo kohereza: Mutarama-03-2024