E hia te nui o te wai hei tāpiri atu ki te pounamu i roto i te pūwero rūrū?
He mea hanga motuhake ngā ipu Erlenmeyer ahurea pūtau parakore a RADOBIO mō te whakatipu i ngā pūtau whakatārewatanga tae atu ki ngā pūtau HEK 293 me CHO i roto i tete whakapūkara wiriHe papatahi te raro o ēnei ipu me te taupoki hau, e pai ai mō te whakawhanaketanga tukanga iti, te whakanui ake, me te whakatipu maha-wāhanga. Kei roto i te taupoki hau he kiriuhi wai-whakaaro 0.2 μm, e āhei ai te whakawhiti hau pai me te aukati i te poke o ngā moroiti me te urunga wai, e whakarite ana i ngā āhuatanga tipu pūtau tino pai.
Ngā Whakatakotoranga Matua me ngā Aratohu Whakamahinga
- 1.Te Arotau i te Rōrahi Mahi'
- •Me pupuri te rōrahi waenga ki te 20–30% o te katoa o te kaha o te pounamu kia pai ai te whakawhiti hāora me te whakaiti i te ahotea kutikuti.
- •Mā ngā tohu mārama i runga i te pounamu ka taea te ine tika i te rōrahi .
- •Me pupuri te rōrahi waenga ki te 20–30% o te katoa o te kaha o te pounamu kia pai ai te whakawhiti hāora me te whakaiti i te ahotea kutikuti.
- 2. Ngā Tawhā Rūrū'
- •I tūtohutiarūrū whakapūkaratere wiri: 75–120 RPM (ka taea te whakarerekē i runga i ngā hiahia whakamātautau)
- •Mō ngā ipu nui ake (≥3L), whakaitihia te tere ki te 90 RPM hei ārai i te kutikuti wai nui me te huka.
- •I tūtohutiarūrū whakapūkaratere wiri: 75–120 RPM (ka taea te whakarerekē i runga i ngā hiahia whakamātautau)
Ngā Tawhā e Manakohia ana mō ngā Pūtau CHO me HEK 293
| Rōrahi o te Ipu | Rōrahi Mahi | Tere Rūrū |
|---|---|---|
| 125 mL | 25 mL | 120 RPM |
| 250 mL | 60 mL | 120 RPM |
| 500 mL | 150 mL | 120 RPM |
| 1000 mL | 350 mL | 120 RPM |
| 1500 mL | 400 mL | 120 RPM |
| 2000 mL | 400 mL | 120 RPM |
| 3000 mL | 800 mL | 90 RPM |
| 5000 mL | 2500 mL | 90 RPM |
Kia mōhio: E pā ana ngā raraunga ki ngā raina pūtau CHO Stable, CHO Transient, me HEK 293 Transient
Ngā Taipitopito Hangarau Tāpiri
- 1. Rauemi me te Whai Huakore'
- •Ngā Kōwhiringa Rauemi PETG/PC:He tino mārama te PETG me te ātete ki te pānga; he tino pai te pumau o te PC (ka ātete ki te whakamahana i te 121°C)
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- •Te Whakahoromata: Kua whakamāramahia ki te gamma, kua takai takitahi, kua whaimanahia he kore DNase/RNase, he kore endotoxin, ā, e tutuki ana i ngā paerewa USP Akomanga VI.
- •Ngā Kōwhiringa Rauemi PETG/PC:He tino mārama te PETG me te ātete ki te pānga; he tino pai te pumau o te PC (ka ātete ki te whakamahana i te 121°C)
- 2. Ngā Painga Hoahoa'
- •Taopenga Ergonomic: Mā te whakamahi haumaru me te hototahi ki ngā pipettes/ngā waru pūtau
- •Ngā Tauira Pōhēhē e Wātea ana: Whakarei ake i te pai o te whakaranu mō ngā ahurea kiato-teitei mā te whakanui ake i te whakawhiti hāora (kLa)
- •Taopenga Ergonomic: Mā te whakamahi haumaru me te hototahi ki ngā pipettes/ngā waru pūtau
- 3.Ngā Tuhipoka Whakamahinga Hira'
- •Kaua e Whakakī Rawa: Ko ngā rahinga neke atu i te 30% ka whakaiti i te whakawhiti hau, ka arahi ki te hypoxia i roto i ngā ahurea aerobic.
- •Aroturuki i te pH/Hāora: Kia mau tonu te pH ki te 7.0–7.4 (ngā pūtau CHO) me te hāora rewa (DO) >40% mō te pākia pai o te tinana
- •Te Tairongo Kutikuti: Mō ngā pūtau tairongo kutikuti (hei tauira, ngā pūtau T tuatahi), whakamahia ngā tere iti iho (80–100 RPM) me ngā kaihoko ārai-huka rānei.
- •Kaua e Whakakī Rawa: Ko ngā rahinga neke atu i te 30% ka whakaiti i te whakawhiti hau, ka arahi ki te hypoxia i roto i ngā ahurea aerobic.
Ngā Taupānga i tua atu i te Whakatipu Paerewa
- •Ngā Pūnaha Whakarewa: Whakauruhia ki ngā raina whāngai mō te tuku matūkai tonu, kia eke ki te kiato pūtau >20 × 10⁶ pūtau/mL
- •Ngā Ahurea moroiti: He pai mō te whakapūtanga huakita/rewena me ngā hoahoa pōhēhē hei whakapai ake i te hau.
- •Ngā Tikanga Whakanui Ake: I te whakawhiti mai i ngā pounamu ki ngā matū koiora, kia mau tonu te tere whakawhiti hāora (kLa) mā te whakarerekē i te whakaoho me te whakapūngao hau.
Wā tuku: 17 o Ākuhata-2025