Yuav tsum ntxiv ntau npaum li cas rau hauv lub raj mis hauv lub tshuab co?
RADOBIO sterile cell culture Erlenmeyer flasks yog tsim tshwj xeeb rau kev cog cov hlwb ncua suav nrog HEK 293 thiab CHO hlwb hauv ib qholub tshuab ua kom coCov fwj no muaj lub hauv qab tiaj tus thiab lub hau uas muaj qhov cua, ua rau lawv haum rau kev txhim kho cov txheej txheem me me, kev nthuav dav, thiab kev cog qoob loo ntau theem. Lub hau uas muaj qhov cua sib xyaw ua ke nrog 0.2 μm hydrophobic membrane, uas ua rau muaj kev sib pauv roj zoo thaum thaiv cov kab mob me me thiab kev nkag mus rau hauv dej, ua kom ntseeg tau tias cov xwm txheej zoo tshaj plaws ntawm kev loj hlob ntawm cov hlwb.
Cov Lus Qhia Tseem Ceeb thiab Cov Lus Qhia Siv
- 1. Kev Txhim Kho Qhov Ntim Ua Haujlwm
- •Qhov ntim nruab nrab yuav tsum tau tswj kom nyob ntawm 20–30% ntawm tag nrho lub peev xwm ntawm lub raj mis kom ntseeg tau tias muaj kev xa oxygen txaus thiab txo qhov kev ntxhov siab ntawm kev txiav.
- • Cov cim qhia meej ntawm lub raj mis ua rau ntsuas qhov ntim tau meej .
- •Qhov ntim nruab nrab yuav tsum tau tswj kom nyob ntawm 20–30% ntawm tag nrho lub peev xwm ntawm lub raj mis kom ntseeg tau tias muaj kev xa oxygen txaus thiab txo qhov kev ntxhov siab ntawm kev txiav.
- 2. Cov Kev Ntsuas Kev Co
- •Pom zoolub tshuab ua kom ...Kev co ceev: 75–120 RPM (kho tau raws li kev xav tau ntawm kev sim)
- •Rau cov fwj loj dua (≥3L), txo qhov ceev mus rau 90 RPM kom tiv thaiv cov kua ntau dhau thiab ua npuas dej.
- •Pom zoolub tshuab ua kom ...Kev co ceev: 75–120 RPM (kho tau raws li kev xav tau ntawm kev sim)
Cov Qauv Pom Zoo rau CHO & HEK 293 Cells
| Qhov Ntim ntawm Lub Raj Mis | Ua Haujlwm Ntim | Kev co ceev |
|---|---|---|
| 125 ml | 25 mL | 120 RPM |
| 250 ml | 60 ml | 120 RPM |
| 500 ml | 150 ml | 120 RPM |
| 1000 ml | 350 ml | 120 RPM |
| 1500 ml | 400 ml | 120 RPM |
| 2000 ml | 400 ml | 120 RPM |
| 3000 ml | 800 ml | 90 RPM |
| 5000 ml | 2500 ml | 90 RPM |
Lus Cim: Cov ntaub ntawv siv rau CHO Stable, CHO Transient, thiab HEK 293 Transient cell lines
Cov Ntaub Ntawv Ntxiv Txog Kev Siv Tshuab
- 1. Khoom siv thiab kev tsis muaj menyuam
- • Cov Khoom Siv PETG / PC:PETG muaj kev pom tseeb thiab kev cuam tshuam tsis kam; PC muab kev ruaj khov thermal zoo dua (tiv taus autoclaving ntawm 121 ° C)
-
- •Kev Ua Kom Tsis Muaj Kab Mob: Gamma-irradiated, ntim ib leeg, tau ntawv pov thawj DNase/RNase-dawb, endotoxin-dawb, thiab ua raws li USP Class VI tus qauv
- • Cov Khoom Siv PETG / PC:PETG muaj kev pom tseeb thiab kev cuam tshuam tsis kam; PC muab kev ruaj khov thermal zoo dua (tiv taus autoclaving ntawm 121 ° C)
- 2.Qhov Zoo ntawm Kev Tsim Qauv
- •Lub hau Ergonomic: Pab txhawb kev tuav pov hwm thiab kev sib raug zoo nrog cov pipettes / cell scrapers
- •Cov Qauv Baffled Muaj: Txhim kho kev sib xyaw ua kom zoo rau cov kab lis kev cai siab los ntawm kev nce kev hloov pauv oxygen (kLa)
- •Lub hau Ergonomic: Pab txhawb kev tuav pov hwm thiab kev sib raug zoo nrog cov pipettes / cell scrapers
- 3. Cov Lus Cim Tseem Ceeb Txog Kev Siv
- •Tsis txhob ntim ntau dhau: Qhov ntim ntau dua 30% yuav txo qhov kev sib pauv roj, ua rau hypoxia hauv cov kab lis kev cai aerobic
- •Saib xyuas pH/Oxygen: Tswj pH ntawm 7.0–7.4 (CHO hlwb) thiab cov pa oxygen yaj (DO) >40% rau kev ua kom lub cev zom zaub mov zoo tshaj plaws
- •Kev Ruaj Ntseg: Rau cov hlwb uas rhiab heev rau kev raug mob (piv txwv li, cov hlwb T thawj zaug), siv cov kev ceev qis dua (80–100 RPM) lossis cov tshuaj tiv thaiv ua npuas ncauj
- •Tsis txhob ntim ntau dhau: Qhov ntim ntau dua 30% yuav txo qhov kev sib pauv roj, ua rau hypoxia hauv cov kab lis kev cai aerobic
Cov Ntawv Thov Tshaj Lij Kev Cog Qoob Loo
- •Cov Txheej Txheem Perfusion: Sib koom ua ke nrog cov kab pub rau kev muab cov as-ham tas mus li, ua tiav cov cell density >20 × 10⁶ cells/mL
- •Kev Kab Mob Me Me: Haum rau kev ua kom cov kab mob/yeast fermentation nrog cov qauv tsim baffled rau kev ua kom zoo dua aeration
- •Cov Txheej Txheem Nce Qib: Thaum hloov ntawm cov fwj mus rau bioreactors, tswj kom muaj qhov sib xws ntawm volumetric oxygen transfer rate (kLa) los ntawm kev kho qhov kev ntxhov siab thiab aeration.
Lub sijhawm tshaj tawm: Lub Yim Hli-17-2025